##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Aor51HI AscI AseI AsnI
Asp718I AspI AviII BbeI BglII
BsaHI BseAI BsiEI BsmBI BspDI
BspEI BspHI BssHII BstAPI ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I FseI FspI Hin1I HincII
HindII HpaI KasI KpnI KspI
MluI MroI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SmaI Sse8387I SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 1659 CGCG CG^CG
AflII 3229 CTTAAG C^TTAAG
AhdI 2031 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3863 GTGCAC G^TGCAC
ApaLI 3863 GTGCAC G^TGCAC
AspEI 2031 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 2580 GGCACC G^G[CT][GA]CC
BbvCI 720 GCTGAGG CCTCAGC(-5/-2)
BcgI 487 GCATTCTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2245 GTATCC GTATCC(6/5)
BclI 4355 TGATCA T^GATCA
BfrI 3229 CTTAAG C^TTAAG
BglI 3306 GCCTTACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4409 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4409 GCTGAGC GC^T[ACGT]AGC
BsiWI 1031 CGTACG C^GTACG
BspLU11I 3133 ACATGT A^CATGT
BsrBI 5140 CCGCTC CCGCTC(-3/-3)
BsrDI 4106 GCAATG GCAATG(2/0)
BsrFI 1169 GCCGGT [GA]^CCGG[CT]
Bst1107I 3109 GTATAC GTA^TAC
BstBI 495 TTCGAA TT^CGAA
BstEII 1172 GGTTACC G^GT[ACGT]ACC
BstPI 1172 GGTTACC G^GT[ACGT]ACC
BstUI 1659 CGCG CG^CG
BstZ17I 3109 GTATAC GTA^TAC
CelII 4409 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1169 GCCGGT [GA]^CCGG[CT]
Eam1105I 2031 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1172 GGTTACC G^GT[ACGT]ACC
EcoRV 974 GATATC GAT^ATC
FbaI 4355 TGATCA T^GATCA
HaeII 39 GGCGCT [GA]GCGC^[CT]
HgaI 4644 GCGTC GACGC(5/10)
MvnI 1659 CGCG CG^CG
NcoI 4247 CCATGG C^CATGG
NspV 495 TTCGAA TT^CGAA
PaeR7I 5143 CTCGAG C^TCGAG
SapI 2829 GCTCTTC GCTCTTC(1/4)
ScaI 92 AGTACT AGT^ACT
SfuI 495 TTCGAA TT^CGAA
SpeI 3237 ACTAGT A^CTAGT
XcmI 189 CCATGTGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 5143 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AocI 3391 CCTAAGG CC^T[ACGT]AGG
3919 CCTCAGG
ApaI 3870 GGGCCC GGGCC^C
4461 GGGCCC
AvaI 1883 CTCGGG C^[CT]CG[GA]G
5143 CTCGAG
AvrII 536 CCTAGG C^CTAGG
4583 CCTAGG
BalI 322 TGGCCA TGG^CCA
4373 TGGCCA
BamHI 150 GGATCC G^GATCC
4595 GGATCC
BbrPI 234 CACGTG CAC^GTG
3213 CACGTG
BlnI 536 CCTAGG C^CTAGG
4583 CCTAGG
BsaBI 507 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4391 GATTTTTATC
BsaWI 1684 ACCGGA [AT]^CCGG[AT]
2163 ACCGGA
BsoBI 1883 CTCGGG C^[CT]CG[GA]G
5143 CTCGAG
Bsp1407I 2747 TGTACA T^GTACA
4748 TGTACA
BsrGI 2747 TGTACA T^GTACA
4748 TGTACA
BssSI 283 CACGAG CACGAG(-5/-1)
4804 CACGAG
Bsu36I 3391 CCTAAGG CC^T[ACGT]AGG
3919 CCTCAGG
DrdI 3947 GACTTTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4022 GACTGTGAGGTC
DsaI 2041 CCGTGG C^C[GA][CT]GG
4247 CCATGG
Eco81I 3391 CCTAAGG CC^T[ACGT]AGG
3919 CCTCAGG
EcoRI 215 GAATTC G^AATTC
3425 GAATTC
EcoT22I 3666 ATGCAT ATGCA^T
3826 ATGCAT
FauI 3974 CCCGC CCCGC(4/6)
2378 GCGGG
MamI 507 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4391 GATTTTTATC
MfeI 2680 CAATTG C^AATTG
5177 CAATTG
MluNI 322 TGGCCA TGG^CCA
4373 TGGCCA
MscI 322 TGGCCA TGG^CCA
4373 TGGCCA
MunI 2680 CAATTG C^AATTG
5177 CAATTG
NsiI 3666 ATGCAT ATGCA^T
3826 ATGCAT
PmaCI 234 CACGTG CAC^GTG
3213 CACGTG
PmlI 234 CACGTG CAC^GTG
3213 CACGTG
SacI 56 GAGCTC GAGCT^C
264 GAGCTC
SspBI 2747 TGTACA T^GTACA
4748 TGTACA
SspI 1840 AATATT AAT^ATT
2053 AATATT
StuI 317 AGGCCT AGG^CCT
3368 AGGCCT
XbaI 867 TCTAGA T^CTAGA
5027 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SnaBI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI