################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AccII AclI AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AspEI AviII BbrPI BbvCI BcgI BclI BsaAI BsiEI BsiWI BsmBI BspLU11I BsrBI BsrFI BssHII BssSI BstBI BstUI Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KspI MluI MvnI NaeI NgoMIV NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XbaI XcmI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 4674 GTATAC GT^[AC][GT]AC AccIII 3223 TCCGGA T^CCGGA ApaI 1156 GGGCCC GGGCC^C AspI 2003 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 3764 TGGCCA TGG^CCA BamHI 1079 GGATCC G^GATCC BbeI 1975 GGCGCC GGCGC^C BciVI 817 GTATCC GTATCC(6/5) BlpI 2553 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2553 GCTCAGC GC^T[ACGT]AGC BsaBI 2831 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3223 TCCGGA [AT]^CCGG[AT] BseAI 3223 TCCGGA T^CCGGA BspDI 2070 ATCGAT AT^CGAT BspEI 3223 TCCGGA T^CCGGA BspHI 3279 TCATGA T^CATGA Bst1107I 4674 GTATAC GTA^TAC BstAPI 2195 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 653 GGTTACC G^GT[ACGT]ACC BstPI 653 GGTTACC G^GT[ACGT]ACC BstXI 4960 CCATTTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 4674 GTATAC GTA^TAC CelII 2553 GCTCAGC GC^T[ACGT]AGC ClaI 2070 ATCGAT AT^CGAT DraIII 1396 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 3764 TGGCCA [CT]^GGCC[GA] EcoO65I 653 GGTTACC G^GT[ACGT]ACC EcoRI 2169 GAATTC G^AATTC HaeII 1975 GGCGCC [GA]GCGC^[CT] HindIII 4681 AAGCTT A^AGCTT KasI 1975 GGCGCC G^GCGCC MamI 2831 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 359 CAATTG C^AATTG MluNI 3764 TGGCCA TGG^CCA MroI 3223 TCCGGA T^CCGGA MscI 3764 TGGCCA TGG^CCA MunI 359 CAATTG C^AATTG NarI 1975 GGCGCC GG^CGCC NdeI 4889 CATATG CA^TATG NheI 4052 GCTAGC G^CTAGC PaeR7I 440 CTCGAG C^TCGAG PflFI 2003 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2510 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3279 TCATGA T^CATGA SapI 621 GAAGAGC GCTCTTC(1/4) ScaI 3092 AGTACT AGT^ACT SfoI 1975 GGCGCC GGC^GCC SmaI 280 CCCGGG CCC^GGG Tth111I 2003 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 440 CTCGAG C^TCGAG XmaCI 280 CCCGGG C^CCGGG XmaI 280 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### Acc65I 1385 GGTACC G^GTACC 3255 GGTACC AcyI 916 GACGCC G[GA]^CG[CT]C 1975 GGCGCC AseI 3045 ATTAAT AT^TAAT 4725 ATTAAT AsnI 3045 ATTAAT AT^TAAT 4725 ATTAAT Asp718I 1385 GGTACC G^GTACC 3255 GGTACC AvrII 2548 CCTAGG C^CTAGG 3484 CCTAGG BglI 2565 GCCTAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3826 GCCAGGCTGGC BlnI 2548 CCTAGG C^CTAGG 3484 CCTAGG BsaHI 916 GACGCC G[GA]^CG[CT]C 1975 GGCGCC BsgI 1799 CTGCAC GTGCAG(16/14) 781 GTGCAG BsrDI 473 CATTGC GCAATG(2/0) 1316 CATTGC CfoI 1976 GCGC GCG^C 2030 GCGC HhaI 1976 GCGC GCG^C 2030 GCGC Hin1I 916 GACGCC G[GA]^CG[CT]C 1975 GGCGCC HinP1I 1976 GCGC G^CGC 2030 GCGC KpnI 1385 GGTACC GGTAC^C 3255 GGTACC MaeII 1582 ACGT A^CGT 3148 ACGT PpuMI 384 GGGACCT [GA]G^G[AT]CC[CT] 2515 GGGTCCT PshBI 3045 ATTAAT AT^TAAT 4725 ATTAAT StuI 436 AGGCCT AGG^CCT 3882 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AciI AcsI AfaI AflII AluI AlwI AocI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BfrI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI