################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## AatII AccI AclI AflII AhdI ApaI AscI AspEI AvrII BciVI BfrI BlnI BsiEI BsiWI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CpoI EagI Eam1105I EclXI Eco52I EcoO65I FseI HpaI KspI MluI NdeI NotI NruI NspV PacI PaeR7I PmeI Psp1406I PvuI RsrII SacII SalI SfiI SfuI SgrAI SnaBI SpeI SwaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 161 GGTACC G^GTACC AgeI 3555 ACCGGT A^CCGGT Alw44I 3067 GTGCAC G^TGCAC Aor51HI 4476 AGCGCT AGC^GCT ApaLI 3067 GTGCAC G^TGCAC AseI 5222 ATTAAT AT^TAAT AsnI 5222 ATTAAT AT^TAAT Asp718I 161 GGTACC G^GTACC BamHI 727 GGATCC G^GATCC BbeI 793 GGCGCC GGCGC^C BglII 920 AGATCT A^GATCT BsaBI 876 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 3249 GAGACG CGTCTC(1/5) Bsp1407I 3168 TGTACA T^GTACA BspHI 818 TCATGA T^CATGA BsrDI 4880 CATTGC GCAATG(2/0) BsrGI 3168 TGTACA T^GTACA DraIII 4689 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4534 GACAAATTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4476 AGCGCT AGC^GCT EcoRV 3630 GATATC GAT^ATC EcoT22I 3826 ATGCAT ATGCA^T HincII 2737 GTTGAC GT[CT]^[GA]AC HindII 2737 GTTGAC GT[CT]^[GA]AC KasI 793 GGCGCC G^GCGCC KpnI 161 GGTACC GGTAC^C MamI 876 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4886 CAATTG C^AATTG MunI 4886 CAATTG C^AATTG NaeI 4236 GCCGGC GCC^GGC NarI 793 GGCGCC GG^CGCC NgoMIV 4236 GCCGGC G^CCGGC NheI 618 GCTAGC G^CTAGC NsiI 3826 ATGCAT ATGCA^T PinAI 3555 ACCGGT A^CCGGT PshBI 5222 ATTAAT AT^TAAT RcaI 818 TCATGA T^CATGA SapI 433 GAAGAGC GCTCTTC(1/4) SbfI 4300 CCTGCAGG CCTGCA^GG SexAI 4431 ACCTGGT A^CC[AT]GGT SfoI 793 GGCGCC GGC^GCC SphI 3176 GCATGC GCATG^C Sse8387I 4300 CCTGCAGG CCTGCA^GG SspBI 3168 TGTACA T^GTACA SspI 4006 AATATT AAT^ATT XbaI 1122 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccIII 1150 TCCGGA T^CCGGA 1724 TCCGGA AcyI 793 GGCGCC G[GA]^CG[CT]C 4139 GGCGTC Asp700I 1350 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3155 GAAGTGGTTC AspI 578 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1381 GACGAGGTC AviII 612 TGCGCA TGC^GCA 1247 TGCGCA BbrPI 2619 CACGTG CAC^GTG 4090 CACGTG BbvCI 1338 CCTCAGC CCTCAGC(-5/-2) 3101 GCTGAGG BglI 3957 GCCCAGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4105 GCCTCCCTGGC BsaAI 2619 CACGTG [CT]AC^GT[GA] 4090 CACGTG BsaHI 793 GGCGCC G[GA]^CG[CT]C 4139 GGCGTC BsaI 2192 GAGACC GGTCTC(1/5) 2271 GGTCTC BseAI 1150 TCCGGA T^CCGGA 1724 TCCGGA BsmI 1350 GAATGC GAATGC(1/-1) 4064 GCATTC BspDI 1686 ATCGAT AT^CGAT 1847 ATCGAT BspEI 1150 TCCGGA T^CCGGA 1724 TCCGGA BstXI 14 CCATCGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2371 CCAGACAGGTGG ClaI 1686 ATCGAT AT^CGAT 1847 ATCGAT EcoNI 1736 CCTTACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5070 CCTGAAATAGG EcoRI 3568 GAATTC G^AATTC 4031 GAATTC FspI 612 TGCGCA TGC^GCA 1247 TGCGCA HgaI 4140 GCGTC GACGC(5/10) 4385 GCGTC Hin1I 793 GGCGCC G[GA]^CG[CT]C 4139 GGCGTC HindIII 308 AAGCTT A^AGCTT 369 AAGCTT MroI 1150 TCCGGA T^CCGGA 1724 TCCGGA PflFI 578 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1381 GACGAGGTC PmaCI 2619 CACGTG CAC^GTG 4090 CACGTG PmlI 2619 CACGTG CAC^GTG 4090 CACGTG SacI 139 GAGCTC GAGCT^C 199 GAGCTC ScaI 773 AGTACT AGT^ACT 4901 AGTACT SmaI 3678 CCCGGG CCC^GGG 4722 CCCGGG Tth111I 578 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1381 GACGAGGTC XmaCI 3678 CCCGGG C^CCGGG 4722 CCCGGG XmaI 3678 CCCGGG C^CCGGG 4722 CCCGGG XmnI 1350 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3155 GAAGTGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BclI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII