##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BamHI BbeI BcgI BlnI
BsaI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BssHII BstBI BstEII
BstPI BstUI ClaI CpoI DrdI
Eam1105I EcoNI EcoO65I EcoT22I FseI
FspI KasI KspI MluI MroI
MvnI NaeI NarI NgoMIV NotI
NruI NsiI NspV PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 3869 GGTACC G^GTACC
AccI 4310 GTATAC GT^[AC][GT]AC
Aor51HI 921 AGCGCT AGC^GCT
Asp718I 3869 GGTACC G^GTACC
AvaI 3711 CTCGGG C^[CT]CG[GA]G
BbrPI 3912 CACGTG CAC^GTG
BbvCI 1390 CCTCAGC CCTCAGC(-5/-2)
BciVI 124 GTATCC GTATCC(6/5)
BclI 2425 TGATCA T^GATCA
BcnI 1592 CCCGG CC^[GC]GG
BglI 3823 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4881 AGATCT A^GATCT
BsaAI 3912 CACGTG [CT]AC^GT[GA]
BsaBI 4758 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 2188 GAGGAG GAGGAG(10/8)
BsoBI 3711 CTCGGG C^[CT]CG[GA]G
BsrBI 905 GAGCGG CCGCTC(-3/-3)
BsrFI 594 ACCGGC [GA]^CCGG[CT]
BssSI 3128 CACGAG CACGAG(-5/-1)
Bst1107I 4310 GTATAC GTA^TAC
BstZ17I 4310 GTATAC GTA^TAC
Cfr10I 594 ACCGGC [GA]^CCGG[CT]
EagI 409 CGGCCG C^GGCCG
EclXI 409 CGGCCG C^GGCCG
Eco47III 921 AGCGCT AGC^GCT
Eco52I 409 CGGCCG C^GGCCG
EcoRI 850 GAATTC G^AATTC
EcoRV 3859 GATATC GAT^ATC
FbaI 2425 TGATCA T^GATCA
KpnI 3869 GGTACC GGTAC^C
MamI 4758 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1847 CAATTG C^AATTG
MunI 1847 CAATTG C^AATTG
NciI 1592 CCCGG CC^[GC]GG
NdeI 2512 CATATG CA^TATG
NheI 785 GCTAGC G^CTAGC
PacI 4127 TTAATTAA TTAAT^TAA
PmaCI 3912 CACGTG CAC^GTG
PmlI 3912 CACGTG CAC^GTG
PpuMI 546 AGGACCC [GA]G^G[AT]CC[CT]
SphI 1523 GCATGC GCATG^C
TaqI 385 TCGA T^CGA
TthHB8I 385 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflII 2796 CTTAAG C^TTAAG
3052 CTTAAG
AocI 3536 CCTCAGG CC^T[ACGT]AGG
4297 CCTCAGG
Asp700I 1072 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1431 GAAGAAATTC
BfrI 2796 CTTAAG C^TTAAG
3052 CTTAAG
BsgI 1282 CTGCAC GTGCAG(16/14)
4493 GTGCAG
BsiEI 409 CGGCCG CG[GA][CT]^CG
1237 CGGTCG
BspHI 2947 TCATGA T^CATGA
3313 TCATGA
BspLU11I 4335 ACATGT A^CATGT
4554 ACATGT
BspMI 757 ACCTGC ACCTGC(4/8)
2931 ACCTGC
BsrDI 3722 CATTGC GCAATG(2/0)
3037 GCAATG
BstXI 1246 CCACTTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1730 CCAGTGTTTTGG
BstYI 444 AGATCC [GA]^GATC[CT]
4881 AGATCT
Bsu36I 3536 CCTCAGG CC^T[ACGT]AGG
4297 CCTCAGG
DraIII 3108 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4852 CACATGGTG
Eco81I 3536 CCTCAGG CC^T[ACGT]AGG
4297 CCTCAGG
HaeII 921 AGCGCT [GA]GCGC^[CT]
1556 GGCGCT
HapII 595 CCGG C^CGG
1593 CCGG
HpaI 2143 GTTAAC GTT^AAC
2901 GTTAAC
HpaII 595 CCGG C^CGG
1593 CCGG
MaeII 831 ACGT A^CGT
3913 ACGT
MflI 444 AGATCC [GA]^GATC[CT]
4881 AGATCT
MspI 595 CCGG C^CGG
1593 CCGG
PflMI 3213 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4851 CCACATGGTGG
PshAI 706 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4409 GACAAATGTC
PstI 3905 CTGCAG CTGCA^G
4753 CTGCAG
RcaI 2947 TCATGA T^CATGA
3313 TCATGA
SacI 1620 GAGCTC GAGCT^C
3572 GAGCTC
SapI 1350 GCTCTTC GCTCTTC(1/4)
4816 GCTCTTC
SexAI 270 ACCAGGT A^CC[AT]GGT
4305 ACCAGGT
SpeI 3010 ACTAGT A^CTAGT
3387 ACTAGT
StuI 553 AGGCCT AGG^CCT
3612 AGGCCT
Van91I 3213 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4851 CCACATGGTGG
XhoII 444 AGATCC [GA]^GATC[CT]
4881 AGATCT
XmnI 1072 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1431 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstAPI BstF5I BstNI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI