##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AccI AclI AflII AhdI
ApaI AscI AspEI AvrII BciVI
BfrI BlnI BsiEI BsiWI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CpoI EagI
Eam1105I EclXI Eco52I EcoO65I FseI
HpaI KspI MluI NdeI NotI
NruI NspV PacI PaeR7I PmeI
Psp1406I PvuI RsrII SacII SalI
SfiI SfuI SgrAI SnaBI SpeI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 161 GGTACC G^GTACC
AgeI 3555 ACCGGT A^CCGGT
Alw44I 3067 GTGCAC G^TGCAC
Aor51HI 4476 AGCGCT AGC^GCT
ApaLI 3067 GTGCAC G^TGCAC
AseI 5222 ATTAAT AT^TAAT
AsnI 5222 ATTAAT AT^TAAT
Asp718I 161 GGTACC G^GTACC
BamHI 727 GGATCC G^GATCC
BbeI 793 GGCGCC GGCGC^C
BglII 920 AGATCT A^GATCT
BsaBI 876 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3249 GAGACG CGTCTC(1/5)
Bsp1407I 3168 TGTACA T^GTACA
BspHI 818 TCATGA T^CATGA
BsrDI 4880 CATTGC GCAATG(2/0)
BsrGI 3168 TGTACA T^GTACA
DraIII 4689 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4534 GACAAATTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4476 AGCGCT AGC^GCT
EcoRV 3630 GATATC GAT^ATC
EcoT22I 3826 ATGCAT ATGCA^T
HincII 2737 GTTGAC GT[CT]^[GA]AC
HindII 2737 GTTGAC GT[CT]^[GA]AC
KasI 793 GGCGCC G^GCGCC
KpnI 161 GGTACC GGTAC^C
MamI 876 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4886 CAATTG C^AATTG
MunI 4886 CAATTG C^AATTG
NaeI 4236 GCCGGC GCC^GGC
NarI 793 GGCGCC GG^CGCC
NgoMIV 4236 GCCGGC G^CCGGC
NheI 618 GCTAGC G^CTAGC
NsiI 3826 ATGCAT ATGCA^T
PinAI 3555 ACCGGT A^CCGGT
PshBI 5222 ATTAAT AT^TAAT
RcaI 818 TCATGA T^CATGA
SapI 433 GAAGAGC GCTCTTC(1/4)
SbfI 4300 CCTGCAGG CCTGCA^GG
SexAI 4431 ACCTGGT A^CC[AT]GGT
SfoI 793 GGCGCC GGC^GCC
SphI 3176 GCATGC GCATG^C
Sse8387I 4300 CCTGCAGG CCTGCA^GG
SspBI 3168 TGTACA T^GTACA
SspI 4006 AATATT AAT^ATT
XbaI 1122 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 1150 TCCGGA T^CCGGA
1724 TCCGGA
AcyI 793 GGCGCC G[GA]^CG[CT]C
4139 GGCGTC
Asp700I 1350 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3155 GAAGTGGTTC
AspI 578 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1381 GACGAGGTC
AviII 612 TGCGCA TGC^GCA
1247 TGCGCA
BbrPI 2619 CACGTG CAC^GTG
4090 CACGTG
BbvCI 1338 CCTCAGC CCTCAGC(-5/-2)
3101 GCTGAGG
BglI 3957 GCCCAGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4105 GCCTCCCTGGC
BsaAI 2619 CACGTG [CT]AC^GT[GA]
4090 CACGTG
BsaHI 793 GGCGCC G[GA]^CG[CT]C
4139 GGCGTC
BsaI 2192 GAGACC GGTCTC(1/5)
2271 GGTCTC
BseAI 1150 TCCGGA T^CCGGA
1724 TCCGGA
BsmI 1350 GAATGC GAATGC(1/-1)
4064 GCATTC
BspDI 1686 ATCGAT AT^CGAT
1847 ATCGAT
BspEI 1150 TCCGGA T^CCGGA
1724 TCCGGA
BstXI 14 CCATCGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2371 CCAGACAGGTGG
ClaI 1686 ATCGAT AT^CGAT
1847 ATCGAT
EcoNI 1736 CCTTACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5070 CCTGAAATAGG
EcoRI 3568 GAATTC G^AATTC
4031 GAATTC
FspI 612 TGCGCA TGC^GCA
1247 TGCGCA
HgaI 4140 GCGTC GACGC(5/10)
4385 GCGTC
Hin1I 793 GGCGCC G[GA]^CG[CT]C
4139 GGCGTC
HindIII 308 AAGCTT A^AGCTT
369 AAGCTT
MroI 1150 TCCGGA T^CCGGA
1724 TCCGGA
PflFI 578 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1381 GACGAGGTC
PmaCI 2619 CACGTG CAC^GTG
4090 CACGTG
PmlI 2619 CACGTG CAC^GTG
4090 CACGTG
SacI 139 GAGCTC GAGCT^C
199 GAGCTC
ScaI 773 AGTACT AGT^ACT
4901 AGTACT
SmaI 3678 CCCGGG CCC^GGG
4722 CCCGGG
Tth111I 578 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1381 GACGAGGTC
XmaCI 3678 CCCGGG C^CCGGG
4722 CCCGGG
XmaI 3678 CCCGGG C^CCGGG
4722 CCCGGG
XmnI 1350 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3155 GAAGTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII