##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AcyI AflII AflIII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbrPI BfrI BlnI
BsaAI BsaBI BsaHI BsiEI BsiWI
BspDI BspLU11I BsrBI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoRI
FseI FspI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NarI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacII SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 1112 GTCGAC GT^[AC][GT]AC
AccIII 2959 TCCGGA T^CCGGA
AclI 3990 AACGTT AA^CGTT
Aor51HI 4286 AGCGCT AGC^GCT
Asp700I 1725 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1015 CCCGAG C^[CT]CG[GA]G
BamHI 1664 GGATCC G^GATCC
BcgI 365 CGATGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3391 TGATCA T^GATCA
BglI 381 GCCTGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 520 AGATCT A^GATCT
BsaWI 2959 TCCGGA [AT]^CCGG[AT]
BseAI 2959 TCCGGA T^CCGGA
BsmBI 906 GAGACG CGTCTC(1/5)
BsmI 436 GCATTC GAATGC(1/-1)
BsoBI 1015 CCCGAG C^[CT]CG[GA]G
BspEI 2959 TCCGGA T^CCGGA
BssHII 4777 GCGCGC G^CGCGC
BstEII 65 GGTGACC G^GT[ACGT]ACC
BstPI 65 GGTGACC G^GT[ACGT]ACC
Eco47III 4286 AGCGCT AGC^GCT
EcoO65I 65 GGTGACC G^GT[ACGT]ACC
EcoT22I 4836 ATGCAT ATGCA^T
FbaI 3391 TGATCA T^GATCA
MroI 2959 TCCGGA T^CCGGA
NaeI 2908 GCCGGC GCC^GGC
NcoI 497 CCATGG C^CATGG
NdeI 2389 CATATG CA^TATG
NgoMIV 2908 GCCGGC G^CCGGC
NheI 57 GCTAGC G^CTAGC
NsiI 4836 ATGCAT ATGCA^T
Psp1406I 3990 AACGTT AA^CGTT
SacI 4273 GAGCTC GAGCT^C
SalI 1112 GTCGAC G^TCGAC
SapI 1643 GAAGAGC GCTCTTC(1/4)
SbfI 2711 CCTGCAGG CCTGCA^GG
ScaI 3406 AGTACT AGT^ACT
Sse8387I 2711 CCTGCAGG CCTGCA^GG
SspI 1256 AATATT AAT^ATT
XbaI 893 TCTAGA T^CTAGA
XmnI 1725 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 1737 AAATTT [GA]^AATT[CT]
5054 GAATTT
ApoI 1737 AAATTT [GA]^AATT[CT]
5054 GAATTT
AspI 1130 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4640 GACACTGTC
BalI 2368 TGGCCA TGG^CCA
2818 TGGCCA
BciVI 956 GGATAC GTATCC(6/5)
3848 GTATCC
BspHI 1955 TCATGA T^CATGA
3388 TCATGA
BsrDI 371 CATTGC GCAATG(2/0)
490 GCAATG
BssSI 1893 CTCGTG CACGAG(-5/-1)
3807 CTCGTG
DraI 1260 TTTAAA TTT^AAA
5058 TTTAAA
EcoRV 2940 GATATC GAT^ATC
5212 GATATC
HaeII 678 AGCGCC [GA]GCGC^[CT]
4286 AGCGCT
HindIII 3538 AAGCTT A^AGCTT
4783 AAGCTT
MluNI 2368 TGGCCA TGG^CCA
2818 TGGCCA
MscI 2368 TGGCCA TGG^CCA
2818 TGGCCA
PflFI 1130 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4640 GACACTGTC
RcaI 1955 TCATGA T^CATGA
3388 TCATGA
SexAI 3136 ACCAGGT A^CC[AT]GGT
4174 ACCTGGT
Tth111I 1130 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4640 GACACTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrFI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII