################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AatII AccIII AclI AgeI AscI AseI AsnI AviII BcgI BsaBI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrFI BssHII Bst1107I BstBI BstZ17I Cfr10I ClaI EagI EclXI Eco52I FseI FspI HpaI KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI SacII SalI SfiI SfuI SgrAI SnaBI SspI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 2643 GGTACC G^GTACC AflII 3334 CTTAAG C^TTAAG AflIII 1395 ACACGT A^C[GA][CT]GT Alw44I 618 GTGCAC G^TGCAC Aor51HI 670 AGCGCT AGC^GCT ApaLI 618 GTGCAC G^TGCAC Asp700I 5039 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2643 GGTACC G^GTACC AspI 4261 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3827 CCCGGG C^[CT]CG[GA]G AvrII 101 CCTAGG C^CTAGG BalI 2096 TGGCCA TGG^CCA BbrPI 1396 CACGTG CAC^GTG BfrI 3334 CTTAAG C^TTAAG BglI 4306 GCCACTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 101 CCTAGG C^CTAGG BsaAI 1396 CACGTG [CT]AC^GT[GA] BsmI 336 GCATTC GAATGC(1/-1) BsoBI 3827 CCCGGG C^[CT]CG[GA]G Bsp1407I 1594 TGTACA T^GTACA BsrDI 3637 CATTGC GCAATG(2/0) BsrGI 1594 TGTACA T^GTACA CpoI 1873 CGGACCG CG^G[AT]CCG DraI 5151 TTTAAA TTT^AAA DraIII 472 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 670 AGCGCT AGC^GCT EcoRV 2814 GATATC GAT^ATC EcoT22I 5134 ATGCAT ATGCA^T HincII 1529 GTCAAC GT[CT]^[GA]AC HindII 1529 GTCAAC GT[CT]^[GA]AC KpnI 2643 GGTACC GGTAC^C MluNI 2096 TGGCCA TGG^CCA MscI 2096 TGGCCA TGG^CCA NheI 2726 GCTAGC G^CTAGC NsiI 5134 ATGCAT ATGCA^T PflFI 4261 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3223 CCAGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1396 CACGTG CAC^GTG PmlI 1396 CACGTG CAC^GTG PshAI 2929 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 1873 CGGACCG CG^G[AT]CCG SbfI 2486 CCTGCAGG CCTGCA^GG ScaI 5167 AGTACT AGT^ACT SexAI 19 ACCAGGT A^CC[AT]GGT SmaI 3827 CCCGGG CCC^GGG Sse8387I 2486 CCTGCAGG CCTGCA^GG SspBI 1594 TGTACA T^GTACA SwaI 5150 ATTTAAAT ATTT^AAAT Tth111I 4261 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3223 CCAGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 3827 CCCGGG C^CCGGG XmaI 3827 CCCGGG C^CCGGG XmnI 5039 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 1824 GTAGAC GT^[AC][GT]AC 3950 GTAGAC AhdI 2581 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3008 GACATGATGTC AocI 731 CCTGAGG CC^T[ACGT]AGG 4649 CCTAAGG AspEI 2581 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3008 GACATGATGTC BlpI 1676 GCTGAGC GC^T[ACGT]AGC 4726 GCTCAGC Bpu1102I 1676 GCTGAGC GC^T[ACGT]AGC 4726 GCTCAGC BsaWI 3954 ACCGGA [AT]^CCGG[AT] 4027 ACCGGA BsmBI 2588 CGTCTC CGTCTC(1/5) 5158 CGTCTC BspHI 2011 TCATGA T^CATGA 2659 TCATGA BsrBI 2167 GAGCGG CCGCTC(-3/-3) 2688 GAGCGG BssSI 4561 CTCGTG CACGAG(-5/-1) 5100 CTCGTG BstEII 105 GGTGACC G^GT[ACGT]ACC 4546 GGTGACC BstPI 105 GGTGACC G^GT[ACGT]ACC 4546 GGTGACC Bsu36I 731 CCTGAGG CC^T[ACGT]AGG 4649 CCTAAGG CelII 1676 GCTGAGC GC^T[ACGT]AGC 4726 GCTCAGC DrdI 3154 GACTCATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3535 GACAGGAAGGTC Eam1105I 2581 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3008 GACATGATGTC Eco81I 731 CCTGAGG CC^T[ACGT]AGG 4649 CCTAAGG EcoO65I 105 GGTGACC G^GT[ACGT]ACC 4546 GGTGACC EcoRI 1323 GAATTC G^AATTC 4914 GAATTC RcaI 2011 TCATGA T^CATGA 2659 TCATGA SapI 2535 GAAGAGC GCTCTTC(1/4) 47 GCTCTTC SpeI 614 ACTAGT A^CTAGT 1083 ACTAGT SphI 466 GCATGC GCATG^C 2327 GCATGC StuI 524 AGGCCT AGG^CCT 4239 AGGCCT XbaI 80 TCTAGA T^CTAGA 5162 TCTAGA XcmI 1108 CCAACAGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2938 CCACAACCCTGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaII BamHI BanI BanII BbeI BbsI BbvCI BbvI BciVI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII