##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AhdI AscI AseI AsnI AspEI
BamHI BbrPI BclI BglII BseAI
BsiWI BspDI BspEI BspLU11I BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI Eam1105I EcoO65I FbaI
FseI HpaI MroI NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfiI SfuI SgrAI SphI Sse8387I
StuI SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Aor51HI 180 AGCGCT AGC^GCT
Asp700I 1879 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1748 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1951 CGAGGTGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2715 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2715 GCTTAGC GC^T[ACGT]AGC
BsaWI 5179 TCCGGT [AT]^CCGG[AT]
BsiEI 2039 CGGCCG CG[GA][CT]^CG
BsmBI 776 GAGACG CGTCTC(1/5)
BstYI 357 AGATCC [GA]^GATC[CT]
CelII 2715 GCTTAGC GC^T[ACGT]AGC
DraIII 3824 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2223 GACCCACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2039 CGGCCG C^GGCCG
EclXI 2039 CGGCCG C^GGCCG
Eco47III 180 AGCGCT AGC^GCT
Eco52I 2039 CGGCCG C^GGCCG
HindIII 4819 AAGCTT A^AGCTT
KspI 2069 CCGCGG CCGC^GG
MfeI 3242 CAATTG C^AATTG
MflI 357 AGATCC [GA]^GATC[CT]
MunI 3242 CAATTG C^AATTG
NcoI 4764 CCATGG C^CATGG
NdeI 6106 CATATG CA^TATG
PaeR7I 1382 CTCGAG C^TCGAG
PflFI 1748 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 2069 CCGCGG CCGC^GG
SmaI 770 CCCGGG CCC^GGG
SnaBI 3173 TACGTA TAC^GTA
SpeI 4162 ACTAGT A^CTAGT
Tth111I 1748 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1382 CTCGAG C^TCGAG
XhoII 357 AGATCC [GA]^GATC[CT]
XmaCI 770 CCCGGG C^CCGGG
XmaI 770 CCCGGG C^CCGGG
XmnI 1879 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 358 GATCC GGATC(4/5)
2294 GGATC
AocI 2189 CCTGAGG CC^T[ACGT]AGG
2573 CCTCAGG
ApaI 2173 GGGCCC GGGCC^C
5474 GGGCCC
AviII 510 TGCGCA TGC^GCA
792 TGCGCA
AvrII 2263 CCTAGG C^CTAGG
2504 CCTAGG
BciVI 3226 GGATAC GTATCC(6/5)
3261 GGATAC
BlnI 2263 CCTAGG C^CTAGG
2504 CCTAGG
BsaBI 409 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4987 GATGATAATC
BsrBI 2094 CCGCTC CCGCTC(-3/-3)
1553 GAGCGG
Bsu36I 2189 CCTGAGG CC^T[ACGT]AGG
2573 CCTCAGG
Eco81I 2189 CCTGAGG CC^T[ACGT]AGG
2573 CCTCAGG
EcoNI 2366 CCTTTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5530 CCTAATGTAGG
FspI 510 TGCGCA TGC^GCA
792 TGCGCA
HincII 1754 GTCAAC GT[CT]^[GA]AC
4829 GTTGAC
HindII 1754 GTCAAC GT[CT]^[GA]AC
4829 GTTGAC
MamI 409 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4987 GATGATAATC
MluI 1842 ACGCGT A^CGCGT
4538 ACGCGT
NaeI 339 GCCGGC GCC^GGC
2037 GCCGGC
NgoMIV 339 GCCGGC G^CCGGC
2037 GCCGGC
SacI 2580 GAGCTC GAGCT^C
5239 GAGCTC
SspI 4946 AATATT AAT^ATT
5379 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflII AflIII AluI Alw44I
AlwNI ApaLI ApoI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BfrI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrFI
BsrGI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SfoI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI