##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI AgeI AhdI ApaI AscI
AseI AsnI Asp700I AspEI BamHI
BbvCI BclI BsiWI BspDI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoT22I FbaI
FseI HindIII HpaI KspI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SspI SwaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 3296 GGTACC G^GTACC
AccI 2939 GTCGAC GT^[AC][GT]AC
AccIII 4660 TCCGGA T^CCGGA
AflII 1529 CTTAAG C^TTAAG
Alw44I 3404 GTGCAC G^TGCAC
ApaLI 3404 GTGCAC G^TGCAC
Asp718I 3296 GGTACC G^GTACC
AspI 2942 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4675 CCTAGG C^CTAGG
BbrPI 880 CACGTG CAC^GTG
BcnI 2002 CCGGG CC^[GC]GG
BfrI 1529 CTTAAG C^TTAAG
BlnI 4675 CCTAGG C^CTAGG
BsaBI 1219 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4660 TCCGGA [AT]^CCGG[AT]
BseAI 4660 TCCGGA T^CCGGA
BsiEI 1999 CGACCG CG[GA][CT]^CG
BsmBI 107 GAGACG CGTCTC(1/5)
Bsp1407I 1962 TGTACA T^GTACA
BspEI 4660 TCCGGA T^CCGGA
BspHI 168 TCATGA T^CATGA
BspLU11I 4814 ACATGT A^CATGT
BsrGI 1962 TGTACA T^GTACA
DraI 2765 TTTAAA TTT^AAA
DraIII 2857 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 518 GATATC GAT^ATC
HincII 2939 GTCGAC GT[CT]^[GA]AC
HindII 2939 GTCGAC GT[CT]^[GA]AC
KpnI 3296 GGTACC GGTAC^C
MamI 1219 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4660 TCCGGA T^CCGGA
NciI 2002 CCGGG CC^[GC]GG
PaeR7I 942 CTCGAG C^TCGAG
PflFI 2942 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 880 CACGTG CAC^GTG
PmlI 880 CACGTG CAC^GTG
PshAI 3966 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 168 TCATGA T^CATGA
SalI 2939 GTCGAC G^TCGAC
SexAI 2943 ACCTGGT A^CC[AT]GGT
SphI 2322 GCATGC GCATG^C
SspBI 1962 TGTACA T^GTACA
Tth111I 2942 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4393 TCTAGA T^CTAGA
XhoI 942 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 2422 AAATTT [GA]^AATT[CT]
2793 AAATTT
AocI 926 CCTGAGG CC^T[ACGT]AGG
3365 CCTCAGG
ApoI 2422 AAATTT [GA]^AATT[CT]
2793 AAATTT
AviII 3154 TGCGCA TGC^GCA
3588 TGCGCA
BbeI 2883 GGCGCC GGCGC^C
3629 GGCGCC
BcgI 4608 CGAGGGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2010 GCAGGCTCCTCG
BfaI 4394 CTAG C^TAG
4676 CTAG
BglII 2070 AGATCT A^GATCT
4102 AGATCT
BlpI 3334 GCTCAGC GC^T[ACGT]AGC
3640 GCTGAGC
Bpu1102I 3334 GCTCAGC GC^T[ACGT]AGC
3640 GCTGAGC
BsmI 4367 GAATGC GAATGC(1/-1)
2353 GCATTC
BsrFI 2176 GCCGGC [GA]^CCGG[CT]
3626 GCCGGC
BstXI 222 CCACACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1614 CCAACCTCCTGG
Bsu36I 926 CCTGAGG CC^T[ACGT]AGG
3365 CCTCAGG
CelII 3334 GCTCAGC GC^T[ACGT]AGC
3640 GCTGAGC
Cfr10I 2176 GCCGGC [GA]^CCGG[CT]
3626 GCCGGC
DrdI 1805 GACGTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2429 GACGATCTGGTC
Eco81I 926 CCTGAGG CC^T[ACGT]AGG
3365 CCTCAGG
FspI 3154 TGCGCA TGC^GCA
3588 TGCGCA
KasI 2883 GGCGCC G^GCGCC
3629 GGCGCC
MaeI 4394 CTAG C^TAG
4676 CTAG
NaeI 2176 GCCGGC GCC^GGC
3626 GCCGGC
NarI 2883 GGCGCC GG^CGCC
3629 GGCGCC
NgoMIV 2176 GCCGGC G^CCGGC
3626 GCCGGC
PflMI 2363 CCATACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2856 CCACCGTGTGG
SapI 2645 GAAGAGC GCTCTTC(1/4)
112 GCTCTTC
SbfI 3109 CCTGCAGG CCTGCA^GG
3259 CCTGCAGG
SfoI 2883 GGCGCC GGC^GCC
3629 GGCGCC
Sse8387I 3109 CCTGCAGG CCTGCA^GG
3259 CCTGCAGG
Van91I 2363 CCATACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2856 CCACCGTGTGG
XcmI 1299 CCAGGTTAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1651 CCAGAGCTTTTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI Aor51HI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BciVI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII