##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AccII AccIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
AspEI AspI AvaI AviII AvrII
BbeI BcgI BcnI BlnI BsaWI
BseAI BsiEI BsiWI BsoBI BspDI
BspEI BsrBI BsrFI BssHII BstAPI
BstBI BstUI CfoI Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I FseI FspI HaeII HapII
HhaI HinP1I HindIII HpaI HpaII
KasI KspI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
Tth111I XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 3466 GGTACC G^GTACC
AclI 1834 AACGTT AA^CGTT
AflII 1328 CTTAAG C^TTAAG
AocI 1156 CCTTAGG CC^T[ACGT]AGG
Asp718I 3466 GGTACC G^GTACC
AspHI 4545 GAGCAC G[AT]GC[AT]^C
AvaII 2125 GGTCC G^G[AT]CC
BamHI 2053 GGATCC G^GATCC
BclI 3136 TGATCA T^GATCA
BfrI 1328 CTTAAG C^TTAAG
BglI 1341 GCCCTTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3281 AGATCT A^GATCT
BsiHKAI 4545 GAGCAC G[AT]GC[AT]^C
Bsp1407I 4267 TGTACA T^GTACA
BsrGI 4267 TGTACA T^GTACA
BssSI 295 CTCGTG CACGAG(-5/-1)
Bst1107I 679 GTATAC GTA^TAC
BstXI 2318 CCAGGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 679 GTATAC GTA^TAC
Bsu36I 1156 CCTTAGG CC^T[ACGT]AGG
DraIII 2713 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1156 CCTTAGG CC^T[ACGT]AGG
EcoRI 3439 GAATTC G^AATTC
FauI 332 CCCGC CCCGC(4/6)
FbaI 3136 TGATCA T^GATCA
HincII 1606 GTTGAC GT[CT]^[GA]AC
HindII 1606 GTTGAC GT[CT]^[GA]AC
KpnI 3466 GGTACC GGTAC^C
PpuMI 2124 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 1834 AACGTT AA^CGTT
SapI 526 GAAGAGC GCTCTTC(1/4)
ScaI 2647 AGTACT AGT^ACT
SexAI 2121 ACCAGGT A^CC[AT]GGT
SmlI 1328 CTTAAG C^T[CT][GA]AG
SpeI 2097 ACTAGT A^CTAGT
SphI 4640 GCATGC GCATG^C
SspBI 4267 TGTACA T^GTACA
SspI 1541 AATATT AAT^ATT
StuI 2043 AGGCCT AGG^CCT
SwaI 2618 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 927 GACGTC GACGT^C
939 GACGTC
AccI 679 GTATAC GT^[AC][GT]AC
2874 GTAGAC
BalI 1348 TGGCCA TGG^CCA
3540 TGGCCA
BanII 1390 GAGCCC G[GA]GC[CT]^C
3238 GGGCTC
BbrPI 1207 CACGTG CAC^GTG
3904 CACGTG
BbvCI 458 CCTCAGC CCTCAGC(-5/-2)
757 CCTCAGC
BciVI 4798 GGATAC GTATCC(6/5)
1876 GTATCC
BlpI 1129 GCTAAGC GC^T[ACGT]AGC
3674 GCTTAGC
Bpu1102I 1129 GCTAAGC GC^T[ACGT]AGC
3674 GCTTAGC
BsaAI 1207 CACGTG [CT]AC^GT[GA]
3904 CACGTG
BsmI 4736 GAATGC GAATGC(1/-1)
1863 GCATTC
BspMI 1024 ACCTGC ACCTGC(4/8)
1841 ACCTGC
BstEII 2905 GGTCACC G^GT[ACGT]ACC
3184 GGTCACC
BstPI 2905 GGTCACC G^GT[ACGT]ACC
3184 GGTCACC
CelII 1129 GCTAAGC GC^T[ACGT]AGC
3674 GCTTAGC
DraII 2124 AGGTCCC [GA]G^G[ACGT]CC[CT]
2603 AGGCCCT
DrdI 1609 GACCTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1992 GACAGAGATGTC
EaeI 1348 TGGCCA [CT]^GGCC[GA]
3540 TGGCCA
EcoNI 3621 CCTCTTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4553 CCTTCTAAAGG
EcoO109I 2124 AGGTCCC [GA]G^G[ACGT]CC[CT]
2603 AGGCCCT
EcoO65I 2905 GGTCACC G^GT[ACGT]ACC
3184 GGTCACC
EcoRV 1646 GATATC GAT^ATC
4479 GATATC
EcoT22I 1072 ATGCAT ATGCA^T
4097 ATGCAT
HgaI 1146 GACGC GACGC(5/10)
1190 GACGC
MluNI 1348 TGGCCA TGG^CCA
3540 TGGCCA
MscI 1348 TGGCCA TGG^CCA
3540 TGGCCA
NcoI 503 CCATGG C^CATGG
2821 CCATGG
NsiI 1072 ATGCAT ATGCA^T
4097 ATGCAT
PmaCI 1207 CACGTG CAC^GTG
3904 CACGTG
PmlI 1207 CACGTG CAC^GTG
3904 CACGTG
TaqI 2574 TCGA T^CGA
3947 TCGA
TthHB8I 2574 TCGA T^CGA
3947 TCGA
XbaI 471 TCTAGA T^CTAGA
4362 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI BanI BbsI BbvI BfaI
BmyI BpmI BpuAI BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspHI BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
Hin1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PshBI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII