################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII AccIII AclI AflII AscI AseI AsnI Asp700I AspI BbeI BbrPI BcgI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BseAI BsiWI BsmI BspEI BspLU11I BssHII Bst1107I BstZ17I CelII CpoI DraIII EcoRI EcoRV FseI KasI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NruI PacI PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SbfI SfoI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 37 AAATTT [GA]^AATT[CT] AgeI 1131 ACCGGT A^CCGGT AhdI 5223 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4777 GTGCAC G^TGCAC Aor51HI 2505 AGCGCT AGC^GCT ApaLI 4777 GTGCAC G^TGCAC ApoI 37 AAATTT [GA]^AATT[CT] AspEI 5223 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 5265 GGATCC G^GATCC BclI 4978 TGATCA T^GATCA BsaI 1386 GGTCTC GGTCTC(1/5) BsmBI 82 GAGACG CGTCTC(1/5) Bsp1407I 4110 TGTACA T^GTACA BspDI 1309 ATCGAT AT^CGAT BsrBI 1611 GAGCGG CCGCTC(-3/-3) BsrGI 4110 TGTACA T^GTACA BstBI 21 TTCGAA TT^CGAA ClaI 1309 ATCGAT AT^CGAT DraI 2851 TTTAAA TTT^AAA DrdI 1695 GACAGGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 5223 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2505 AGCGCT AGC^GCT FbaI 4978 TGATCA T^GATCA HindIII 1997 AAGCTT A^AGCTT NdeI 1831 CATATG CA^TATG NotI 3322 GCGGCCGC GC^GGCCGC NspV 21 TTCGAA TT^CGAA PaeR7I 5087 CTCGAG C^TCGAG PinAI 1131 ACCGGT A^CCGGT SacI 3847 GAGCTC GAGCT^C SalI 359 GTCGAC G^TCGAC SapI 902 GCTCTTC GCTCTTC(1/4) ScaI 188 AGTACT AGT^ACT SexAI 4896 ACCTGGT A^CC[AT]GGT SfiI 4033 GGCCGTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 21 TTCGAA TT^CGAA SspBI 4110 TGTACA T^GTACA Tsp509I 38 AATT ^AATT XbaI 3966 TCTAGA T^CTAGA XhoI 5087 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 3629 GGTACC G^GTACC 4893 GGTACC AflIII 1427 ACACGT A^C[GA][CT]GT 3803 ACACGT AocI 269 CCTCAGG CC^T[ACGT]AGG 802 CCTGAGG Asp718I 3629 GGTACC G^GTACC 4893 GGTACC BalI 183 TGGCCA TGG^CCA 5343 TGGCCA BbsI 3381 GAAGAC GAAGAC(2/6) 4512 GAAGAC BciVI 1135 GTATCC GTATCC(6/5) 5080 GTATCC BpuAI 3381 GAAGAC GAAGAC(2/6) 4512 GAAGAC BspHI 104 TCATGA T^CATGA 3843 TCATGA BstAPI 1050 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2566 GCAGCCAGTGC Bsu36I 269 CCTCAGG CC^T[ACGT]AGG 802 CCTGAGG EagI 3323 CGGCCG C^GGCCG 5577 CGGCCG EclXI 3323 CGGCCG C^GGCCG 5577 CGGCCG Eco52I 3323 CGGCCG C^GGCCG 5577 CGGCCG Eco81I 269 CCTCAGG CC^T[ACGT]AGG 802 CCTGAGG EcoNI 1176 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1716 CCTCTCTAAGG EcoT22I 2408 ATGCAT ATGCA^T 3885 ATGCAT HpaI 2820 GTTAAC GTT^AAC 2858 GTTAAC KpnI 3629 GGTACC GGTAC^C 4893 GGTACC KspI 31 CCGCGG CCGC^GG 3227 CCGCGG MluNI 183 TGGCCA TGG^CCA 5343 TGGCCA MscI 183 TGGCCA TGG^CCA 5343 TGGCCA NsiI 2408 ATGCAT ATGCA^T 3885 ATGCAT RcaI 104 TCATGA T^CATGA 3843 TCATGA SacII 31 CCGCGG CCGC^GG 3227 CCGCGG SmaI 1566 CCCGGG CCC^GGG 3221 CCCGGG TfiI 1670 GAATC G^A[AT]TC 1768 GATTC XmaCI 1566 CCCGGG C^CCGGG 3221 CCCGGG XmaI 1566 CCCGGG C^CCGGG 3221 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII AviII AvrII BanI BanII BbvCI BbvI BcnI BfaI BglI BlnI BmyI BpmI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII