##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AocI ApaI AscI AspI AvrII
BamHI BbeI BbrPI BcgI BglI
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BsrBI BsrDI BsrFI BssSI
Bst1107I BstBI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI EagI EclXI
Eco52I Eco81I EcoRV FseI HgaI
Hin1I KasI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PflFI PflMI PinAI PmaCI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I Van91I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1014 GGTACC G^GTACC
Aor51HI 3328 AGCGCT AGC^GCT
Asp718I 1014 GGTACC G^GTACC
AviII 847 TGCGCA TGC^GCA
BciVI 3220 GGATAC GTATCC(6/5)
BspHI 5441 TCATGA T^CATGA
BspLU11I 4314 ACATGT A^CATGT
BspMI 1648 ACCTGC ACCTGC(4/8)
BssHII 1187 GCGCGC G^CGCGC
BstAPI 1643 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 889 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 4681 CCGTGG C^C[GA][CT]GG
Eco47III 3328 AGCGCT AGC^GCT
EcoT22I 254 ATGCAT ATGCA^T
FspI 847 TGCGCA TGC^GCA
HpaI 3563 GTTAAC GTT^AAC
KpnI 1014 GGTACC GGTAC^C
NdeI 4478 CATATG CA^TATG
NsiI 254 ATGCAT ATGCA^T
PacI 1803 TTAATTAA TTAAT^TAA
PaeR7I 1494 CTCGAG C^TCGAG
PmeI 3812 GTTTAAAC GTTT^AAAC
RcaI 5441 TCATGA T^CATGA
SpeI 4127 ACTAGT A^CTAGT
SphI 252 GCATGC GCATG^C
StuI 4037 AGGCCT AGG^CCT
XbaI 2208 TCTAGA T^CTAGA
XhoI 1494 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 1059 CGCG CG^CG
1188 CGCG
AflII 2530 CTTAAG C^TTAAG
4235 CTTAAG
AflIII 3462 ACGTGT A^C[GA][CT]GT
4314 ACATGT
AhdI 4944 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5064 GACCGTGAGTC
Alw44I 538 GTGCAC G^TGCAC
4090 GTGCAC
ApaLI 538 GTGCAC G^TGCAC
4090 GTGCAC
Asp700I 1110 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2239 GAATGTCTTC
AspEI 4944 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5064 GACCGTGAGTC
AvaI 1494 CTCGAG C^[CT]CG[GA]G
2933 CTCGGG
BalI 451 TGGCCA TGG^CCA
949 TGGCCA
BcnI 1615 CCCGG CC^[GC]GG
2579 CCGGG
BfrI 2530 CTTAAG C^TTAAG
4235 CTTAAG
BpmI 2377 CTGGAG CTGGAG(16/14)
4720 CTGGAG
BsoBI 1494 CTCGAG C^[CT]CG[GA]G
2933 CTCGGG
Bsp1407I 3828 TGTACA T^GTACA
3887 TGTACA
BsrGI 3828 TGTACA T^GTACA
3887 TGTACA
BstEII 1704 GGTGACC G^GT[ACGT]ACC
4144 GGTGACC
BstPI 1704 GGTGACC G^GT[ACGT]ACC
4144 GGTGACC
BstUI 1059 CGCG CG^CG
1188 CGCG
Eam1105I 4944 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5064 GACCGTGAGTC
EcoO65I 1704 GGTGACC G^GT[ACGT]ACC
4144 GGTGACC
EcoRI 2212 GAATTC G^AATTC
2455 GAATTC
HindIII 25 AAGCTT A^AGCTT
911 AAGCTT
MluNI 451 TGGCCA TGG^CCA
949 TGGCCA
MscI 451 TGGCCA TGG^CCA
949 TGGCCA
MvnI 1059 CGCG CG^CG
1188 CGCG
NciI 1615 CCCGG CC^[GC]GG
2579 CCGGG
PvuII 768 CAGCTG CAG^CTG
2732 CAGCTG
SacI 870 GAGCTC GAGCT^C
1089 GAGCTC
ScaI 2974 AGTACT AGT^ACT
2982 AGTACT
SspBI 3828 TGTACA T^GTACA
3887 TGTACA
XmnI 1110 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2239 GAATGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaII BanI BanII BbsI
BbvCI BbvI BclI BfaI BglII
BlpI BmyI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DrdI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII