##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp718I AspEI AvrII
BamHI BciVI BcnI BglI BlnI
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BssHII BssSI BstUI BstXI
Bsu36I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV FseI HgaI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NciI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SgrAI SmaI
SnaBI SphI Sse8387I SwaI XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcyI 3446 GGCGCC G[GA]^CG[CT]C
Alw44I 2009 GTGCAC G^TGCAC
ApaI 3536 GGGCCC GGGCC^C
ApaLI 2009 GTGCAC G^TGCAC
AspI 4777 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 252 CCCGAG C^[CT]CG[GA]G
AvaII 3889 GGTCC G^G[AT]CC
AviII 2169 TGCGCA TGC^GCA
BbeI 3446 GGCGCC GGCGC^C
BbrPI 1691 CACGTG CAC^GTG
BbvCI 79 GCTGAGG CCTCAGC(-5/-2)
BcgI 4058 GCAAGCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 210 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 210 GCTAAGC GC^T[ACGT]AGC
BsaAI 1691 CACGTG [CT]AC^GT[GA]
BsaBI 2195 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3446 GGCGCC G[GA]^CG[CT]C
BsaWI 2042 TCCGGT [AT]^CCGG[AT]
BsmBI 4507 GAGACG CGTCTC(1/5)
BsoBI 252 CCCGAG C^[CT]CG[GA]G
BspHI 2191 TCATGA T^CATGA
BsrBI 3532 GAGCGG CCGCTC(-3/-3)
Bst1107I 399 GTATAC GTA^TAC
BstAPI 257 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2965 TTCGAA TT^CGAA
BstZ17I 399 GTATAC GTA^TAC
CelII 210 GCTAAGC GC^T[ACGT]AGC
EcoNI 1747 CCTCCACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3610 GAATTC G^AATTC
EcoT22I 4243 ATGCAT ATGCA^T
FspI 2169 TGCGCA TGC^GCA
Hin1I 3446 GGCGCC G[GA]^CG[CT]C
HpaI 2285 GTTAAC GTT^AAC
KasI 3446 GGCGCC G^GCGCC
MamI 2195 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3446 GGCGCC GG^CGCC
NcoI 3858 CCATGG C^CATGG
NsiI 4243 ATGCAT ATGCA^T
NspV 2965 TTCGAA TT^CGAA
PflFI 4777 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1674 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1691 CACGTG CAC^GTG
PmlI 1691 CACGTG CAC^GTG
RcaI 2191 TCATGA T^CATGA
ScaI 2858 AGTACT AGT^ACT
SfoI 3446 GGCGCC GGC^GCC
SfuI 2965 TTCGAA TT^CGAA
SpeI 2511 ACTAGT A^CTAGT
SspI 718 AATATT AAT^ATT
Tth111I 4777 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1674 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 399 GTATAC GT^[AC][GT]AC
4735 GTCTAC
AflII 536 CTTAAG C^TTAAG
1153 CTTAAG
Asp700I 2586 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3173 GAAGTTATTC
BclI 2597 TGATCA T^GATCA
3317 TGATCA
BfrI 536 CTTAAG C^TTAAG
1153 CTTAAG
BglII 2560 AGATCT A^GATCT
4718 AGATCT
BseRI 1396 CTCCTC GAGGAG(10/8)
4018 GAGGAG
Bsp1407I 1874 TGTACA T^GTACA
3088 TGTACA
BspMI 3401 ACCTGC ACCTGC(4/8)
2691 GCAGGT
BsrFI 3069 GCCGGT [GA]^CCGG[CT]
3242 ACCGGC
BsrGI 1874 TGTACA T^GTACA
3088 TGTACA
BstEII 2976 GGTCACC G^GT[ACGT]ACC
3356 GGTTACC
BstPI 2976 GGTCACC G^GT[ACGT]ACC
3356 GGTTACC
Cfr10I 3069 GCCGGT [GA]^CCGG[CT]
3242 ACCGGC
DraIII 3716 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4562 CACTTTGTG
EcoO65I 2976 GGTCACC G^GT[ACGT]ACC
3356 GGTTACC
EcoT14I 604 CCTTGG C^C[AT][AT]GG
3858 CCATGG
FauI 4407 CCCGC CCCGC(4/6)
3534 GCGGG
FbaI 2597 TGATCA T^GATCA
3317 TGATCA
HaeII 1975 AGCGCC [GA]GCGC^[CT]
3446 GGCGCC
HincII 2285 GTTAAC GT[CT]^[GA]AC
3988 GTCAAC
HindII 2285 GTTAAC GT[CT]^[GA]AC
3988 GTCAAC
HindIII 478 AAGCTT A^AGCTT
834 AAGCTT
MspA1I 3200 CAGCTG C[AC]G^C[GT]G
3313 CAGCTG
NspI 1792 GCATGT [GA]CATG^[CT]
2553 GCATGT
PvuII 3200 CAGCTG CAG^CTG
3313 CAGCTG
SapI 1971 GAAGAGC GCTCTTC(1/4)
4535 GCTCTTC
SspBI 1874 TGTACA T^GTACA
3088 TGTACA
StuI 2260 AGGCCT AGG^CCT
3281 AGGCCT
StyI 604 CCTTGG C^C[AT][AT]GG
3858 CCATGG
TaqI 2400 TCGA T^CGA
2966 TCGA
TthHB8I 2400 TCGA T^CGA
2966 TCGA
XmnI 2586 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3173 GAAGTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI BalI
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII