##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AcyI AflII AgeI
AhdI Aor51HI AscI AspEI AspI
AviII BbeI BcgI BfrI BsaHI
BseAI BsiWI BspDI BspEI BssHII
BssSI BstEII BstPI ClaI CpoI
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
FauI FseI FspI Hin1I HpaI
KasI KspI MluI MroI NarI
NcoI NotI NruI PflFI PflMI
PinAI PmeI PshAI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SmaI SnaBI Sse8387I
StuI Tth111I Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 83 GGTACC G^GTACC
AccII 1537 CGCG CG^CG
AclI 3136 AACGTT AA^CGTT
AocI 2663 CCTTAGG CC^T[ACGT]AGG
ApaI 2433 GGGCCC GGGCC^C
Asp718I 83 GGTACC G^GTACC
AvaI 109 CTCGAG C^[CT]CG[GA]G
AvrII 3883 CCTAGG C^CTAGG
BamHI 1959 GGATCC G^GATCC
BbrPI 3835 CACGTG CAC^GTG
BciVI 3118 GTATCC GTATCC(6/5)
BglI 3454 GCCCTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3883 CCTAGG C^CTAGG
BsaAI 3835 CACGTG [CT]AC^GT[GA]
BsaBI 1111 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1338 ACCGGA [AT]^CCGG[AT]
BsiEI 4725 CGATCG CG[GA][CT]^CG
BsmBI 1970 CGTCTC CGTCTC(1/5)
BsoBI 109 CTCGAG C^[CT]CG[GA]G
BspHI 279 TCATGA T^CATGA
BstBI 1433 TTCGAA TT^CGAA
BstUI 1537 CGCG CG^CG
Bsu36I 2663 CCTTAGG CC^T[ACGT]AGG
Eco81I 2663 CCTTAGG CC^T[ACGT]AGG
EcoRV 3125 GATATC GAT^ATC
HgaI 4721 GCGTC GACGC(5/10)
KpnI 83 GGTACC GGTAC^C
MamI 1111 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2221 CAATTG C^AATTG
MunI 2221 CAATTG C^AATTG
MvnI 1537 CGCG CG^CG
NaeI 3459 GCCGGC GCC^GGC
NgoMIV 3459 GCCGGC G^CCGGC
NheI 396 GCTAGC G^CTAGC
NspV 1433 TTCGAA TT^CGAA
PacI 4551 TTAATTAA TTAAT^TAA
PaeR7I 109 CTCGAG C^TCGAG
PmaCI 3835 CACGTG CAC^GTG
PmlI 3835 CACGTG CAC^GTG
PpuMI 1085 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 3136 AACGTT AA^CGTT
PvuI 4725 CGATCG CGAT^CG
RcaI 279 TCATGA T^CATGA
SfuI 1433 TTCGAA TT^CGAA
SgrAI 616 CACCGGCG C[GA]^CCGG[CT]G
SpeI 2103 ACTAGT A^CTAGT
SphI 3336 GCATGC GCATG^C
SwaI 794 ATTTAAAT ATTT^AAAT
XbaI 2653 TCTAGA T^CTAGA
XhoI 109 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AccI 2867 GTATAC GT^[AC][GT]AC
3593 GTATAC
Alw44I 3844 GTGCAC G^TGCAC
3965 GTGCAC
ApaLI 3844 GTGCAC G^TGCAC
3965 GTGCAC
AseI 2696 ATTAAT AT^TAAT
4065 ATTAAT
AsnI 2696 ATTAAT AT^TAAT
4065 ATTAAT
AvaII 1086 GGTCC G^G[AT]CC
3892 GGTCC
BalI 3522 TGGCCA TGG^CCA
3831 TGGCCA
BanI 83 GGTACC G^G[CT][GA]CC
4040 GGTGCC
BanII 2414 GGGCTC G[GA]GC[CT]^C
2433 GGGCCC
BbvCI 3514 CCTCAGC CCTCAGC(-5/-2)
2060 GCTGAGG
BclI 576 TGATCA T^GATCA
4311 TGATCA
BcnI 87 CCGGG CC^[GC]GG
846 CCCGG
BglII 1788 AGATCT A^GATCT
4495 AGATCT
BlpI 400 GCTGAGC GC^T[ACGT]AGC
1141 GCTCAGC
Bpu1102I 400 GCTGAGC GC^T[ACGT]AGC
1141 GCTCAGC
BsaI 470 GGTCTC GGTCTC(1/5)
1992 GGTCTC
BseRI 4753 CTCCTC GAGGAG(10/8)
637 GAGGAG
BspLU11I 410 ACATGT A^CATGT
3628 ACATGT
BspMI 2200 GCAGGT ACCTGC(4/8)
4330 GCAGGT
BsrBI 4737 CCGCTC CCGCTC(-3/-3)
1508 GAGCGG
BsrDI 2077 GCAATG GCAATG(2/0)
4359 GCAATG
BsrFI 617 ACCGGC [GA]^CCGG[CT]
3459 GCCGGC
Bst1107I 2867 GTATAC GTA^TAC
3593 GTATAC
BstXI 129 CCAGAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3178 CCAGCTCTTTGG
BstZ17I 2867 GTATAC GTA^TAC
3593 GTATAC
CelII 400 GCTGAGC GC^T[ACGT]AGC
1141 GCTCAGC
Cfr10I 617 ACCGGC [GA]^CCGG[CT]
3459 GCCGGC
DrdI 1014 GACAAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4162 GACTTAAATGTC
EaeI 3522 TGGCCA [CT]^GGCC[GA]
3831 TGGCCA
EarI 58 CTCTTC CTCTTC(1/4)
1995 CTCTTC
EcoT14I 3883 CCTAGG C^C[AT][AT]GG
4762 CCAAGG
FbaI 576 TGATCA T^GATCA
4311 TGATCA
HaeII 849 GGCGCT [GA]GCGC^[CT]
1710 AGCGCC
HindIII 730 AAGCTT A^AGCTT
3798 AAGCTT
Ksp632I 58 CTCTTC CTCTTC(1/4)
1995 CTCTTC
MluNI 3522 TGGCCA TGG^CCA
3831 TGGCCA
MscI 3522 TGGCCA TGG^CCA
3831 TGGCCA
MspA1I 182 CAGCTG C[AC]G^C[GT]G
3896 CAGCTG
NciI 87 CCGGG CC^[GC]GG
846 CCCGG
PshBI 2696 ATTAAT AT^TAAT
4065 ATTAAT
PstI 2116 CTGCAG CTGCA^G
4328 CTGCAG
PvuII 182 CAGCTG CAG^CTG
3896 CAGCTG
ScaI 1702 AGTACT AGT^ACT
2608 AGTACT
StyI 3883 CCTAGG C^C[AT][AT]GG
4762 CCAAGG
XcmI 2447 CCACCTCTCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4209 CCATGCAGTTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EcoO109I EcoRII EcoT22I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NsiI NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI