##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccII AclI AflII AgeI
AhdI AscI AseI AsnI AspEI
AviII BciVI BfrI BsiWI BspDI
BssHII Bst1107I BstUI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV FseI
FspI HindIII HpaI KspI MfeI
MluI MunI MvnI NaeI NgoMIV
NotI NruI PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SexAI
SgrAI SmaI SnaBI SpeI SspI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 1159 GGTACC G^GTACC
AccIII 64 TCCGGA T^CCGGA
Asp718I 1159 GGTACC G^GTACC
AvrII 1377 CCTAGG C^CTAGG
BlnI 1377 CCTAGG C^CTAGG
BsaBI 5260 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 64 TCCGGA T^CCGGA
BsiEI 3320 CGACCG CG[GA][CT]^CG
Bsp1407I 2338 TGTACA T^GTACA
BspEI 64 TCCGGA T^CCGGA
BsrGI 2338 TGTACA T^GTACA
BstBI 5683 TTCGAA TT^CGAA
KpnI 1159 GGTACC GGTAC^C
MamI 5260 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 64 TCCGGA T^CCGGA
NdeI 4804 CATATG CA^TATG
NspV 5683 TTCGAA TT^CGAA
SfuI 5683 TTCGAA TT^CGAA
SspBI 2338 TGTACA T^GTACA
XcmI 235 CCAACAGTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 3060 GTCTAC GT^[AC][GT]AC
4140 GTAGAC
AocI 1714 CCTTAGG CC^T[ACGT]AGG
4271 CCTAAGG
Aor51HI 105 AGCGCT AGC^GCT
1910 AGCGCT
AspI 2190 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5946 GACTCTGTC
AvaI 461 CCCGAG C^[CT]CG[GA]G
3861 CCCGAG
BbeI 1849 GGCGCC GGCGC^C
4866 GGCGCC
BlpI 3329 GCTGAGC GC^T[ACGT]AGC
5321 GCTTAGC
Bpu1102I 3329 GCTGAGC GC^T[ACGT]AGC
5321 GCTTAGC
BsaWI 64 TCCGGA [AT]^CCGG[AT]
3322 ACCGGA
BsmI 872 GAATGC GAATGC(1/-1)
4073 GAATGC
BsoBI 461 CCCGAG C^[CT]CG[GA]G
3861 CCCGAG
BspHI 361 TCATGA T^CATGA
2923 TCATGA
BspLU11I 918 ACATGT A^CATGT
2335 ACATGT
BstEII 1436 GGTCACC G^GT[ACGT]ACC
2981 GGTCACC
BstPI 1436 GGTCACC G^GT[ACGT]ACC
2981 GGTCACC
Bsu36I 1714 CCTTAGG CC^T[ACGT]AGG
4271 CCTAAGG
CelII 3329 GCTGAGC GC^T[ACGT]AGC
5321 GCTTAGC
Eco47III 105 AGCGCT AGC^GCT
1910 AGCGCT
Eco81I 1714 CCTTAGG CC^T[ACGT]AGG
4271 CCTAAGG
EcoNI 2720 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4628 CCTGCAGCAGG
EcoO65I 1436 GGTCACC G^GT[ACGT]ACC
2981 GGTCACC
EcoT22I 885 ATGCAT ATGCA^T
4057 ATGCAT
HincII 11 GTTGAC GT[CT]^[GA]AC
4503 GTCAAC
HindII 11 GTTGAC GT[CT]^[GA]AC
4503 GTCAAC
KasI 1849 GGCGCC G^GCGCC
4866 GGCGCC
MseI 2734 TTAA T^TAA
4129 TTAA
NarI 1849 GGCGCC GG^CGCC
4866 GGCGCC
NsiI 885 ATGCAT ATGCA^T
4057 ATGCAT
PflFI 2190 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5946 GACTCTGTC
RcaI 361 TCATGA T^CATGA
2923 TCATGA
SbfI 521 CCTGCAGG CCTGCA^GG
3311 CCTGCAGG
SfoI 1849 GGCGCC GGC^GCC
4866 GGCGCC
SmlI 2225 CTTGAG C^T[CT][GA]AG
5358 CTCAAG
SphI 3181 GCATGC GCATG^C
3668 GCATGC
Sse8387I 521 CCTGCAGG CCTGCA^GG
3311 CCTGCAGG
Tru9I 2734 TTAA T^TAA
4129 TTAA
Tth111I 2190 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5946 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI Asp700I AspHI AvaII
BalI BamHI BanI BanII BbrPI
BbsI BbvCI BbvI BcgI BclI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NheI
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI