##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccIII AclI AgeI AscI
AseI AsnI AviII BcgI BsaBI
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BsrFI BssHII Bst1107I BstBI
BstZ17I Cfr10I ClaI EagI EclXI
Eco52I FseI FspI HpaI KspI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI SacII SalI
SfiI SfuI SgrAI SnaBI SspI
XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 2643 GGTACC G^GTACC
AflII 3334 CTTAAG C^TTAAG
AflIII 1395 ACACGT A^C[GA][CT]GT
Alw44I 618 GTGCAC G^TGCAC
Aor51HI 670 AGCGCT AGC^GCT
ApaLI 618 GTGCAC G^TGCAC
Asp700I 5039 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2643 GGTACC G^GTACC
AspI 4261 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3827 CCCGGG C^[CT]CG[GA]G
AvrII 101 CCTAGG C^CTAGG
BalI 2096 TGGCCA TGG^CCA
BbrPI 1396 CACGTG CAC^GTG
BfrI 3334 CTTAAG C^TTAAG
BglI 4306 GCCACTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 101 CCTAGG C^CTAGG
BsaAI 1396 CACGTG [CT]AC^GT[GA]
BsmI 336 GCATTC GAATGC(1/-1)
BsoBI 3827 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1594 TGTACA T^GTACA
BsrDI 3637 CATTGC GCAATG(2/0)
BsrGI 1594 TGTACA T^GTACA
CpoI 1873 CGGACCG CG^G[AT]CCG
DraI 5151 TTTAAA TTT^AAA
DraIII 472 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 670 AGCGCT AGC^GCT
EcoRV 2814 GATATC GAT^ATC
EcoT22I 5134 ATGCAT ATGCA^T
HincII 1529 GTCAAC GT[CT]^[GA]AC
HindII 1529 GTCAAC GT[CT]^[GA]AC
KpnI 2643 GGTACC GGTAC^C
MluNI 2096 TGGCCA TGG^CCA
MscI 2096 TGGCCA TGG^CCA
NheI 2726 GCTAGC G^CTAGC
NsiI 5134 ATGCAT ATGCA^T
PflFI 4261 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3223 CCAGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1396 CACGTG CAC^GTG
PmlI 1396 CACGTG CAC^GTG
PshAI 2929 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 1873 CGGACCG CG^G[AT]CCG
SbfI 2486 CCTGCAGG CCTGCA^GG
ScaI 5167 AGTACT AGT^ACT
SexAI 19 ACCAGGT A^CC[AT]GGT
SmaI 3827 CCCGGG CCC^GGG
Sse8387I 2486 CCTGCAGG CCTGCA^GG
SspBI 1594 TGTACA T^GTACA
SwaI 5150 ATTTAAAT ATTT^AAAT
Tth111I 4261 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3223 CCAGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3827 CCCGGG C^CCGGG
XmaI 3827 CCCGGG C^CCGGG
XmnI 5039 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1824 GTAGAC GT^[AC][GT]AC
3950 GTAGAC
AhdI 2581 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3008 GACATGATGTC
AocI 731 CCTGAGG CC^T[ACGT]AGG
4649 CCTAAGG
AspEI 2581 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3008 GACATGATGTC
BlpI 1676 GCTGAGC GC^T[ACGT]AGC
4726 GCTCAGC
Bpu1102I 1676 GCTGAGC GC^T[ACGT]AGC
4726 GCTCAGC
BsaWI 3954 ACCGGA [AT]^CCGG[AT]
4027 ACCGGA
BsmBI 2588 CGTCTC CGTCTC(1/5)
5158 CGTCTC
BspHI 2011 TCATGA T^CATGA
2659 TCATGA
BsrBI 2167 GAGCGG CCGCTC(-3/-3)
2688 GAGCGG
BssSI 4561 CTCGTG CACGAG(-5/-1)
5100 CTCGTG
BstEII 105 GGTGACC G^GT[ACGT]ACC
4546 GGTGACC
BstPI 105 GGTGACC G^GT[ACGT]ACC
4546 GGTGACC
Bsu36I 731 CCTGAGG CC^T[ACGT]AGG
4649 CCTAAGG
CelII 1676 GCTGAGC GC^T[ACGT]AGC
4726 GCTCAGC
DrdI 3154 GACTCATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3535 GACAGGAAGGTC
Eam1105I 2581 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3008 GACATGATGTC
Eco81I 731 CCTGAGG CC^T[ACGT]AGG
4649 CCTAAGG
EcoO65I 105 GGTGACC G^GT[ACGT]ACC
4546 GGTGACC
EcoRI 1323 GAATTC G^AATTC
4914 GAATTC
RcaI 2011 TCATGA T^CATGA
2659 TCATGA
SapI 2535 GAAGAGC GCTCTTC(1/4)
47 GCTCTTC
SpeI 614 ACTAGT A^CTAGT
1083 ACTAGT
SphI 466 GCATGC GCATG^C
2327 GCATGC
StuI 524 AGGCCT AGG^CCT
4239 AGGCCT
XbaI 80 TCTAGA T^CTAGA
5162 TCTAGA
XcmI 1108 CCAACAGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2938 CCACAACCCTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaII BamHI BanI BanII
BbeI BbsI BbvCI BbvI BciVI
BclI BcnI BfaI BglII BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII