################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AccII AccIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I AspEI AspI AvaI AviII AvrII BbeI BcgI BcnI BlnI BsaWI BseAI BsiEI BsiWI BsoBI BspDI BspEI BsrBI BsrFI BssHII BstAPI BstBI BstUI CfoI Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI HaeII HapII HhaI HinP1I HindIII HpaI HpaII KasI KspI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I Tth111I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 3466 GGTACC G^GTACC AclI 1834 AACGTT AA^CGTT AflII 1328 CTTAAG C^TTAAG AocI 1156 CCTTAGG CC^T[ACGT]AGG Asp718I 3466 GGTACC G^GTACC AspHI 4545 GAGCAC G[AT]GC[AT]^C AvaII 2125 GGTCC G^G[AT]CC BamHI 2053 GGATCC G^GATCC BclI 3136 TGATCA T^GATCA BfrI 1328 CTTAAG C^TTAAG BglI 1341 GCCCTTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3281 AGATCT A^GATCT BsiHKAI 4545 GAGCAC G[AT]GC[AT]^C Bsp1407I 4267 TGTACA T^GTACA BsrGI 4267 TGTACA T^GTACA BssSI 295 CTCGTG CACGAG(-5/-1) Bst1107I 679 GTATAC GTA^TAC BstXI 2318 CCAGGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 679 GTATAC GTA^TAC Bsu36I 1156 CCTTAGG CC^T[ACGT]AGG DraIII 2713 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1156 CCTTAGG CC^T[ACGT]AGG EcoRI 3439 GAATTC G^AATTC FauI 332 CCCGC CCCGC(4/6) FbaI 3136 TGATCA T^GATCA HincII 1606 GTTGAC GT[CT]^[GA]AC HindII 1606 GTTGAC GT[CT]^[GA]AC KpnI 3466 GGTACC GGTAC^C PpuMI 2124 AGGTCCC [GA]G^G[AT]CC[CT] Psp1406I 1834 AACGTT AA^CGTT SapI 526 GAAGAGC GCTCTTC(1/4) ScaI 2647 AGTACT AGT^ACT SexAI 2121 ACCAGGT A^CC[AT]GGT SmlI 1328 CTTAAG C^T[CT][GA]AG SpeI 2097 ACTAGT A^CTAGT SphI 4640 GCATGC GCATG^C SspBI 4267 TGTACA T^GTACA SspI 1541 AATATT AAT^ATT StuI 2043 AGGCCT AGG^CCT SwaI 2618 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AatII 927 GACGTC GACGT^C 939 GACGTC AccI 679 GTATAC GT^[AC][GT]AC 2874 GTAGAC BalI 1348 TGGCCA TGG^CCA 3540 TGGCCA BanII 1390 GAGCCC G[GA]GC[CT]^C 3238 GGGCTC BbrPI 1207 CACGTG CAC^GTG 3904 CACGTG BbvCI 458 CCTCAGC CCTCAGC(-5/-2) 757 CCTCAGC BciVI 4798 GGATAC GTATCC(6/5) 1876 GTATCC BlpI 1129 GCTAAGC GC^T[ACGT]AGC 3674 GCTTAGC Bpu1102I 1129 GCTAAGC GC^T[ACGT]AGC 3674 GCTTAGC BsaAI 1207 CACGTG [CT]AC^GT[GA] 3904 CACGTG BsmI 4736 GAATGC GAATGC(1/-1) 1863 GCATTC BspMI 1024 ACCTGC ACCTGC(4/8) 1841 ACCTGC BstEII 2905 GGTCACC G^GT[ACGT]ACC 3184 GGTCACC BstPI 2905 GGTCACC G^GT[ACGT]ACC 3184 GGTCACC CelII 1129 GCTAAGC GC^T[ACGT]AGC 3674 GCTTAGC DraII 2124 AGGTCCC [GA]G^G[ACGT]CC[CT] 2603 AGGCCCT DrdI 1609 GACCTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1992 GACAGAGATGTC EaeI 1348 TGGCCA [CT]^GGCC[GA] 3540 TGGCCA EcoNI 3621 CCTCTTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4553 CCTTCTAAAGG EcoO109I 2124 AGGTCCC [GA]G^G[ACGT]CC[CT] 2603 AGGCCCT EcoO65I 2905 GGTCACC G^GT[ACGT]ACC 3184 GGTCACC EcoRV 1646 GATATC GAT^ATC 4479 GATATC EcoT22I 1072 ATGCAT ATGCA^T 4097 ATGCAT HgaI 1146 GACGC GACGC(5/10) 1190 GACGC MluNI 1348 TGGCCA TGG^CCA 3540 TGGCCA MscI 1348 TGGCCA TGG^CCA 3540 TGGCCA NcoI 503 CCATGG C^CATGG 2821 CCATGG NsiI 1072 ATGCAT ATGCA^T 4097 ATGCAT PmaCI 1207 CACGTG CAC^GTG 3904 CACGTG PmlI 1207 CACGTG CAC^GTG 3904 CACGTG TaqI 2574 TCGA T^CGA 3947 TCGA TthHB8I 2574 TCGA T^CGA 3947 TCGA XbaI 471 TCTAGA T^CTAGA 4362 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI BanI BbsI BbvI BfaI BmyI BpmI BpuAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspHI BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DsaI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII Hin1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PshBI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII