################################## # NO CUT ENZYMES ( 43 enzymes) # ################################## AflII AgeI Alw44I ApaLI AscI AvrII BfrI BlnI BsaBI BsiWI BsmBI BssHII Bst1107I BstZ17I CpoI EagI EclXI Eco52I EcoRI EcoRV FseI HpaI KspI MamI MluI NdeI NotI NruI PacI PinAI PmeI RsrII SacII SalI SbfI ScaI SfiI SgrAI SnaBI SpeI Sse8387I StuI SwaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccIII 3176 TCCGGA T^CCGGA AclI 5153 AACGTT AA^CGTT AhdI 387 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3924 AGCGCT AGC^GCT AspEI 387 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3242 TGCGCA TGC^GCA BbeI 2473 GGCGCC GGCGC^C BglI 2651 GCCACCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseAI 3176 TCCGGA T^CCGGA BsiEI 2497 CGATCG CG[GA][CT]^CG BspDI 2587 ATCGAT AT^CGAT BspEI 3176 TCCGGA T^CCGGA BsrBI 1897 GAGCGG CCGCTC(-3/-3) BssSI 3976 CACGAG CACGAG(-5/-1) ClaI 2587 ATCGAT AT^CGAT Eam1105I 387 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 574 GAAGAG CTCTTC(1/4) Eco47III 3924 AGCGCT AGC^GCT FspI 3242 TGCGCA TGC^GCA KasI 2473 GGCGCC G^GCGCC Ksp632I 574 GAAGAG CTCTTC(1/4) MroI 3176 TCCGGA T^CCGGA NaeI 2776 GCCGGC GCC^GGC NarI 2473 GGCGCC GG^CGCC NgoMIV 2776 GCCGGC G^CCGGC NheI 465 GCTAGC G^CTAGC Psp1406I 5153 AACGTT AA^CGTT PvuI 2497 CGATCG CGAT^CG SacI 3271 GAGCTC GAGCT^C SapI 574 GAAGAGC GCTCTTC(1/4) SfoI 2473 GGCGCC GGC^GCC SspI 5310 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AatII 2526 GACGTC GACGT^C 3660 GACGTC ApaI 3544 GGGCCC GGGCC^C 4788 GGGCCC AseI 2200 ATTAAT AT^TAAT 6059 ATTAAT AsnI 2200 ATTAAT AT^TAAT 6059 ATTAAT AspI 2893 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4836 GACCATGTC BbrPI 1089 CACGTG CAC^GTG 2097 CACGTG BclI 2279 TGATCA T^GATCA 3086 TGATCA BglII 60 AGATCT A^GATCT 6092 AGATCT BsaWI 1841 TCCGGT [AT]^CCGG[AT] 3176 TCCGGA Bsp1407I 209 TGTACA T^GTACA 1143 TGTACA BsrGI 209 TGTACA T^GTACA 1143 TGTACA BstBI 698 TTCGAA TT^CGAA 4596 TTCGAA DraI 4545 TTTAAA TTT^AAA 4665 TTTAAA DrdI 3019 GACCCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3373 GACTACAGAGTC FbaI 2279 TGATCA T^GATCA 3086 TGATCA HincII 1762 GTTGAC GT[CT]^[GA]AC 3184 GTCAAC HindII 1762 GTTGAC GT[CT]^[GA]AC 3184 GTCAAC HindIII 4202 AAGCTT A^AGCTT 5770 AAGCTT NspV 698 TTCGAA TT^CGAA 4596 TTCGAA PaeR7I 1019 CTCGAG C^TCGAG 2555 CTCGAG PflFI 2893 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4836 GACCATGTC PmaCI 1089 CACGTG CAC^GTG 2097 CACGTG PmlI 1089 CACGTG CAC^GTG 2097 CACGTG PshAI 1801 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5508 GACCATGGTC PshBI 2200 ATTAAT AT^TAAT 6059 ATTAAT SexAI 1295 ACCTGGT A^CC[AT]GGT 3521 ACCTGGT SfuI 698 TTCGAA TT^CGAA 4596 TTCGAA SphI 2735 GCATGC GCATG^C 2816 GCATGC SspBI 209 TGTACA T^GTACA 1143 TGTACA Tth111I 2893 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4836 GACCATGTC XbaI 264 TCTAGA T^CTAGA 3870 TCTAGA XhoI 1019 CTCGAG C^TCGAG 2555 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 151 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I Asp718I AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcgI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KpnI MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI