##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
Alw44I ApaLI AscI AseI AsnI
AviII BalI BamHI BclI BfrI
BglII BsaBI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BssHII BstBI ClaI
CpoI EagI EclXI Eco52I EcoRI
EcoT22I FbaI FseI FspI MamI
MfeI MluI MluNI MroI MscI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII ScaI SfiI SfuI SgrAI
SnaBI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 1858 CGCG CG^CG
AhdI 1798 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3011 AGCGCT AGC^GCT
Asp700I 5272 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1798 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 3433 GGCGCC GGCGC^C
BbrPI 848 CACGTG CAC^GTG
BbvCI 593 GCTGAGG CCTCAGC(-5/-2)
BcgI 4251 GCAGGGAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4633 GTATCC GTATCC(6/5)
BsaWI 3219 ACCGGA [AT]^CCGG[AT]
Bsp1407I 919 TGTACA T^GTACA
BsrGI 919 TGTACA T^GTACA
Bst1107I 3107 GTATAC GTA^TAC
BstAPI 1614 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1858 CGCG CG^CG
BstZ17I 3107 GTATAC GTA^TAC
DraI 4734 TTTAAA TTT^AAA
DraIII 995 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1628 GACACCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1798 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3011 AGCGCT AGC^GCT
EcoNI 1596 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4021 GATATC GAT^ATC
KasI 3433 GGCGCC G^GCGCC
KspI 1857 CCGCGG CCGC^GG
MvnI 1858 CGCG CG^CG
NarI 3433 GGCGCC GG^CGCC
PmaCI 848 CACGTG CAC^GTG
PmlI 848 CACGTG CAC^GTG
SacII 1857 CCGCGG CCGC^GG
SalI 699 GTCGAC G^TCGAC
SbfI 764 CCTGCAGG CCTGCA^GG
SfoI 3433 GGCGCC GGC^GCC
SphI 2866 GCATGC GCATG^C
Sse8387I 764 CCTGCAGG CCTGCA^GG
SspBI 919 TGTACA T^GTACA
XmnI 5272 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 95 GACGTC GACGT^C
2945 GACGTC
Acc65I 496 GGTACC G^GTACC
1605 GGTACC
Asp718I 496 GGTACC G^GTACC
1605 GGTACC
AvrII 3894 CCTAGG C^CTAGG
4712 CCTAGG
BlnI 3894 CCTAGG C^CTAGG
4712 CCTAGG
BsaAI 848 CACGTG [CT]AC^GT[GA]
884 TACGTG
BsaI 2500 GAGACC GGTCTC(1/5)
2633 GAGACC
BstEII 520 GGTCACC G^GT[ACGT]ACC
3215 GGTGACC
BstPI 520 GGTCACC G^GT[ACGT]ACC
3215 GGTGACC
EcoO65I 520 GGTCACC G^GT[ACGT]ACC
3215 GGTGACC
HindIII 655 AAGCTT A^AGCTT
3027 AAGCTT
HpaI 4766 GTTAAC GTT^AAC
5197 GTTAAC
KpnI 496 GGTACC GGTAC^C
1605 GGTACC
NcoI 516 CCATGG C^CATGG
1675 CCATGG
NspI 823 GCATGT [GA]CATG^[CT]
2866 GCATGC
PshAI 702 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2375 GACCTGCGTC
SacI 432 GAGCTC GAGCT^C
3667 GAGCTC
SapI 3774 GAAGAGC GCTCTTC(1/4)
2283 GCTCTTC
SexAI 1281 ACCTGGT A^CC[AT]GGT
4385 ACCAGGT
SpeI 1353 ACTAGT A^CTAGT
4762 ACTAGT
StuI 2706 AGGCCT AGG^CCT
4000 AGGCCT
XcmI 4114 CCATGCCTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4708 CCAGCCTAGGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AspI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII XmaCI XmaI