##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AclI AflII AscI
AseI AsnI Asp700I AspI BbeI
BbrPI BcgI BfrI BglII BlpI
Bpu1102I BsaAI BsaBI BseAI BsiWI
BsmI BspEI BspLU11I BssHII Bst1107I
BstZ17I CelII CpoI DraIII EcoRI
EcoRV FseI KasI MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NruI PacI PflFI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SbfI SfoI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 37 AAATTT [GA]^AATT[CT]
AgeI 1131 ACCGGT A^CCGGT
AhdI 5223 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4777 GTGCAC G^TGCAC
Aor51HI 2505 AGCGCT AGC^GCT
ApaLI 4777 GTGCAC G^TGCAC
ApoI 37 AAATTT [GA]^AATT[CT]
AspEI 5223 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 5265 GGATCC G^GATCC
BclI 4978 TGATCA T^GATCA
BsaI 1386 GGTCTC GGTCTC(1/5)
BsmBI 82 GAGACG CGTCTC(1/5)
Bsp1407I 4110 TGTACA T^GTACA
BspDI 1309 ATCGAT AT^CGAT
BsrBI 1611 GAGCGG CCGCTC(-3/-3)
BsrGI 4110 TGTACA T^GTACA
BstBI 21 TTCGAA TT^CGAA
ClaI 1309 ATCGAT AT^CGAT
DraI 2851 TTTAAA TTT^AAA
DrdI 1695 GACAGGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5223 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2505 AGCGCT AGC^GCT
FbaI 4978 TGATCA T^GATCA
HindIII 1997 AAGCTT A^AGCTT
NdeI 1831 CATATG CA^TATG
NotI 3322 GCGGCCGC GC^GGCCGC
NspV 21 TTCGAA TT^CGAA
PaeR7I 5087 CTCGAG C^TCGAG
PinAI 1131 ACCGGT A^CCGGT
SacI 3847 GAGCTC GAGCT^C
SalI 359 GTCGAC G^TCGAC
SapI 902 GCTCTTC GCTCTTC(1/4)
ScaI 188 AGTACT AGT^ACT
SexAI 4896 ACCTGGT A^CC[AT]GGT
SfiI 4033 GGCCGTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 21 TTCGAA TT^CGAA
SspBI 4110 TGTACA T^GTACA
Tsp509I 38 AATT ^AATT
XbaI 3966 TCTAGA T^CTAGA
XhoI 5087 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 3629 GGTACC G^GTACC
4893 GGTACC
AflIII 1427 ACACGT A^C[GA][CT]GT
3803 ACACGT
AocI 269 CCTCAGG CC^T[ACGT]AGG
802 CCTGAGG
Asp718I 3629 GGTACC G^GTACC
4893 GGTACC
BalI 183 TGGCCA TGG^CCA
5343 TGGCCA
BbsI 3381 GAAGAC GAAGAC(2/6)
4512 GAAGAC
BciVI 1135 GTATCC GTATCC(6/5)
5080 GTATCC
BpuAI 3381 GAAGAC GAAGAC(2/6)
4512 GAAGAC
BspHI 104 TCATGA T^CATGA
3843 TCATGA
BstAPI 1050 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2566 GCAGCCAGTGC
Bsu36I 269 CCTCAGG CC^T[ACGT]AGG
802 CCTGAGG
EagI 3323 CGGCCG C^GGCCG
5577 CGGCCG
EclXI 3323 CGGCCG C^GGCCG
5577 CGGCCG
Eco52I 3323 CGGCCG C^GGCCG
5577 CGGCCG
Eco81I 269 CCTCAGG CC^T[ACGT]AGG
802 CCTGAGG
EcoNI 1176 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1716 CCTCTCTAAGG
EcoT22I 2408 ATGCAT ATGCA^T
3885 ATGCAT
HpaI 2820 GTTAAC GTT^AAC
2858 GTTAAC
KpnI 3629 GGTACC GGTAC^C
4893 GGTACC
KspI 31 CCGCGG CCGC^GG
3227 CCGCGG
MluNI 183 TGGCCA TGG^CCA
5343 TGGCCA
MscI 183 TGGCCA TGG^CCA
5343 TGGCCA
NsiI 2408 ATGCAT ATGCA^T
3885 ATGCAT
RcaI 104 TCATGA T^CATGA
3843 TCATGA
SacII 31 CCGCGG CCGC^GG
3227 CCGCGG
SmaI 1566 CCCGGG CCC^GGG
3221 CCCGGG
TfiI 1670 GAATC G^A[AT]TC
1768 GATTC
XmaCI 1566 CCCGGG C^CCGGG
3221 CCCGGG
XmaI 1566 CCCGGG C^CCGGG
3221 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII AviII AvrII BanI
BanII BbvCI BbvI BcnI BfaI
BglI BlnI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BssSI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII