##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AflII AgeI Alw44I ApaLI AscI
AvrII BfrI BlnI BsaBI BsiWI
BsmBI BssHII Bst1107I BstZ17I CpoI
EagI EclXI Eco52I EcoRI EcoRV
FseI HpaI KspI MamI MluI
NdeI NotI NruI PacI PinAI
PmeI RsrII SacII SalI SbfI
ScaI SfiI SgrAI SnaBI SpeI
Sse8387I StuI SwaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 3176 TCCGGA T^CCGGA
AclI 5153 AACGTT AA^CGTT
AhdI 387 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3924 AGCGCT AGC^GCT
AspEI 387 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3242 TGCGCA TGC^GCA
BbeI 2473 GGCGCC GGCGC^C
BglI 2651 GCCACCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 3176 TCCGGA T^CCGGA
BsiEI 2497 CGATCG CG[GA][CT]^CG
BspDI 2587 ATCGAT AT^CGAT
BspEI 3176 TCCGGA T^CCGGA
BsrBI 1897 GAGCGG CCGCTC(-3/-3)
BssSI 3976 CACGAG CACGAG(-5/-1)
ClaI 2587 ATCGAT AT^CGAT
Eam1105I 387 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 574 GAAGAG CTCTTC(1/4)
Eco47III 3924 AGCGCT AGC^GCT
FspI 3242 TGCGCA TGC^GCA
KasI 2473 GGCGCC G^GCGCC
Ksp632I 574 GAAGAG CTCTTC(1/4)
MroI 3176 TCCGGA T^CCGGA
NaeI 2776 GCCGGC GCC^GGC
NarI 2473 GGCGCC GG^CGCC
NgoMIV 2776 GCCGGC G^CCGGC
NheI 465 GCTAGC G^CTAGC
Psp1406I 5153 AACGTT AA^CGTT
PvuI 2497 CGATCG CGAT^CG
SacI 3271 GAGCTC GAGCT^C
SapI 574 GAAGAGC GCTCTTC(1/4)
SfoI 2473 GGCGCC GGC^GCC
SspI 5310 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 2526 GACGTC GACGT^C
3660 GACGTC
ApaI 3544 GGGCCC GGGCC^C
4788 GGGCCC
AseI 2200 ATTAAT AT^TAAT
6059 ATTAAT
AsnI 2200 ATTAAT AT^TAAT
6059 ATTAAT
AspI 2893 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4836 GACCATGTC
BbrPI 1089 CACGTG CAC^GTG
2097 CACGTG
BclI 2279 TGATCA T^GATCA
3086 TGATCA
BglII 60 AGATCT A^GATCT
6092 AGATCT
BsaWI 1841 TCCGGT [AT]^CCGG[AT]
3176 TCCGGA
Bsp1407I 209 TGTACA T^GTACA
1143 TGTACA
BsrGI 209 TGTACA T^GTACA
1143 TGTACA
BstBI 698 TTCGAA TT^CGAA
4596 TTCGAA
DraI 4545 TTTAAA TTT^AAA
4665 TTTAAA
DrdI 3019 GACCCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3373 GACTACAGAGTC
FbaI 2279 TGATCA T^GATCA
3086 TGATCA
HincII 1762 GTTGAC GT[CT]^[GA]AC
3184 GTCAAC
HindII 1762 GTTGAC GT[CT]^[GA]AC
3184 GTCAAC
HindIII 4202 AAGCTT A^AGCTT
5770 AAGCTT
NspV 698 TTCGAA TT^CGAA
4596 TTCGAA
PaeR7I 1019 CTCGAG C^TCGAG
2555 CTCGAG
PflFI 2893 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4836 GACCATGTC
PmaCI 1089 CACGTG CAC^GTG
2097 CACGTG
PmlI 1089 CACGTG CAC^GTG
2097 CACGTG
PshAI 1801 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5508 GACCATGGTC
PshBI 2200 ATTAAT AT^TAAT
6059 ATTAAT
SexAI 1295 ACCTGGT A^CC[AT]GGT
3521 ACCTGGT
SfuI 698 TTCGAA TT^CGAA
4596 TTCGAA
SphI 2735 GCATGC GCATG^C
2816 GCATGC
SspBI 209 TGTACA T^GTACA
1143 TGTACA
Tth111I 2893 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4836 GACCATGTC
XbaI 264 TCTAGA T^CTAGA
3870 TCTAGA
XhoI 1019 CTCGAG C^TCGAG
2555 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI AocI ApoI Asp700I Asp718I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcgI BciVI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspLU11I
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KpnI MaeI MaeII MaeIII
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI