##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccII AccIII AclI AgeI Alw44I
ApaLI AscI Asp700I AspI AviII
BbeI BglII BseAI BsiWI BspDI
BspEI BsrBI BsrFI BssHII Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco52I EcoO65I FseI FspI
KasI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NotI NruI PacI PflFI PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SnaBI Sse8387I SspI SwaI
Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 185 GACGTC GACGT^C
Acc65I 2052 GGTACC G^GTACC
AccI 4105 GTCTAC GT^[AC][GT]AC
AflII 4973 CTTAAG C^TTAAG
AflIII 92 ACATGT A^C[GA][CT]GT
Aor51HI 2535 AGCGCT AGC^GCT
Asp718I 2052 GGTACC G^GTACC
AvrII 4512 CCTAGG C^CTAGG
BalI 2803 TGGCCA TGG^CCA
BbrPI 6273 CACGTG CAC^GTG
BclI 832 TGATCA T^GATCA
BfrI 4973 CTTAAG C^TTAAG
BlnI 4512 CCTAGG C^CTAGG
BsaAI 6273 CACGTG [CT]AC^GT[GA]
BsaBI 4694 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2030 CGACCG CG[GA][CT]^CG
BsmBI 3582 CGTCTC CGTCTC(1/5)
BspLU11I 92 ACATGT A^CATGT
BssSI 1020 CTCGTG CACGAG(-5/-1)
BstBI 3741 TTCGAA TT^CGAA
EaeI 2803 TGGCCA [CT]^GGCC[GA]
Eco47III 2535 AGCGCT AGC^GCT
EcoNI 3251 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2233 GATATC GAT^ATC
FbaI 832 TGATCA T^GATCA
HgaI 2488 GCGTC GACGC(5/10)
HindIII 846 AAGCTT A^AGCTT
HpaI 4757 GTTAAC GTT^AAC
KpnI 2052 GGTACC GGTAC^C
MamI 4694 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2803 TGGCCA TGG^CCA
MscI 2803 TGGCCA TGG^CCA
NheI 4220 GCTAGC G^CTAGC
NspV 3741 TTCGAA TT^CGAA
PmaCI 6273 CACGTG CAC^GTG
PmlI 6273 CACGTG CAC^GTG
ScaI 6069 AGTACT AGT^ACT
SfuI 3741 TTCGAA TT^CGAA
SpeI 2630 ACTAGT A^CTAGT
StuI 4389 AGGCCT AGG^CCT
XbaI 2021 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 185 GACGTC G[GA]^CG[CT]C
2487 GGCGTC
AhdI 4348 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4657 GACTTGTGGTC
AocI 1045 CCTGAGG CC^T[ACGT]AGG
3731 CCTAAGG
ApaI 44 GGGCCC GGGCC^C
4237 GGGCCC
AspEI 4348 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4657 GACTTGTGGTC
BbvCI 3540 CCTCAGC CCTCAGC(-5/-2)
100 GCTGAGG
BcgI 340 GCATGGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2111 GCAGAACTGTCG
BglI 2479 GCCTGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4382 GCCTACCAGGC
BlpI 2012 GCTAAGC GC^T[ACGT]AGC
3852 GCTCAGC
Bpu1102I 2012 GCTAAGC GC^T[ACGT]AGC
3852 GCTCAGC
BsaHI 185 GACGTC G[GA]^CG[CT]C
2487 GGCGTC
Bsu36I 1045 CCTGAGG CC^T[ACGT]AGG
3731 CCTAAGG
CelII 2012 GCTAAGC GC^T[ACGT]AGC
3852 GCTCAGC
DraI 1142 TTTAAA TTT^AAA
5670 TTTAAA
Eam1105I 4348 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4657 GACTTGTGGTC
Eco81I 1045 CCTGAGG CC^T[ACGT]AGG
3731 CCTAAGG
EcoRI 757 GAATTC G^AATTC
3430 GAATTC
EcoT22I 1745 ATGCAT ATGCA^T
5034 ATGCAT
HaeII 60 AGCGCC [GA]GCGC^[CT]
2535 AGCGCT
Hin1I 185 GACGTC G[GA]^CG[CT]C
2487 GGCGTC
NcoI 3523 CCATGG C^CATGG
5764 CCATGG
NdeI 5679 CATATG CA^TATG
5928 CATATG
NsiI 1745 ATGCAT ATGCA^T
5034 ATGCAT
PpuMI 1955 AGGTCCT [GA]G^G[AT]CC[CT]
3332 AGGACCT
SacI 3850 GAGCTC GAGCT^C
5160 GAGCTC
SapI 298 GAAGAGC GCTCTTC(1/4)
1165 GCTCTTC
SmaI 18 CCCGGG CCC^GGG
41 CCCGGG
SphI 1747 GCATGC GCATG^C
5394 GCATGC
XmaCI 18 CCCGGG C^CCGGG
41 CCCGGG
XmaI 18 CCCGGG C^CCGGG
41 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PaeR7I PflMI PleI PshBI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII