##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccIII AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbrPI BcgI BclI
BglI BlnI BseAI BsiEI BsiWI
BspEI BsrBI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FbaI FseI FspI
KspI MfeI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflMI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I Van91I XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 1454 GGTACC G^GTACC
AclI 3595 AACGTT AA^CGTT
AflII 14 CTTAAG C^TTAAG
AgeI 1431 ACCGGT A^CCGGT
ApaI 4051 GGGCCC GGGCC^C
Asp718I 1454 GGTACC G^GTACC
BbeI 1918 GGCGCC GGCGC^C
BciVI 3675 GGATAC GTATCC(6/5)
BfrI 14 CTTAAG C^TTAAG
BlpI 2137 GCTGAGC GC^T[ACGT]AGC
BpmI 2288 CTGGAG CTGGAG(16/14)
Bpu1102I 2137 GCTGAGC GC^T[ACGT]AGC
BsaAI 4411 CACGTA [CT]AC^GT[GA]
BsaBI 2784 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2429 GAGACC GGTCTC(1/5)
BspDI 875 ATCGAT AT^CGAT
BsrFI 1431 ACCGGT [GA]^CCGG[CT]
CelII 2137 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1431 ACCGGT [GA]^CCGG[CT]
ClaI 875 ATCGAT AT^CGAT
DraIII 2891 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2453 CCTACACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 5053 GATATC GAT^ATC
EcoT22I 3527 ATGCAT ATGCA^T
HaeII 1918 GGCGCC [GA]GCGC^[CT]
HpaI 4583 GTTAAC GTT^AAC
KasI 1918 GGCGCC G^GCGCC
KpnI 1454 GGTACC GGTAC^C
MamI 2784 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1770 ACGCGT A^CGCGT
NarI 1918 GGCGCC GG^CGCC
NcoI 1028 CCATGG C^CATGG
NsiI 3527 ATGCAT ATGCA^T
PinAI 1431 ACCGGT A^CCGGT
Psp1406I 3595 AACGTT AA^CGTT
PvuII 380 CAGCTG CAG^CTG
SfoI 1918 GGCGCC GGC^GCC
SwaI 4217 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 535 GTCTAC GT^[AC][GT]AC
2993 GTAGAC
AccII 1024 CGCG CG^CG
1771 CGCG
AocI 1985 CCTCAGG CC^T[ACGT]AGG
3445 CCTCAGG
AspI 1226 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2467 GACTGTGTC
BalI 3042 TGGCCA TGG^CCA
3346 TGGCCA
BglII 3717 AGATCT A^GATCT
4210 AGATCT
BsmBI 100 CGTCTC CGTCTC(1/5)
722 GAGACG
BsmI 4813 GAATGC GAATGC(1/-1)
4065 GCATTC
Bsp1407I 3915 TGTACA T^GTACA
4454 TGTACA
BsrGI 3915 TGTACA T^GTACA
4454 TGTACA
BstUI 1024 CGCG CG^CG
1771 CGCG
BstXI 52 CCACAGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3867 CCACTGACCTGG
Bsu36I 1985 CCTCAGG CC^T[ACGT]AGG
3445 CCTCAGG
CfoI 1025 GCGC GCG^C
1919 GCGC
DsaI 1028 CCATGG C^C[GA][CT]GG
4962 CCACGG
Eco81I 1985 CCTCAGG CC^T[ACGT]AGG
3445 CCTCAGG
HhaI 1025 GCGC GCG^C
1919 GCGC
HinP1I 1025 GCGC G^CGC
1919 GCGC
MluNI 3042 TGGCCA TGG^CCA
3346 TGGCCA
MscI 3042 TGGCCA TGG^CCA
3346 TGGCCA
MvnI 1024 CGCG CG^CG
1771 CGCG
PaeR7I 1095 CTCGAG C^TCGAG
4315 CTCGAG
PflFI 1226 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2467 GACTGTGTC
SacI 3533 GAGCTC GAGCT^C
4312 GAGCTC
SspBI 3915 TGTACA T^GTACA
4454 TGTACA
Tth111I 1226 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2467 GACTGTGTC
XhoI 1095 CTCGAG C^TCGAG
4315 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AatII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpuAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspLU11I BspMI BsrDI BsrI
BssKI BssSI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI Hin1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RcaI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI XmnI