################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII AccIII AclI AcyI AgeI AocI ApaI AscI AspI AvrII BamHI BbeI BbrPI BcgI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BsrBI BsrDI BsrFI BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI EagI EclXI Eco52I Eco81I EcoRV FseI HgaI Hin1I KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PflFI PflMI PinAI PmaCI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I Van91I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 1014 GGTACC G^GTACC Aor51HI 3328 AGCGCT AGC^GCT Asp718I 1014 GGTACC G^GTACC AviII 847 TGCGCA TGC^GCA BciVI 3220 GGATAC GTATCC(6/5) BspHI 5441 TCATGA T^CATGA BspLU11I 4314 ACATGT A^CATGT BspMI 1648 ACCTGC ACCTGC(4/8) BssHII 1187 GCGCGC G^CGCGC BstAPI 1643 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 889 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 4681 CCGTGG C^C[GA][CT]GG Eco47III 3328 AGCGCT AGC^GCT EcoT22I 254 ATGCAT ATGCA^T FspI 847 TGCGCA TGC^GCA HpaI 3563 GTTAAC GTT^AAC KpnI 1014 GGTACC GGTAC^C NdeI 4478 CATATG CA^TATG NsiI 254 ATGCAT ATGCA^T PacI 1803 TTAATTAA TTAAT^TAA PaeR7I 1494 CTCGAG C^TCGAG PmeI 3812 GTTTAAAC GTTT^AAAC RcaI 5441 TCATGA T^CATGA SpeI 4127 ACTAGT A^CTAGT SphI 252 GCATGC GCATG^C StuI 4037 AGGCCT AGG^CCT XbaI 2208 TCTAGA T^CTAGA XhoI 1494 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 1059 CGCG CG^CG 1188 CGCG AflII 2530 CTTAAG C^TTAAG 4235 CTTAAG AflIII 3462 ACGTGT A^C[GA][CT]GT 4314 ACATGT AhdI 4944 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5064 GACCGTGAGTC Alw44I 538 GTGCAC G^TGCAC 4090 GTGCAC ApaLI 538 GTGCAC G^TGCAC 4090 GTGCAC Asp700I 1110 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2239 GAATGTCTTC AspEI 4944 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5064 GACCGTGAGTC AvaI 1494 CTCGAG C^[CT]CG[GA]G 2933 CTCGGG BalI 451 TGGCCA TGG^CCA 949 TGGCCA BcnI 1615 CCCGG CC^[GC]GG 2579 CCGGG BfrI 2530 CTTAAG C^TTAAG 4235 CTTAAG BpmI 2377 CTGGAG CTGGAG(16/14) 4720 CTGGAG BsoBI 1494 CTCGAG C^[CT]CG[GA]G 2933 CTCGGG Bsp1407I 3828 TGTACA T^GTACA 3887 TGTACA BsrGI 3828 TGTACA T^GTACA 3887 TGTACA BstEII 1704 GGTGACC G^GT[ACGT]ACC 4144 GGTGACC BstPI 1704 GGTGACC G^GT[ACGT]ACC 4144 GGTGACC BstUI 1059 CGCG CG^CG 1188 CGCG Eam1105I 4944 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5064 GACCGTGAGTC EcoO65I 1704 GGTGACC G^GT[ACGT]ACC 4144 GGTGACC EcoRI 2212 GAATTC G^AATTC 2455 GAATTC HindIII 25 AAGCTT A^AGCTT 911 AAGCTT MluNI 451 TGGCCA TGG^CCA 949 TGGCCA MscI 451 TGGCCA TGG^CCA 949 TGGCCA MvnI 1059 CGCG CG^CG 1188 CGCG NciI 1615 CCCGG CC^[GC]GG 2579 CCGGG PvuII 768 CAGCTG CAG^CTG 2732 CAGCTG SacI 870 GAGCTC GAGCT^C 1089 GAGCTC ScaI 2974 AGTACT AGT^ACT 2982 AGTACT SspBI 3828 TGTACA T^GTACA 3887 TGTACA XmnI 1110 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2239 GAATGTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaII BanI BanII BbsI BbvCI BbvI BclI BfaI BglII BlpI BmyI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DrdI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII