################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII AccIII AcyI AflII AgeI AhdI Aor51HI AscI AspEI AspI AviII BbeI BcgI BfrI BsaHI BseAI BsiWI BspDI BspEI BssHII BssSI BstEII BstPI ClaI CpoI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FauI FseI FspI Hin1I HpaI KasI KspI MluI MroI NarI NcoI NotI NruI PflFI PflMI PinAI PmeI PshAI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SmaI SnaBI Sse8387I StuI Tth111I Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 83 GGTACC G^GTACC AccII 1537 CGCG CG^CG AclI 3136 AACGTT AA^CGTT AocI 2663 CCTTAGG CC^T[ACGT]AGG ApaI 2433 GGGCCC GGGCC^C Asp718I 83 GGTACC G^GTACC AvaI 109 CTCGAG C^[CT]CG[GA]G AvrII 3883 CCTAGG C^CTAGG BamHI 1959 GGATCC G^GATCC BbrPI 3835 CACGTG CAC^GTG BciVI 3118 GTATCC GTATCC(6/5) BglI 3454 GCCCTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 3883 CCTAGG C^CTAGG BsaAI 3835 CACGTG [CT]AC^GT[GA] BsaBI 1111 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1338 ACCGGA [AT]^CCGG[AT] BsiEI 4725 CGATCG CG[GA][CT]^CG BsmBI 1970 CGTCTC CGTCTC(1/5) BsoBI 109 CTCGAG C^[CT]CG[GA]G BspHI 279 TCATGA T^CATGA BstBI 1433 TTCGAA TT^CGAA BstUI 1537 CGCG CG^CG Bsu36I 2663 CCTTAGG CC^T[ACGT]AGG Eco81I 2663 CCTTAGG CC^T[ACGT]AGG EcoRV 3125 GATATC GAT^ATC HgaI 4721 GCGTC GACGC(5/10) KpnI 83 GGTACC GGTAC^C MamI 1111 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2221 CAATTG C^AATTG MunI 2221 CAATTG C^AATTG MvnI 1537 CGCG CG^CG NaeI 3459 GCCGGC GCC^GGC NgoMIV 3459 GCCGGC G^CCGGC NheI 396 GCTAGC G^CTAGC NspV 1433 TTCGAA TT^CGAA PacI 4551 TTAATTAA TTAAT^TAA PaeR7I 109 CTCGAG C^TCGAG PmaCI 3835 CACGTG CAC^GTG PmlI 3835 CACGTG CAC^GTG PpuMI 1085 AGGTCCC [GA]G^G[AT]CC[CT] Psp1406I 3136 AACGTT AA^CGTT PvuI 4725 CGATCG CGAT^CG RcaI 279 TCATGA T^CATGA SfuI 1433 TTCGAA TT^CGAA SgrAI 616 CACCGGCG C[GA]^CCGG[CT]G SpeI 2103 ACTAGT A^CTAGT SphI 3336 GCATGC GCATG^C SwaI 794 ATTTAAAT ATTT^AAAT XbaI 2653 TCTAGA T^CTAGA XhoI 109 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 45 enzymes) # ################################### AccI 2867 GTATAC GT^[AC][GT]AC 3593 GTATAC Alw44I 3844 GTGCAC G^TGCAC 3965 GTGCAC ApaLI 3844 GTGCAC G^TGCAC 3965 GTGCAC AseI 2696 ATTAAT AT^TAAT 4065 ATTAAT AsnI 2696 ATTAAT AT^TAAT 4065 ATTAAT AvaII 1086 GGTCC G^G[AT]CC 3892 GGTCC BalI 3522 TGGCCA TGG^CCA 3831 TGGCCA BanI 83 GGTACC G^G[CT][GA]CC 4040 GGTGCC BanII 2414 GGGCTC G[GA]GC[CT]^C 2433 GGGCCC BbvCI 3514 CCTCAGC CCTCAGC(-5/-2) 2060 GCTGAGG BclI 576 TGATCA T^GATCA 4311 TGATCA BcnI 87 CCGGG CC^[GC]GG 846 CCCGG BglII 1788 AGATCT A^GATCT 4495 AGATCT BlpI 400 GCTGAGC GC^T[ACGT]AGC 1141 GCTCAGC Bpu1102I 400 GCTGAGC GC^T[ACGT]AGC 1141 GCTCAGC BsaI 470 GGTCTC GGTCTC(1/5) 1992 GGTCTC BseRI 4753 CTCCTC GAGGAG(10/8) 637 GAGGAG BspLU11I 410 ACATGT A^CATGT 3628 ACATGT BspMI 2200 GCAGGT ACCTGC(4/8) 4330 GCAGGT BsrBI 4737 CCGCTC CCGCTC(-3/-3) 1508 GAGCGG BsrDI 2077 GCAATG GCAATG(2/0) 4359 GCAATG BsrFI 617 ACCGGC [GA]^CCGG[CT] 3459 GCCGGC Bst1107I 2867 GTATAC GTA^TAC 3593 GTATAC BstXI 129 CCAGAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3178 CCAGCTCTTTGG BstZ17I 2867 GTATAC GTA^TAC 3593 GTATAC CelII 400 GCTGAGC GC^T[ACGT]AGC 1141 GCTCAGC Cfr10I 617 ACCGGC [GA]^CCGG[CT] 3459 GCCGGC DrdI 1014 GACAAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4162 GACTTAAATGTC EaeI 3522 TGGCCA [CT]^GGCC[GA] 3831 TGGCCA EarI 58 CTCTTC CTCTTC(1/4) 1995 CTCTTC EcoT14I 3883 CCTAGG C^C[AT][AT]GG 4762 CCAAGG FbaI 576 TGATCA T^GATCA 4311 TGATCA HaeII 849 GGCGCT [GA]GCGC^[CT] 1710 AGCGCC HindIII 730 AAGCTT A^AGCTT 3798 AAGCTT Ksp632I 58 CTCTTC CTCTTC(1/4) 1995 CTCTTC MluNI 3522 TGGCCA TGG^CCA 3831 TGGCCA MscI 3522 TGGCCA TGG^CCA 3831 TGGCCA MspA1I 182 CAGCTG C[AC]G^C[GT]G 3896 CAGCTG NciI 87 CCGGG CC^[GC]GG 846 CCCGG PshBI 2696 ATTAAT AT^TAAT 4065 ATTAAT PstI 2116 CTGCAG CTGCA^G 4328 CTGCAG PvuII 182 CAGCTG CAG^CTG 3896 CAGCTG ScaI 1702 AGTACT AGT^ACT 2608 AGTACT StyI 3883 CCTAGG C^C[AT][AT]GG 4762 CCAAGG XcmI 2447 CCACCTCTCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4209 CCATGCAGTTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EcoO109I EcoRII EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeI NdeII NlaIII NlaIV NsiI NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI