##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccII AccIII AclI AflII AscI
AseI AsnI BcgI BclI BfrI
BlpI Bpu1102I BsaBI BseAI BsiEI
BsiWI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CelII
ClaI CpoI DrdI EagI EclXI
Eco52I EcoT22I FbaI FseI HincII
HindII HindIII HpaI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI ScaI SfiI SfuI
SgrAI SmaI SpeI SphI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 750 GACGTC GACGT^C
Acc65I 6111 GGTACC G^GTACC
AccI 5753 GTAGAC GT^[AC][GT]AC
AgeI 2527 ACCGGT A^CCGGT
AhdI 1922 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1781 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 4067 GGGCCC GGGCC^C
Asp700I 1564 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 6111 GGTACC G^GTACC
AspEI 1922 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2631 TGCGCA TGC^GCA
BamHI 1726 GGATCC G^GATCC
BcnI 2289 CCGGG CC^[GC]GG
BglI 3808 GCCCCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4300 AGATCT A^GATCT
BsaWI 2527 ACCGGT [AT]^CCGG[AT]
BsmBI 1996 CGTCTC CGTCTC(1/5)
BsmI 48 GAATGC GAATGC(1/-1)
BspMI 2246 GCAGGT ACCTGC(4/8)
BsrFI 2527 ACCGGT [GA]^CCGG[CT]
Cfr10I 2527 ACCGGT [GA]^CCGG[CT]
DraIII 983 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1922 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2535 GAATTC G^AATTC
EcoRV 3217 GATATC GAT^ATC
FspI 2631 TGCGCA TGC^GCA
HgaI 5038 GACGC GACGC(5/10)
KpnI 6111 GGTACC GGTAC^C
NciI 2289 CCGGG CC^[GC]GG
NdeI 2404 CATATG CA^TATG
NheI 1696 GCTAGC G^CTAGC
PflMI 6057 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2527 ACCGGT A^CCGGT
PmeI 5862 GTTTAAAC GTTT^AAAC
SbfI 4917 CCTGCAGG CCTGCA^GG
SnaBI 2748 TACGTA TAC^GTA
Sse8387I 4917 CCTGCAGG CCTGCA^GG
SspI 3385 AATATT AAT^ATT
SwaI 5643 ATTTAAAT ATTT^AAAT
TaqI 3568 TCGA T^CGA
TthHB8I 3568 TCGA T^CGA
Van91I 6057 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1564 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Alw44I 5189 GTGCAC G^TGCAC
6217 GTGCAC
AocI 346 CCTGAGG CC^T[ACGT]AGG
3811 CCTGAGG
Aor51HI 4561 AGCGCT AGC^GCT
4926 AGCGCT
ApaLI 5189 GTGCAC G^TGCAC
6217 GTGCAC
AvaI 1127 CCCGAG C^[CT]CG[GA]G
1344 CCCGAG
AvrII 1006 CCTAGG C^CTAGG
4645 CCTAGG
BalI 686 TGGCCA TGG^CCA
915 TGGCCA
BbrPI 5192 CACGTG CAC^GTG
5250 CACGTG
BlnI 1006 CCTAGG C^CTAGG
4645 CCTAGG
BsgI 2975 CTGCAC GTGCAG(16/14)
5310 CTGCAC
BsoBI 1127 CCCGAG C^[CT]CG[GA]G
1344 CCCGAG
Bsp1407I 793 TGTACA T^GTACA
4237 TGTACA
BspHI 2754 TCATGA T^CATGA
3854 TCATGA
BspLU11I 713 ACATGT A^CATGT
3645 ACATGT
BsrBI 4665 CCGCTC CCGCTC(-3/-3)
5167 GAGCGG
BsrDI 500 GCAATG GCAATG(2/0)
2186 GCAATG
BsrGI 793 TGTACA T^GTACA
4237 TGTACA
BstAPI 276 GCATGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3406 GCAAACAGTGC
BstEII 3718 GGTAACC G^GT[ACGT]ACC
4450 GGTGACC
BstPI 3718 GGTAACC G^GT[ACGT]ACC
4450 GGTGACC
Bsu36I 346 CCTGAGG CC^T[ACGT]AGG
3811 CCTGAGG
EaeI 686 TGGCCA [CT]^GGCC[GA]
915 TGGCCA
Eco47III 4561 AGCGCT AGC^GCT
4926 AGCGCT
Eco81I 346 CCTGAGG CC^T[ACGT]AGG
3811 CCTGAGG
EcoO65I 3718 GGTAACC G^GT[ACGT]ACC
4450 GGTGACC
MluNI 686 TGGCCA TGG^CCA
915 TGGCCA
MscI 686 TGGCCA TGG^CCA
915 TGGCCA
PmaCI 5192 CACGTG CAC^GTG
5250 CACGTG
PmlI 5192 CACGTG CAC^GTG
5250 CACGTG
PvuII 413 CAGCTG CAG^CTG
1445 CAGCTG
RcaI 2754 TCATGA T^CATGA
3854 TCATGA
SexAI 4403 ACCTGGT A^CC[AT]GGT
4892 ACCTGGT
SspBI 793 TGTACA T^GTACA
4237 TGTACA
StuI 1660 AGGCCT AGG^CCT
6158 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI AspHI AspI
AvaII BanI BanII BbeI BbsI
BbvCI BbvI BciVI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NarI NcoI NdeII NlaIII NlaIV
NspI PflFI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I XcmI XhoII