################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AccII AclI AflII AgeI AhdI AscI AseI AsnI AspEI AviII BciVI BfrI BsiWI BspDI BssHII Bst1107I BstUI BstZ17I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoRI EcoRV FseI FspI HindIII HpaI KspI MfeI MluI MunI MvnI NaeI NgoMIV NotI NruI PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SgrAI SmaI SnaBI SpeI SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 20 enzymes) # ################################### Acc65I 1159 GGTACC G^GTACC AccIII 64 TCCGGA T^CCGGA Asp718I 1159 GGTACC G^GTACC AvrII 1377 CCTAGG C^CTAGG BlnI 1377 CCTAGG C^CTAGG BsaBI 5260 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 64 TCCGGA T^CCGGA BsiEI 3320 CGACCG CG[GA][CT]^CG Bsp1407I 2338 TGTACA T^GTACA BspEI 64 TCCGGA T^CCGGA BsrGI 2338 TGTACA T^GTACA BstBI 5683 TTCGAA TT^CGAA KpnI 1159 GGTACC GGTAC^C MamI 5260 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 64 TCCGGA T^CCGGA NdeI 4804 CATATG CA^TATG NspV 5683 TTCGAA TT^CGAA SfuI 5683 TTCGAA TT^CGAA SspBI 2338 TGTACA T^GTACA XcmI 235 CCAACAGTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccI 3060 GTCTAC GT^[AC][GT]AC 4140 GTAGAC AocI 1714 CCTTAGG CC^T[ACGT]AGG 4271 CCTAAGG Aor51HI 105 AGCGCT AGC^GCT 1910 AGCGCT AspI 2190 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 5946 GACTCTGTC AvaI 461 CCCGAG C^[CT]CG[GA]G 3861 CCCGAG BbeI 1849 GGCGCC GGCGC^C 4866 GGCGCC BlpI 3329 GCTGAGC GC^T[ACGT]AGC 5321 GCTTAGC Bpu1102I 3329 GCTGAGC GC^T[ACGT]AGC 5321 GCTTAGC BsaWI 64 TCCGGA [AT]^CCGG[AT] 3322 ACCGGA BsmI 872 GAATGC GAATGC(1/-1) 4073 GAATGC BsoBI 461 CCCGAG C^[CT]CG[GA]G 3861 CCCGAG BspHI 361 TCATGA T^CATGA 2923 TCATGA BspLU11I 918 ACATGT A^CATGT 2335 ACATGT BstEII 1436 GGTCACC G^GT[ACGT]ACC 2981 GGTCACC BstPI 1436 GGTCACC G^GT[ACGT]ACC 2981 GGTCACC Bsu36I 1714 CCTTAGG CC^T[ACGT]AGG 4271 CCTAAGG CelII 3329 GCTGAGC GC^T[ACGT]AGC 5321 GCTTAGC Eco47III 105 AGCGCT AGC^GCT 1910 AGCGCT Eco81I 1714 CCTTAGG CC^T[ACGT]AGG 4271 CCTAAGG EcoNI 2720 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4628 CCTGCAGCAGG EcoO65I 1436 GGTCACC G^GT[ACGT]ACC 2981 GGTCACC EcoT22I 885 ATGCAT ATGCA^T 4057 ATGCAT HincII 11 GTTGAC GT[CT]^[GA]AC 4503 GTCAAC HindII 11 GTTGAC GT[CT]^[GA]AC 4503 GTCAAC KasI 1849 GGCGCC G^GCGCC 4866 GGCGCC MseI 2734 TTAA T^TAA 4129 TTAA NarI 1849 GGCGCC GG^CGCC 4866 GGCGCC NsiI 885 ATGCAT ATGCA^T 4057 ATGCAT PflFI 2190 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 5946 GACTCTGTC RcaI 361 TCATGA T^CATGA 2923 TCATGA SbfI 521 CCTGCAGG CCTGCA^GG 3311 CCTGCAGG SfoI 1849 GGCGCC GGC^GCC 4866 GGCGCC SmlI 2225 CTTGAG C^T[CT][GA]AG 5358 CTCAAG SphI 3181 GCATGC GCATG^C 3668 GCATGC Sse8387I 521 CCTGCAGG CCTGCA^GG 3311 CCTGCAGG Tru9I 2734 TTAA T^TAA 4129 TTAA Tth111I 2190 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 5946 GACTCTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI Asp700I AspHI AvaII BalI BamHI BanI BanII BbrPI BbsI BbvCI BbvI BcgI BclI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI