##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccIII AgeI AhdI Aor51HI AscI
AspEI AviII BalI BbeI BbrPI
BciVI BsaAI BseAI BsiEI BsiWI
BspDI BspEI BsrDI BssHII BssSI
BstAPI BstXI ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV FseI FspI HindIII
KasI MluNI MroI MscI NarI
NotI NruI PacI PaeR7I PinAI
PmaCI PmlI PshAI PvuI RsrII
SalI SapI SbfI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AclI 1463 AACGTT AA^CGTT
Alw44I 2632 GTGCAC G^TGCAC
ApaI 4607 GGGCCC GGGCC^C
ApaLI 2632 GTGCAC G^TGCAC
AseI 2102 ATTAAT AT^TAAT
AsnI 2102 ATTAAT AT^TAAT
AspI 1512 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3232 CCTAGG C^CTAGG
BcgI 305 GCATCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2175 TGATCA T^GATCA
BglII 833 AGATCT A^GATCT
BlnI 3232 CCTAGG C^CTAGG
BsaBI 4484 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 463 GAGACC GGTCTC(1/5)
BsaWI 4454 TCCGGT [AT]^CCGG[AT]
BsmI 3900 GAATGC GAATGC(1/-1)
BspLU11I 4625 ACATGT A^CATGT
BspMI 3549 GCAGGT ACCTGC(4/8)
Bst1107I 1726 GTATAC GTA^TAC
BstBI 793 TTCGAA TT^CGAA
BstEII 1484 GGTAACC G^GT[ACGT]ACC
BstPI 1484 GGTAACC G^GT[ACGT]ACC
BstZ17I 1726 GTATAC GTA^TAC
EaeI 2721 TGGCCG [CT]^GGCC[GA]
EcoO65I 1484 GGTAACC G^GT[ACGT]ACC
EcoT22I 2322 ATGCAT ATGCA^T
FbaI 2175 TGATCA T^GATCA
HincII 3915 GTTAAC GT[CT]^[GA]AC
HindII 3915 GTTAAC GT[CT]^[GA]AC
HpaI 3915 GTTAAC GTT^AAC
KspI 35 CCGCGG CCGC^GG
MamI 4484 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2150 CAATTG C^AATTG
MluI 1918 ACGCGT A^CGCGT
MunI 2150 CAATTG C^AATTG
NcoI 1814 CCATGG C^CATGG
NdeI 3683 CATATG CA^TATG
NheI 1637 GCTAGC G^CTAGC
NsiI 2322 ATGCAT ATGCA^T
NspV 793 TTCGAA TT^CGAA
PflFI 1512 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 2195 GTTTAAAC GTTT^AAAC
PshBI 2102 ATTAAT AT^TAAT
Psp1406I 1463 AACGTT AA^CGTT
SacII 35 CCGCGG CCGC^GG
ScaI 338 AGTACT AGT^ACT
SexAI 713 ACCTGGT A^CC[AT]GGT
SfiI 2722 GGCCGAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 793 TTCGAA TT^CGAA
SspI 2114 AATATT AAT^ATT
Tth111I 1512 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1066 GACGTC GACGT^C
1865 GACGTC
Acc65I 1809 GGTACC G^GTACC
3927 GGTACC
AccI 1726 GTATAC GT^[AC][GT]AC
4092 GTCTAC
AcyI 1066 GACGTC G[GA]^CG[CT]C
1865 GACGTC
AflII 586 CTTAAG C^TTAAG
3707 CTTAAG
AflIII 1918 ACGCGT A^C[GA][CT]GT
4625 ACATGT
AocI 1350 CCTGAGG CC^T[ACGT]AGG
2262 CCTCAGG
Asp700I 1447 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3579 GAACATTTTC
Asp718I 1809 GGTACC G^GTACC
3927 GGTACC
AvaI 190 CCCGAG C^[CT]CG[GA]G
214 CCCGAG
BamHI 269 GGATCC G^GATCC
630 GGATCC
BbvCI 1143 GCTGAGG CCTCAGC(-5/-2)
1761 GCTGAGG
BfrI 586 CTTAAG C^TTAAG
3707 CTTAAG
BglI 2723 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4276 GCCCATCTGGC
BlpI 1980 GCTGAGC GC^T[ACGT]AGC
2883 GCTTAGC
Bpu1102I 1980 GCTGAGC GC^T[ACGT]AGC
2883 GCTTAGC
BsaHI 1066 GACGTC G[GA]^CG[CT]C
1865 GACGTC
BsoBI 190 CCCGAG C^[CT]CG[GA]G
214 CCCGAG
Bsp1407I 740 TGTACA T^GTACA
1529 TGTACA
BspHI 1831 TCATGA T^CATGA
2178 TCATGA
BsrFI 178 GCCGGC [GA]^CCGG[CT]
550 GCCGGC
BsrGI 740 TGTACA T^GTACA
1529 TGTACA
Bsu36I 1350 CCTGAGG CC^T[ACGT]AGG
2262 CCTCAGG
CelII 1980 GCTGAGC GC^T[ACGT]AGC
2883 GCTTAGC
Cfr10I 178 GCCGGC [GA]^CCGG[CT]
550 GCCGGC
Eco81I 1350 CCTGAGG CC^T[ACGT]AGG
2262 CCTCAGG
EcoNI 1350 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4313 CCTTACCAAGG
Hin1I 1066 GACGTC G[GA]^CG[CT]C
1865 GACGTC
KpnI 1809 GGTACC GGTAC^C
3927 GGTACC
NaeI 178 GCCGGC GCC^GGC
550 GCCGGC
NgoMIV 178 GCCGGC G^CCGGC
550 GCCGGC
PpuMI 209 AGGTCCC [GA]G^G[AT]CC[CT]
317 GGGACCC
RcaI 1831 TCATGA T^CATGA
2178 TCATGA
SacI 1983 GAGCTC GAGCT^C
2959 GAGCTC
SphI 1365 GCATGC GCATG^C
1522 GCATGC
SspBI 740 TGTACA T^GTACA
1529 TGTACA
StuI 218 AGGCCT AGG^CCT
473 AGGCCT
XbaI 3779 TCTAGA T^CTAGA
4480 TCTAGA
XmnI 1447 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3579 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII