##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       AccIII      AcyI        AflII       AgeI        
AhdI        Aor51HI     AscI        AspEI       AspI        
AviII       BbeI        BcgI        BfrI        BsaHI       
BseAI       BsiWI       BspDI       BspEI       BssHII      
BssSI       BstEII      BstPI       ClaI        CpoI        
DraIII      DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
FauI        FseI        FspI        Hin1I       HpaI        
KasI        KspI        MluI        MroI        NarI        
NcoI        NotI        NruI        PflFI       PflMI       
PinAI       PmeI        PshAI       RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SmaI        SnaBI       Sse8387I    
StuI        Tth111I     Van91I      XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I         83  GGTACC               G^GTACC
AccII        1537  CGCG                 CG^CG
AclI         3136  AACGTT               AA^CGTT
AocI         2663  CCTTAGG              CC^T[ACGT]AGG
ApaI         2433  GGGCCC               GGGCC^C
Asp718I        83  GGTACC               G^GTACC
AvaI          109  CTCGAG               C^[CT]CG[GA]G
AvrII        3883  CCTAGG               C^CTAGG
BamHI        1959  GGATCC               G^GATCC
BbrPI        3835  CACGTG               CAC^GTG
BciVI        3118  GTATCC               GTATCC(6/5)
BglI         3454  GCCCTGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         3883  CCTAGG               C^CTAGG
BsaAI        3835  CACGTG               [CT]AC^GT[GA]
BsaBI        1111  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1338  ACCGGA               [AT]^CCGG[AT]
BsiEI        4725  CGATCG               CG[GA][CT]^CG
BsmBI        1970  CGTCTC               CGTCTC(1/5)
BsoBI         109  CTCGAG               C^[CT]CG[GA]G
BspHI         279  TCATGA               T^CATGA
BstBI        1433  TTCGAA               TT^CGAA
BstUI        1537  CGCG                 CG^CG
Bsu36I       2663  CCTTAGG              CC^T[ACGT]AGG
Eco81I       2663  CCTTAGG              CC^T[ACGT]AGG
EcoRV        3125  GATATC               GAT^ATC
HgaI         4721  GCGTC                GACGC(5/10)
KpnI           83  GGTACC               GGTAC^C
MamI         1111  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2221  CAATTG               C^AATTG
MunI         2221  CAATTG               C^AATTG
MvnI         1537  CGCG                 CG^CG
NaeI         3459  GCCGGC               GCC^GGC
NgoMIV       3459  GCCGGC               G^CCGGC
NheI          396  GCTAGC               G^CTAGC
NspV         1433  TTCGAA               TT^CGAA
PacI         4551  TTAATTAA             TTAAT^TAA
PaeR7I        109  CTCGAG               C^TCGAG
PmaCI        3835  CACGTG               CAC^GTG
PmlI         3835  CACGTG               CAC^GTG
PpuMI        1085  AGGTCCC              [GA]G^G[AT]CC[CT]
Psp1406I     3136  AACGTT               AA^CGTT
PvuI         4725  CGATCG               CGAT^CG
RcaI          279  TCATGA               T^CATGA
SfuI         1433  TTCGAA               TT^CGAA
SgrAI         616  CACCGGCG             C[GA]^CCGG[CT]G
SpeI         2103  ACTAGT               A^CTAGT
SphI         3336  GCATGC               GCATG^C
SwaI          794  ATTTAAAT             ATTT^AAAT
XbaI         2653  TCTAGA               T^CTAGA
XhoI          109  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   45 enzymes) #
###################################

AccI         2867  GTATAC               GT^[AC][GT]AC
             3593  GTATAC              
Alw44I       3844  GTGCAC               G^TGCAC
             3965  GTGCAC              
ApaLI        3844  GTGCAC               G^TGCAC
             3965  GTGCAC              
AseI         2696  ATTAAT               AT^TAAT
             4065  ATTAAT              
AsnI         2696  ATTAAT               AT^TAAT
             4065  ATTAAT              
AvaII        1086  GGTCC                G^G[AT]CC
             3892  GGTCC               
BalI         3522  TGGCCA               TGG^CCA
             3831  TGGCCA              
BanI           83  GGTACC               G^G[CT][GA]CC
             4040  GGTGCC              
BanII        2414  GGGCTC               G[GA]GC[CT]^C
             2433  GGGCCC              
BbvCI        3514  CCTCAGC              CCTCAGC(-5/-2)
             2060  GCTGAGG             
BclI          576  TGATCA               T^GATCA
             4311  TGATCA              
BcnI           87  CCGGG                CC^[GC]GG
              846  CCCGG               
BglII        1788  AGATCT               A^GATCT
             4495  AGATCT              
BlpI          400  GCTGAGC              GC^T[ACGT]AGC
             1141  GCTCAGC             
Bpu1102I      400  GCTGAGC              GC^T[ACGT]AGC
             1141  GCTCAGC             
BsaI          470  GGTCTC               GGTCTC(1/5)
             1992  GGTCTC              
BseRI        4753  CTCCTC               GAGGAG(10/8)
              637  GAGGAG              
BspLU11I      410  ACATGT               A^CATGT
             3628  ACATGT              
BspMI        2200  GCAGGT               ACCTGC(4/8)
             4330  GCAGGT              
BsrBI        4737  CCGCTC               CCGCTC(-3/-3)
             1508  GAGCGG              
BsrDI        2077  GCAATG               GCAATG(2/0)
             4359  GCAATG              
BsrFI         617  ACCGGC               [GA]^CCGG[CT]
             3459  GCCGGC              
Bst1107I     2867  GTATAC               GTA^TAC
             3593  GTATAC              
BstXI         129  CCAGAGAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3178  CCAGCTCTTTGG        
BstZ17I      2867  GTATAC               GTA^TAC
             3593  GTATAC              
CelII         400  GCTGAGC              GC^T[ACGT]AGC
             1141  GCTCAGC             
Cfr10I        617  ACCGGC               [GA]^CCGG[CT]
             3459  GCCGGC              
DrdI         1014  GACAAAAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4162  GACTTAAATGTC        
EaeI         3522  TGGCCA               [CT]^GGCC[GA]
             3831  TGGCCA              
EarI           58  CTCTTC               CTCTTC(1/4)
             1995  CTCTTC              
EcoT14I      3883  CCTAGG               C^C[AT][AT]GG
             4762  CCAAGG              
FbaI          576  TGATCA               T^GATCA
             4311  TGATCA              
HaeII         849  GGCGCT               [GA]GCGC^[CT]
             1710  AGCGCC              
HindIII       730  AAGCTT               A^AGCTT
             3798  AAGCTT              
Ksp632I        58  CTCTTC               CTCTTC(1/4)
             1995  CTCTTC              
MluNI        3522  TGGCCA               TGG^CCA
             3831  TGGCCA              
MscI         3522  TGGCCA               TGG^CCA
             3831  TGGCCA              
MspA1I        182  CAGCTG               C[AC]G^C[GT]G
             3896  CAGCTG              
NciI           87  CCGGG                CC^[GC]GG
              846  CCCGG               
PshBI        2696  ATTAAT               AT^TAAT
             4065  ATTAAT              
PstI         2116  CTGCAG               CTGCA^G
             4328  CTGCAG              
PvuII         182  CAGCTG               CAG^CTG
             3896  CAGCTG              
ScaI         1702  AGTACT               AGT^ACT
             2608  AGTACT              
StyI         3883  CCTAGG               C^C[AT][AT]GG
             4762  CCAAGG              
XcmI         2447  CCACCTCTCCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4209  CCATGCAGTTGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
BbsI        BbvI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
Bsp1407I    BsrGI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
EcoO109I    EcoRII      EcoT22I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspI        
MvaI        MwoI        NdeI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       SspI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI