################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AatII AccI AccIII AclI AgeI AhdI AscI AseI AsnI AspEI BamHI BbrPI BclI BglII BseAI BsiWI BspDI BspEI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI Eam1105I EcoO65I FbaI FseI HpaI MroI NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfuI SgrAI SphI Sse8387I StuI SwaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Aor51HI 180 AGCGCT AGC^GCT Asp700I 1879 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1748 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 1951 CGAGGTGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 2715 GCTTAGC GC^T[ACGT]AGC Bpu1102I 2715 GCTTAGC GC^T[ACGT]AGC BsaWI 5179 TCCGGT [AT]^CCGG[AT] BsiEI 2039 CGGCCG CG[GA][CT]^CG BsmBI 776 GAGACG CGTCTC(1/5) BstYI 357 AGATCC [GA]^GATC[CT] CelII 2715 GCTTAGC GC^T[ACGT]AGC DraIII 3824 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2223 GACCCACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2039 CGGCCG C^GGCCG EclXI 2039 CGGCCG C^GGCCG Eco47III 180 AGCGCT AGC^GCT Eco52I 2039 CGGCCG C^GGCCG HindIII 4819 AAGCTT A^AGCTT KspI 2069 CCGCGG CCGC^GG MfeI 3242 CAATTG C^AATTG MflI 357 AGATCC [GA]^GATC[CT] MunI 3242 CAATTG C^AATTG NcoI 4764 CCATGG C^CATGG NdeI 6106 CATATG CA^TATG PaeR7I 1382 CTCGAG C^TCGAG PflFI 1748 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC SacII 2069 CCGCGG CCGC^GG SmaI 770 CCCGGG CCC^GGG SnaBI 3173 TACGTA TAC^GTA SpeI 4162 ACTAGT A^CTAGT Tth111I 1748 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1382 CTCGAG C^TCGAG XhoII 357 AGATCC [GA]^GATC[CT] XmaCI 770 CCCGGG C^CCGGG XmaI 770 CCCGGG C^CCGGG XmnI 1879 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 358 GATCC GGATC(4/5) 2294 GGATC AocI 2189 CCTGAGG CC^T[ACGT]AGG 2573 CCTCAGG ApaI 2173 GGGCCC GGGCC^C 5474 GGGCCC AviII 510 TGCGCA TGC^GCA 792 TGCGCA AvrII 2263 CCTAGG C^CTAGG 2504 CCTAGG BciVI 3226 GGATAC GTATCC(6/5) 3261 GGATAC BlnI 2263 CCTAGG C^CTAGG 2504 CCTAGG BsaBI 409 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4987 GATGATAATC BsrBI 2094 CCGCTC CCGCTC(-3/-3) 1553 GAGCGG Bsu36I 2189 CCTGAGG CC^T[ACGT]AGG 2573 CCTCAGG Eco81I 2189 CCTGAGG CC^T[ACGT]AGG 2573 CCTCAGG EcoNI 2366 CCTTTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5530 CCTAATGTAGG FspI 510 TGCGCA TGC^GCA 792 TGCGCA HincII 1754 GTCAAC GT[CT]^[GA]AC 4829 GTTGAC HindII 1754 GTCAAC GT[CT]^[GA]AC 4829 GTTGAC MamI 409 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4987 GATGATAATC MluI 1842 ACGCGT A^CGCGT 4538 ACGCGT NaeI 339 GCCGGC GCC^GGC 2037 GCCGGC NgoMIV 339 GCCGGC G^CCGGC 2037 GCCGGC SacI 2580 GAGCTC GAGCT^C 5239 GAGCTC SspI 4946 AATATT AAT^ATT 5379 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 144 enzymes) # ######################################### Acc65I AccII AciI AcsI AcyI AfaI AflII AflIII AluI Alw44I AlwNI ApaLI ApoI Asp718I AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BfrI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SfoI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI