##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccII AclI AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AspEI AviII BbrPI BbvCI
BcgI BclI BsaAI BsiEI BsiWI
BsmBI BspLU11I BsrBI BsrFI BssHII
BssSI BstBI BstUI Cfr10I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KspI
MluI MvnI NaeI NgoMIV NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI XcmI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 4674 GTATAC GT^[AC][GT]AC
AccIII 3223 TCCGGA T^CCGGA
ApaI 1156 GGGCCC GGGCC^C
AspI 2003 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3764 TGGCCA TGG^CCA
BamHI 1079 GGATCC G^GATCC
BbeI 1975 GGCGCC GGCGC^C
BciVI 817 GTATCC GTATCC(6/5)
BlpI 2553 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2553 GCTCAGC GC^T[ACGT]AGC
BsaBI 2831 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3223 TCCGGA [AT]^CCGG[AT]
BseAI 3223 TCCGGA T^CCGGA
BspDI 2070 ATCGAT AT^CGAT
BspEI 3223 TCCGGA T^CCGGA
BspHI 3279 TCATGA T^CATGA
Bst1107I 4674 GTATAC GTA^TAC
BstAPI 2195 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 653 GGTTACC G^GT[ACGT]ACC
BstPI 653 GGTTACC G^GT[ACGT]ACC
BstXI 4960 CCATTTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4674 GTATAC GTA^TAC
CelII 2553 GCTCAGC GC^T[ACGT]AGC
ClaI 2070 ATCGAT AT^CGAT
DraIII 1396 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 3764 TGGCCA [CT]^GGCC[GA]
EcoO65I 653 GGTTACC G^GT[ACGT]ACC
EcoRI 2169 GAATTC G^AATTC
HaeII 1975 GGCGCC [GA]GCGC^[CT]
HindIII 4681 AAGCTT A^AGCTT
KasI 1975 GGCGCC G^GCGCC
MamI 2831 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 359 CAATTG C^AATTG
MluNI 3764 TGGCCA TGG^CCA
MroI 3223 TCCGGA T^CCGGA
MscI 3764 TGGCCA TGG^CCA
MunI 359 CAATTG C^AATTG
NarI 1975 GGCGCC GG^CGCC
NdeI 4889 CATATG CA^TATG
NheI 4052 GCTAGC G^CTAGC
PaeR7I 440 CTCGAG C^TCGAG
PflFI 2003 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2510 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3279 TCATGA T^CATGA
SapI 621 GAAGAGC GCTCTTC(1/4)
ScaI 3092 AGTACT AGT^ACT
SfoI 1975 GGCGCC GGC^GCC
SmaI 280 CCCGGG CCC^GGG
Tth111I 2003 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 440 CTCGAG C^TCGAG
XmaCI 280 CCCGGG C^CCGGG
XmaI 280 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 1385 GGTACC G^GTACC
3255 GGTACC
AcyI 916 GACGCC G[GA]^CG[CT]C
1975 GGCGCC
AseI 3045 ATTAAT AT^TAAT
4725 ATTAAT
AsnI 3045 ATTAAT AT^TAAT
4725 ATTAAT
Asp718I 1385 GGTACC G^GTACC
3255 GGTACC
AvrII 2548 CCTAGG C^CTAGG
3484 CCTAGG
BglI 2565 GCCTAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3826 GCCAGGCTGGC
BlnI 2548 CCTAGG C^CTAGG
3484 CCTAGG
BsaHI 916 GACGCC G[GA]^CG[CT]C
1975 GGCGCC
BsgI 1799 CTGCAC GTGCAG(16/14)
781 GTGCAG
BsrDI 473 CATTGC GCAATG(2/0)
1316 CATTGC
CfoI 1976 GCGC GCG^C
2030 GCGC
HhaI 1976 GCGC GCG^C
2030 GCGC
Hin1I 916 GACGCC G[GA]^CG[CT]C
1975 GGCGCC
HinP1I 1976 GCGC G^CGC
2030 GCGC
KpnI 1385 GGTACC GGTAC^C
3255 GGTACC
MaeII 1582 ACGT A^CGT
3148 ACGT
PpuMI 384 GGGACCT [GA]G^G[AT]CC[CT]
2515 GGGTCCT
PshBI 3045 ATTAAT AT^TAAT
4725 ATTAAT
StuI 436 AGGCCT AGG^CCT
3882 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AocI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BfrI BglII
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI