##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AclI AcyI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI BbeI BbrPI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FseI
Hin1I KasI KspI MamI MluI
MroI NaeI NarI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SspI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 5419 GTCGAC GT^[AC][GT]AC
AflII 4621 CTTAAG C^TTAAG
AgeI 2645 ACCGGT A^CCGGT
Alw44I 5273 GTGCAC G^TGCAC
ApaLI 5273 GTGCAC G^TGCAC
AvaI 1038 CCCGAG C^[CT]CG[GA]G
AviII 4830 TGCGCA TGC^GCA
BamHI 1022 GGATCC G^GATCC
BcgI 1811 GCATTGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4621 CTTAAG C^TTAAG
BglI 1860 GCCTCTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3980 AGATCT A^GATCT
BsaWI 2645 ACCGGT [AT]^CCGG[AT]
BsmBI 260 CGTCTC CGTCTC(1/5)
BsoBI 1038 CCCGAG C^[CT]CG[GA]G
BspLU11I 4317 ACATGT A^CATGT
BsrBI 645 GAGCGG CCGCTC(-3/-3)
BssSI 239 CACGAG CACGAG(-5/-1)
BstEII 1323 GGTGACC G^GT[ACGT]ACC
BstPI 1323 GGTGACC G^GT[ACGT]ACC
DraI 5467 TTTAAA TTT^AAA
DraIII 4395 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1323 GGTGACC G^GT[ACGT]ACC
EcoT22I 5460 ATGCAT ATGCA^T
FspI 4830 TGCGCA TGC^GCA
HgaI 2154 GACGC GACGC(5/10)
HindIII 1093 AAGCTT A^AGCTT
HpaI 4813 GTTAAC GTT^AAC
MfeI 1920 CAATTG C^AATTG
MunI 1920 CAATTG C^AATTG
NsiI 5460 ATGCAT ATGCA^T
PinAI 2645 ACCGGT A^CCGGT
SalI 5419 GTCGAC G^TCGAC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2382 GGTACC G^GTACC
4042 GGTACC
AccII 2224 CGCG CG^CG
3828 CGCG
ApaI 1881 GGGCCC GGGCC^C
3402 GGGCCC
Asp700I 141 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2811 GAAATTGTTC
Asp718I 2382 GGTACC G^GTACC
4042 GGTACC
AvrII 4005 CCTAGG C^CTAGG
4966 CCTAGG
BalI 1839 TGGCCA TGG^CCA
4093 TGGCCA
BbvCI 227 CCTCAGC CCTCAGC(-5/-2)
2703 CCTCAGC
BclI 112 TGATCA T^GATCA
850 TGATCA
BlnI 4005 CCTAGG C^CTAGG
4966 CCTAGG
BsgI 1526 CTGCAC GTGCAG(16/14)
2946 GTGCAG
BsrFI 490 ACCGGC [GA]^CCGG[CT]
2645 ACCGGT
BstAPI 1114 GCATGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1519 GCAACCCCTGC
BstUI 2224 CGCG CG^CG
3828 CGCG
BstXI 466 CCAAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
895 CCAGCAGCTTGG
Cfr10I 490 ACCGGC [GA]^CCGG[CT]
2645 ACCGGT
EcoRI 1454 GAATTC G^AATTC
4919 GAATTC
FauI 1312 CCCGC CCCGC(4/6)
680 GCGGG
FbaI 112 TGATCA T^GATCA
850 TGATCA
HaeII 923 GGCGCT [GA]GCGC^[CT]
3483 GGCGCT
KpnI 2382 GGTACC GGTAC^C
4042 GGTACC
MluNI 1839 TGGCCA TGG^CCA
4093 TGGCCA
MscI 1839 TGGCCA TGG^CCA
4093 TGGCCA
MvnI 2224 CGCG CG^CG
3828 CGCG
NcoI 616 CCATGG C^CATGG
3295 CCATGG
NheI 2168 GCTAGC G^CTAGC
4171 GCTAGC
PshAI 1292 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3415 GACTTCTGTC
SbfI 1425 CCTGCAGG CCTGCA^GG
5091 CCTGCAGG
SphI 4827 GCATGC GCATG^C
4857 GCATGC
Sse8387I 1425 CCTGCAGG CCTGCA^GG
5091 CCTGCAGG
XmnI 141 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2811 GAAATTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII