################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AccIII AgeI AhdI Aor51HI AscI AspEI AviII BalI BbeI BbrPI BciVI BsaAI BseAI BsiEI BsiWI BspDI BspEI BsrDI BssHII BssSI BstAPI BstXI ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FseI FspI HindIII KasI MluNI MroI MscI NarI NotI NruI PacI PaeR7I PinAI PmaCI PmlI PshAI PvuI RsrII SalI SapI SbfI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AclI 1463 AACGTT AA^CGTT Alw44I 2632 GTGCAC G^TGCAC ApaI 4607 GGGCCC GGGCC^C ApaLI 2632 GTGCAC G^TGCAC AseI 2102 ATTAAT AT^TAAT AsnI 2102 ATTAAT AT^TAAT AspI 1512 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3232 CCTAGG C^CTAGG BcgI 305 GCATCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2175 TGATCA T^GATCA BglII 833 AGATCT A^GATCT BlnI 3232 CCTAGG C^CTAGG BsaBI 4484 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 463 GAGACC GGTCTC(1/5) BsaWI 4454 TCCGGT [AT]^CCGG[AT] BsmI 3900 GAATGC GAATGC(1/-1) BspLU11I 4625 ACATGT A^CATGT BspMI 3549 GCAGGT ACCTGC(4/8) Bst1107I 1726 GTATAC GTA^TAC BstBI 793 TTCGAA TT^CGAA BstEII 1484 GGTAACC G^GT[ACGT]ACC BstPI 1484 GGTAACC G^GT[ACGT]ACC BstZ17I 1726 GTATAC GTA^TAC EaeI 2721 TGGCCG [CT]^GGCC[GA] EcoO65I 1484 GGTAACC G^GT[ACGT]ACC EcoT22I 2322 ATGCAT ATGCA^T FbaI 2175 TGATCA T^GATCA HincII 3915 GTTAAC GT[CT]^[GA]AC HindII 3915 GTTAAC GT[CT]^[GA]AC HpaI 3915 GTTAAC GTT^AAC KspI 35 CCGCGG CCGC^GG MamI 4484 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2150 CAATTG C^AATTG MluI 1918 ACGCGT A^CGCGT MunI 2150 CAATTG C^AATTG NcoI 1814 CCATGG C^CATGG NdeI 3683 CATATG CA^TATG NheI 1637 GCTAGC G^CTAGC NsiI 2322 ATGCAT ATGCA^T NspV 793 TTCGAA TT^CGAA PflFI 1512 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmeI 2195 GTTTAAAC GTTT^AAAC PshBI 2102 ATTAAT AT^TAAT Psp1406I 1463 AACGTT AA^CGTT SacII 35 CCGCGG CCGC^GG ScaI 338 AGTACT AGT^ACT SexAI 713 ACCTGGT A^CC[AT]GGT SfiI 2722 GGCCGAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 793 TTCGAA TT^CGAA SspI 2114 AATATT AAT^ATT Tth111I 1512 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AatII 1066 GACGTC GACGT^C 1865 GACGTC Acc65I 1809 GGTACC G^GTACC 3927 GGTACC AccI 1726 GTATAC GT^[AC][GT]AC 4092 GTCTAC AcyI 1066 GACGTC G[GA]^CG[CT]C 1865 GACGTC AflII 586 CTTAAG C^TTAAG 3707 CTTAAG AflIII 1918 ACGCGT A^C[GA][CT]GT 4625 ACATGT AocI 1350 CCTGAGG CC^T[ACGT]AGG 2262 CCTCAGG Asp700I 1447 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3579 GAACATTTTC Asp718I 1809 GGTACC G^GTACC 3927 GGTACC AvaI 190 CCCGAG C^[CT]CG[GA]G 214 CCCGAG BamHI 269 GGATCC G^GATCC 630 GGATCC BbvCI 1143 GCTGAGG CCTCAGC(-5/-2) 1761 GCTGAGG BfrI 586 CTTAAG C^TTAAG 3707 CTTAAG BglI 2723 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4276 GCCCATCTGGC BlpI 1980 GCTGAGC GC^T[ACGT]AGC 2883 GCTTAGC Bpu1102I 1980 GCTGAGC GC^T[ACGT]AGC 2883 GCTTAGC BsaHI 1066 GACGTC G[GA]^CG[CT]C 1865 GACGTC BsoBI 190 CCCGAG C^[CT]CG[GA]G 214 CCCGAG Bsp1407I 740 TGTACA T^GTACA 1529 TGTACA BspHI 1831 TCATGA T^CATGA 2178 TCATGA BsrFI 178 GCCGGC [GA]^CCGG[CT] 550 GCCGGC BsrGI 740 TGTACA T^GTACA 1529 TGTACA Bsu36I 1350 CCTGAGG CC^T[ACGT]AGG 2262 CCTCAGG CelII 1980 GCTGAGC GC^T[ACGT]AGC 2883 GCTTAGC Cfr10I 178 GCCGGC [GA]^CCGG[CT] 550 GCCGGC Eco81I 1350 CCTGAGG CC^T[ACGT]AGG 2262 CCTCAGG EcoNI 1350 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4313 CCTTACCAAGG Hin1I 1066 GACGTC G[GA]^CG[CT]C 1865 GACGTC KpnI 1809 GGTACC GGTAC^C 3927 GGTACC NaeI 178 GCCGGC GCC^GGC 550 GCCGGC NgoMIV 178 GCCGGC G^CCGGC 550 GCCGGC PpuMI 209 AGGTCCC [GA]G^G[AT]CC[CT] 317 GGGACCC RcaI 1831 TCATGA T^CATGA 2178 TCATGA SacI 1983 GAGCTC GAGCT^C 2959 GAGCTC SphI 1365 GCATGC GCATG^C 1522 GCATGC SspBI 740 TGTACA T^GTACA 1529 TGTACA StuI 218 AGGCCT AGG^CCT 473 AGGCCT XbaI 3779 TCTAGA T^CTAGA 4480 TCTAGA XmnI 1447 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3579 GAACATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII