################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AccII AccIII AclI AgeI Alw44I ApaLI AscI Asp700I AspI AviII BbeI BglII BseAI BsiWI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DrdI EagI EclXI Eco52I EcoO65I FseI FspI KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NotI NruI PacI PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SnaBI Sse8387I SspI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 185 GACGTC GACGT^C Acc65I 2052 GGTACC G^GTACC AccI 4105 GTCTAC GT^[AC][GT]AC AflII 4973 CTTAAG C^TTAAG AflIII 92 ACATGT A^C[GA][CT]GT Aor51HI 2535 AGCGCT AGC^GCT Asp718I 2052 GGTACC G^GTACC AvrII 4512 CCTAGG C^CTAGG BalI 2803 TGGCCA TGG^CCA BbrPI 6273 CACGTG CAC^GTG BclI 832 TGATCA T^GATCA BfrI 4973 CTTAAG C^TTAAG BlnI 4512 CCTAGG C^CTAGG BsaAI 6273 CACGTG [CT]AC^GT[GA] BsaBI 4694 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 2030 CGACCG CG[GA][CT]^CG BsmBI 3582 CGTCTC CGTCTC(1/5) BspLU11I 92 ACATGT A^CATGT BssSI 1020 CTCGTG CACGAG(-5/-1) BstBI 3741 TTCGAA TT^CGAA EaeI 2803 TGGCCA [CT]^GGCC[GA] Eco47III 2535 AGCGCT AGC^GCT EcoNI 3251 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 2233 GATATC GAT^ATC FbaI 832 TGATCA T^GATCA HgaI 2488 GCGTC GACGC(5/10) HindIII 846 AAGCTT A^AGCTT HpaI 4757 GTTAAC GTT^AAC KpnI 2052 GGTACC GGTAC^C MamI 4694 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2803 TGGCCA TGG^CCA MscI 2803 TGGCCA TGG^CCA NheI 4220 GCTAGC G^CTAGC NspV 3741 TTCGAA TT^CGAA PmaCI 6273 CACGTG CAC^GTG PmlI 6273 CACGTG CAC^GTG ScaI 6069 AGTACT AGT^ACT SfuI 3741 TTCGAA TT^CGAA SpeI 2630 ACTAGT A^CTAGT StuI 4389 AGGCCT AGG^CCT XbaI 2021 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 185 GACGTC G[GA]^CG[CT]C 2487 GGCGTC AhdI 4348 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4657 GACTTGTGGTC AocI 1045 CCTGAGG CC^T[ACGT]AGG 3731 CCTAAGG ApaI 44 GGGCCC GGGCC^C 4237 GGGCCC AspEI 4348 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4657 GACTTGTGGTC BbvCI 3540 CCTCAGC CCTCAGC(-5/-2) 100 GCTGAGG BcgI 340 GCATGGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2111 GCAGAACTGTCG BglI 2479 GCCTGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4382 GCCTACCAGGC BlpI 2012 GCTAAGC GC^T[ACGT]AGC 3852 GCTCAGC Bpu1102I 2012 GCTAAGC GC^T[ACGT]AGC 3852 GCTCAGC BsaHI 185 GACGTC G[GA]^CG[CT]C 2487 GGCGTC Bsu36I 1045 CCTGAGG CC^T[ACGT]AGG 3731 CCTAAGG CelII 2012 GCTAAGC GC^T[ACGT]AGC 3852 GCTCAGC DraI 1142 TTTAAA TTT^AAA 5670 TTTAAA Eam1105I 4348 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4657 GACTTGTGGTC Eco81I 1045 CCTGAGG CC^T[ACGT]AGG 3731 CCTAAGG EcoRI 757 GAATTC G^AATTC 3430 GAATTC EcoT22I 1745 ATGCAT ATGCA^T 5034 ATGCAT HaeII 60 AGCGCC [GA]GCGC^[CT] 2535 AGCGCT Hin1I 185 GACGTC G[GA]^CG[CT]C 2487 GGCGTC NcoI 3523 CCATGG C^CATGG 5764 CCATGG NdeI 5679 CATATG CA^TATG 5928 CATATG NsiI 1745 ATGCAT ATGCA^T 5034 ATGCAT PpuMI 1955 AGGTCCT [GA]G^G[AT]CC[CT] 3332 AGGACCT SacI 3850 GAGCTC GAGCT^C 5160 GAGCTC SapI 298 GAAGAGC GCTCTTC(1/4) 1165 GCTCTTC SmaI 18 CCCGGG CCC^GGG 41 CCCGGG SphI 1747 GCATGC GCATG^C 5394 GCATGC XmaCI 18 CCCGGG C^CCGGG 41 CCCGGG XmaI 18 CCCGGG C^CCGGG 41 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaI AvaII BamHI BanI BanII BbsI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PaeR7I PflMI PleI PshBI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII