################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII Acc65I AcyI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BbeI BbrPI BfrI BlnI BsaAI BsaBI BsaHI BsiEI BsiWI BspDI BspLU11I BsrBI Bst1107I BstBI BstXI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoRI FseI FspI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MunI NarI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 1112 GTCGAC GT^[AC][GT]AC AccIII 2959 TCCGGA T^CCGGA AclI 3990 AACGTT AA^CGTT Aor51HI 4286 AGCGCT AGC^GCT Asp700I 1725 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1015 CCCGAG C^[CT]CG[GA]G BamHI 1664 GGATCC G^GATCC BcgI 365 CGATGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 3391 TGATCA T^GATCA BglI 381 GCCTGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 520 AGATCT A^GATCT BsaWI 2959 TCCGGA [AT]^CCGG[AT] BseAI 2959 TCCGGA T^CCGGA BsmBI 906 GAGACG CGTCTC(1/5) BsmI 436 GCATTC GAATGC(1/-1) BsoBI 1015 CCCGAG C^[CT]CG[GA]G BspEI 2959 TCCGGA T^CCGGA BssHII 4777 GCGCGC G^CGCGC BstEII 65 GGTGACC G^GT[ACGT]ACC BstPI 65 GGTGACC G^GT[ACGT]ACC Eco47III 4286 AGCGCT AGC^GCT EcoO65I 65 GGTGACC G^GT[ACGT]ACC EcoT22I 4836 ATGCAT ATGCA^T FbaI 3391 TGATCA T^GATCA MroI 2959 TCCGGA T^CCGGA NaeI 2908 GCCGGC GCC^GGC NcoI 497 CCATGG C^CATGG NdeI 2389 CATATG CA^TATG NgoMIV 2908 GCCGGC G^CCGGC NheI 57 GCTAGC G^CTAGC NsiI 4836 ATGCAT ATGCA^T Psp1406I 3990 AACGTT AA^CGTT SacI 4273 GAGCTC GAGCT^C SalI 1112 GTCGAC G^TCGAC SapI 1643 GAAGAGC GCTCTTC(1/4) SbfI 2711 CCTGCAGG CCTGCA^GG ScaI 3406 AGTACT AGT^ACT Sse8387I 2711 CCTGCAGG CCTGCA^GG SspI 1256 AATATT AAT^ATT XbaI 893 TCTAGA T^CTAGA XmnI 1725 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 1737 AAATTT [GA]^AATT[CT] 5054 GAATTT ApoI 1737 AAATTT [GA]^AATT[CT] 5054 GAATTT AspI 1130 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4640 GACACTGTC BalI 2368 TGGCCA TGG^CCA 2818 TGGCCA BciVI 956 GGATAC GTATCC(6/5) 3848 GTATCC BspHI 1955 TCATGA T^CATGA 3388 TCATGA BsrDI 371 CATTGC GCAATG(2/0) 490 GCAATG BssSI 1893 CTCGTG CACGAG(-5/-1) 3807 CTCGTG DraI 1260 TTTAAA TTT^AAA 5058 TTTAAA EcoRV 2940 GATATC GAT^ATC 5212 GATATC HaeII 678 AGCGCC [GA]GCGC^[CT] 4286 AGCGCT HindIII 3538 AAGCTT A^AGCTT 4783 AAGCTT MluNI 2368 TGGCCA TGG^CCA 2818 TGGCCA MscI 2368 TGGCCA TGG^CCA 2818 TGGCCA PflFI 1130 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4640 GACACTGTC RcaI 1955 TCATGA T^CATGA 3388 TCATGA SexAI 3136 ACCAGGT A^CC[AT]GGT 4174 ACCTGGT Tth111I 1130 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4640 GACACTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII