##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccI AccII AclI AgeI
AhdI AocI Aor51HI AscI Asp718I
AspEI AviII AvrII BbeI BcgI
BclI BlnI BsaBI BsiEI BsiWI
BsmBI BspHI BspLU11I BssHII Bst1107I
BstBI BstUI BstZ17I Bsu36I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV FbaI
FseI FspI HaeII KasI KpnI
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfiI
SfoI SfuI SgrAI SnaBI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 2273 GACGTC GACGT^C
AccIII 3984 TCCGGA T^CCGGA
AflIII 3757 ACACGT A^C[GA][CT]GT
ApaI 3817 GGGCCC GGGCC^C
BamHI 3747 GGATCC G^GATCC
BbrPI 3758 CACGTG CAC^GTG
BglII 1158 AGATCT A^GATCT
BsaAI 3758 CACGTG [CT]AC^GT[GA]
BseAI 3984 TCCGGA T^CCGGA
BspDI 518 ATCGAT AT^CGAT
BspEI 3984 TCCGGA T^CCGGA
BsrFI 3735 ACCGGC [GA]^CCGG[CT]
BstEII 4595 GGTAACC G^GT[ACGT]ACC
BstPI 4595 GGTAACC G^GT[ACGT]ACC
CfoI 2750 GCGC GCG^C
Cfr10I 3735 ACCGGC [GA]^CCGG[CT]
ClaI 518 ATCGAT AT^CGAT
EcoO65I 4595 GGTAACC G^GT[ACGT]ACC
HhaI 2750 GCGC GCG^C
HinP1I 2750 GCGC G^CGC
HpaI 5262 GTTAAC GTT^AAC
MroI 3984 TCCGGA T^CCGGA
NcoI 4662 CCATGG C^CATGG
NdeI 2715 CATATG CA^TATG
PmaCI 3758 CACGTG CAC^GTG
PmlI 3758 CACGTG CAC^GTG
SbfI 4778 CCTGCAGG CCTGCA^GG
SexAI 3915 ACCTGGT A^CC[AT]GGT
SmaI 2516 CCCGGG CCC^GGG
SpeI 5256 ACTAGT A^CTAGT
SphI 6 GCATGC GCATG^C
Sse8387I 4778 CCTGCAGG CCTGCA^GG
XbaI 409 TCTAGA T^CTAGA
XmaCI 2516 CCCGGG C^CCGGG
XmaI 2516 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 1277 CTTAAG C^TTAAG
5684 CTTAAG
Alw44I 2321 GTGCAC G^TGCAC
5512 GTGCAC
ApaLI 2321 GTGCAC G^TGCAC
5512 GTGCAC
AseI 2767 ATTAAT AT^TAAT
2987 ATTAAT
AsnI 2767 ATTAAT AT^TAAT
2987 ATTAAT
AspI 1038 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1085 GACATTGTC
BanI 4148 GGTGCC G^G[CT][GA]CC
4321 GGCACC
BfrI 1277 CTTAAG C^TTAAG
5684 CTTAAG
BglI 1906 GCCTGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5082 GCCCACAAGGC
BlpI 1096 GCTGAGC GC^T[ACGT]AGC
2740 GCTCAGC
Bpu1102I 1096 GCTGAGC GC^T[ACGT]AGC
2740 GCTCAGC
BsaWI 3984 TCCGGA [AT]^CCGG[AT]
5345 ACCGGA
BsmI 2656 GAATGC GAATGC(1/-1)
4464 GAATGC
CelII 1096 GCTGAGC GC^T[ACGT]AGC
2740 GCTCAGC
DraIII 4685 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
5407 CACTATGTG
DsaI 4628 CCACGG C^C[GA][CT]GG
4662 CCATGG
EcoNI 338 CCTAAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1367 CCTGATTCAGG
EcoT22I 213 ATGCAT ATGCA^T
5736 ATGCAT
HgaI 789 GACGC GACGC(5/10)
4234 GCGTC
MslI 571 CACTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4459 CACGAGAATG
NsiI 213 ATGCAT ATGCA^T
5736 ATGCAT
PflFI 1038 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1085 GACATTGTC
PflMI 3118 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4608 CCAGGCAGTGG
PshBI 2767 ATTAAT AT^TAAT
2987 ATTAAT
SapI 704 GAAGAGC GCTCTTC(1/4)
3955 GCTCTTC
SspI 2764 AATATT AAT^ATT
5590 AATATT
Tth111I 1038 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1085 GACATTGTC
Van91I 3118 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4608 CCAGGCAGTGG
XcmI 3092 CCAAGTCAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5373 CCACTGTGGAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII Hin1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NheI
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI