##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccI AccIII AclI AflIII
AgeI Aor51HI AscI AspI AviII
BbeI BcgI BciVI BclI BglI
BglII BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BssHII Bst1107I
BstBI BstZ17I ClaI CpoI EagI
EclXI Eco47III Eco52I FbaI FspI
HpaI KasI KspI MluI MroI
NarI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SfiI
SfoI SfuI SnaBI SpeI SphI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 4218 CGCG CG^CG
AcyI 4966 GGCGTC G[GA]^CG[CT]C
AhdI 2517 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2517 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4349 CCTAGG C^CTAGG
BalI 2314 TGGCCA TGG^CCA
BbrPI 5452 CACGTG CAC^GTG
BlnI 4349 CCTAGG C^CTAGG
BsaAI 5452 CACGTG [CT]AC^GT[GA]
BsaHI 4966 GGCGTC G[GA]^CG[CT]C
BsaWI 292 ACCGGA [AT]^CCGG[AT]
Bsp1407I 5263 TGTACA T^GTACA
BsrBI 3564 GAGCGG CCGCTC(-3/-3)
BsrGI 5263 TGTACA T^GTACA
BssSI 562 CTCGTG CACGAG(-5/-1)
BstUI 4218 CGCG CG^CG
BstXI 4418 CCAGGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 972 TTTAAA TTT^AAA
DraIII 5678 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4308 GACTTGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2314 TGGCCA [CT]^GGCC[GA]
Eam1105I 2517 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 132 GGCCGGCC GGCCGG^CC
HaeII 4249 AGCGCC [GA]GCGC^[CT]
HgaI 4967 GCGTC GACGC(5/10)
Hin1I 4966 GGCGTC G[GA]^CG[CT]C
MaeII 5453 ACGT A^CGT
MfeI 767 CAATTG C^AATTG
MluNI 2314 TGGCCA TGG^CCA
MscI 2314 TGGCCA TGG^CCA
MunI 767 CAATTG C^AATTG
MvnI 4218 CGCG CG^CG
NheI 1563 GCTAGC G^CTAGC
PmaCI 5452 CACGTG CAC^GTG
PmlI 5452 CACGTG CAC^GTG
PshAI 3171 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 4443 CCTGCAGG CCTGCA^GG
SgrAI 2 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 138 CCCGGG CCC^GGG
Sse8387I 4443 CCTGCAGG CCTGCA^GG
SspBI 5263 TGTACA T^GTACA
XmaCI 138 CCCGGG C^CCGGG
XmaI 138 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2835 GGTACC G^GTACC
4389 GGTACC
AflII 3377 CTTAAG C^TTAAG
3468 CTTAAG
ApaI 920 GGGCCC GGGCC^C
2156 GGGCCC
AseI 3277 ATTAAT AT^TAAT
4115 ATTAAT
AsnI 3277 ATTAAT AT^TAAT
4115 ATTAAT
Asp700I 1540 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3573 GAAGTTCTTC
Asp718I 2835 GGTACC G^GTACC
4389 GGTACC
BbsI 3085 GAAGAC GAAGAC(2/6)
1652 GTCTTC
BfrI 3377 CTTAAG C^TTAAG
3468 CTTAAG
BlpI 1399 GCTGAGC GC^T[ACGT]AGC
3608 GCTCAGC
Bpu1102I 1399 GCTGAGC GC^T[ACGT]AGC
3608 GCTCAGC
BpuAI 3085 GAAGAC GAAGAC(2/6)
1652 GTCTTC
BsaBI 3230 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4036 GATTGGGATC
BsaI 1791 GAGACC GGTCTC(1/5)
3732 GGTCTC
BspHI 2827 TCATGA T^CATGA
3506 TCATGA
CelII 1399 GCTGAGC GC^T[ACGT]AGC
3608 GCTCAGC
EcoRI 1544 GAATTC G^AATTC
5439 GAATTC
EcoRV 1497 GATATC GAT^ATC
1985 GATATC
EcoT22I 1447 ATGCAT ATGCA^T
4768 ATGCAT
HindIII 4642 AAGCTT A^AGCTT
5251 AAGCTT
KpnI 2835 GGTACC GGTAC^C
4389 GGTACC
MamI 3230 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4036 GATTGGGATC
NdeI 3173 CATATG CA^TATG
5420 CATATG
NsiI 1447 ATGCAT ATGCA^T
4768 ATGCAT
PshBI 3277 ATTAAT AT^TAAT
4115 ATTAAT
RcaI 2827 TCATGA T^CATGA
3506 TCATGA
SexAI 421 ACCTGGT A^CC[AT]GGT
898 ACCTGGT
SspI 2274 AATATT AAT^ATT
3443 AATATT
StuI 958 AGGCCT AGG^CCT
4328 AGGCCT
XbaI 2046 TCTAGA T^CTAGA
4232 TCTAGA
XmnI 1540 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3573 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI AocI ApaLI ApoI
AspHI AvaI AvaII BamHI BanI
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII