##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AclI AcyI AflII Alw44I
ApaLI AscI AseI AsnI AspI
AvrII BbeI BcgI BciVI BfrI
BglII BlnI BsaBI BsaHI BsiEI
BsiWI Bsp1407I BspDI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoRV Hin1I HincII HindII
HpaI KasI KspI MamI MfeI
MluI MunI NarI NdeI NotI
NruI NspV PacI PaeR7I PflFI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SexAI
SfoI SfuI SgrAI SnaBI SphI
SspBI SspI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 2757 GTAGAC GT^[AC][GT]AC
AccIII 1809 TCCGGA T^CCGGA
AcsI 4947 GAATTC [GA]^AATT[CT]
AgeI 4063 ACCGGT A^CCGGT
ApoI 4947 GAATTC [GA]^AATT[CT]
Asp700I 1096 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 4837 TGGCCA TGG^CCA
BamHI 5041 GGATCC G^GATCC
BbrPI 4618 CACGTG CAC^GTG
BclI 3817 TGATCA T^GATCA
BseAI 1809 TCCGGA T^CCGGA
BsmBI 3912 GAGACG CGTCTC(1/5)
BspEI 1809 TCCGGA T^CCGGA
BsrDI 112 GCAATG GCAATG(2/0)
BstEII 4798 GGTGACC G^GT[ACGT]ACC
BstPI 4798 GGTGACC G^GT[ACGT]ACC
EcoO65I 4798 GGTGACC G^GT[ACGT]ACC
EcoRI 4947 GAATTC G^AATTC
EcoT22I 3255 ATGCAT ATGCA^T
FbaI 3817 TGATCA T^GATCA
FseI 2303 GGCCGGCC GGCCGG^CC
HindIII 2854 AAGCTT A^AGCTT
MluNI 4837 TGGCCA TGG^CCA
MroI 1809 TCCGGA T^CCGGA
MscI 4837 TGGCCA TGG^CCA
NheI 615 GCTAGC G^CTAGC
NsiI 3255 ATGCAT ATGCA^T
PinAI 4063 ACCGGT A^CCGGT
PmaCI 4618 CACGTG CAC^GTG
PmlI 4618 CACGTG CAC^GTG
SapI 3479 GAAGAGC GCTCTTC(1/4)
SbfI 401 CCTGCAGG CCTGCA^GG
SfiI 1158 GGCCGGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 5114 ACTAGT A^CTAGT
Sse8387I 401 CCTGCAGG CCTGCA^GG
XbaI 2219 TCTAGA T^CTAGA
XmnI 1096 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 1946 CGCG CG^CG
2280 CGCG
AocI 1323 CCTGAGG CC^T[ACGT]AGG
2947 CCTCAGG
BbvCI 5277 CCTCAGC CCTCAGC(-5/-2)
837 GCTGAGG
BsmI 1896 GAATGC GAATGC(1/-1)
2447 GAATGC
BspHI 770 TCATGA T^CATGA
4153 TCATGA
BspLU11I 3403 ACATGT A^CATGT
3779 ACATGT
BstUI 1946 CGCG CG^CG
2280 CGCG
Bsu36I 1323 CCTGAGG CC^T[ACGT]AGG
2947 CCTCAGG
Eco81I 1323 CCTGAGG CC^T[ACGT]AGG
2947 CCTCAGG
EcoNI 2622 CCTTCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3566 CCTGGAGGAGG
MvnI 1946 CGCG CG^CG
2280 CGCG
NaeI 2304 GCCGGC GCC^GGC
3484 GCCGGC
NgoMIV 2304 GCCGGC G^CCGGC
3484 GCCGGC
RcaI 770 TCATGA T^CATGA
4153 TCATGA
SacI 383 GAGCTC GAGCT^C
4355 GAGCTC
SmaI 343 CCCGGG CCC^GGG
2437 CCCGGG
XmaCI 343 CCCGGG C^CCGGG
2437 CCCGGG
XmaI 343 CCCGGG C^CCGGG
2437 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
Acc65I AciI AfaI AflIII AhdI
AluI AlwI AlwNI Aor51HI ApaI
Asp718I AspEI AspHI AvaI AvaII
AviII BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eam1105I EarI Eco47III
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII