##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
AspI BsaBI BseAI BsiWI BsmBI
BspDI BspEI BspHI BsrBI Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
Eco47III EcoO65I EcoRV EcoT22I FseI
HincII HindII HpaI KpnI KspI
MamI MluI MroI NaeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SfiI SgrAI
SmaI SnaBI SspI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflII 788 CTTAAG C^TTAAG
AhdI 4959 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 343 GTGCAC G^TGCAC
ApaLI 343 GTGCAC G^TGCAC
Asp700I 1699 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4959 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2614 TGCGCA TGC^GCA
BamHI 727 GGATCC G^GATCC
BbeI 740 GGCGCC GGCGC^C
BbrPI 2979 CACGTG CAC^GTG
BbvCI 5047 GCTGAGG CCTCAGC(-5/-2)
BcgI 4509 GCAGCTCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2786 GGATAC GTATCC(6/5)
BfrI 788 CTTAAG C^TTAAG
BglII 5280 AGATCT A^GATCT
BsaAI 2979 CACGTG [CT]AC^GT[GA]
BsaWI 362 ACCGGA [AT]^CCGG[AT]
BsiEI 600 CGGCCG CG[GA][CT]^CG
BssHII 5603 GCGCGC G^CGCGC
BstBI 104 TTCGAA TT^CGAA
DrdI 1034 GACTATATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 600 CGGCCG C^GGCCG
Eam1105I 4959 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 600 CGGCCG C^GGCCG
Eco52I 600 CGGCCG C^GGCCG
FauI 167 CCCGC CCCGC(4/6)
FspI 2614 TGCGCA TGC^GCA
KasI 740 GGCGCC G^GCGCC
NarI 740 GGCGCC GG^CGCC
NdeI 3368 CATATG CA^TATG
NspV 104 TTCGAA TT^CGAA
PmaCI 2979 CACGTG CAC^GTG
PmlI 2979 CACGTG CAC^GTG
SexAI 1274 ACCTGGT A^CC[AT]GGT
SfoI 740 GGCGCC GGC^GCC
SfuI 104 TTCGAA TT^CGAA
SpeI 1985 ACTAGT A^CTAGT
StuI 4154 AGGCCT AGG^CCT
XmnI 1699 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 186 GTCTAC GT^[AC][GT]AC
1738 GTAGAC
AccII 5604 CGCG CG^CG
5606 CGCG
AcyI 740 GGCGCC G[GA]^CG[CT]C
2712 GGCGTC
BclI 1024 TGATCA T^GATCA
4276 TGATCA
BlpI 3392 GCTCAGC GC^T[ACGT]AGC
3735 GCTCAGC
Bpu1102I 3392 GCTCAGC GC^T[ACGT]AGC
3735 GCTCAGC
BsaHI 740 GGCGCC G[GA]^CG[CT]C
2712 GGCGTC
BspLU11I 514 ACATGT A^CATGT
3008 ACATGT
BssSI 780 CTCGTG CACGAG(-5/-1)
2825 CTCGTG
BstAPI 534 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1253 GCATCCATTGC
BstUI 5604 CGCG CG^CG
5606 CGCG
CelII 3392 GCTCAGC GC^T[ACGT]AGC
3735 GCTCAGC
EcoRI 2931 GAATTC G^AATTC
5376 GAATTC
FbaI 1024 TGATCA T^GATCA
4276 TGATCA
HaeII 740 GGCGCC [GA]GCGC^[CT]
3538 GGCGCT
Hin1I 740 GGCGCC G[GA]^CG[CT]C
2712 GGCGTC
HindIII 100 AAGCTT A^AGCTT
4103 AAGCTT
MfeI 2917 CAATTG C^AATTG
5580 CAATTG
MunI 2917 CAATTG C^AATTG
5580 CAATTG
MvnI 5604 CGCG CG^CG
5606 CGCG
SapI 3764 GCTCTTC GCTCTTC(1/4)
3984 GCTCTTC
SbfI 573 CCTGCAGG CCTGCA^GG
2432 CCTGCAGG
Sse8387I 573 CCTGCAGG CCTGCA^GG
2432 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BlnI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII