################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AccII AccIII AclI AflII AscI AseI AsnI BcgI BclI BfrI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BspDI BspEI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CelII ClaI CpoI DrdI EagI EclXI Eco52I EcoT22I FbaI FseI HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MunI MvnI NaeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI ScaI SfiI SfuI SgrAI SmaI SpeI SphI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 750 GACGTC GACGT^C Acc65I 6111 GGTACC G^GTACC AccI 5753 GTAGAC GT^[AC][GT]AC AgeI 2527 ACCGGT A^CCGGT AhdI 1922 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1781 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 4067 GGGCCC GGGCC^C Asp700I 1564 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 6111 GGTACC G^GTACC AspEI 1922 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2631 TGCGCA TGC^GCA BamHI 1726 GGATCC G^GATCC BcnI 2289 CCGGG CC^[GC]GG BglI 3808 GCCCCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4300 AGATCT A^GATCT BsaWI 2527 ACCGGT [AT]^CCGG[AT] BsmBI 1996 CGTCTC CGTCTC(1/5) BsmI 48 GAATGC GAATGC(1/-1) BspMI 2246 GCAGGT ACCTGC(4/8) BsrFI 2527 ACCGGT [GA]^CCGG[CT] Cfr10I 2527 ACCGGT [GA]^CCGG[CT] DraIII 983 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1922 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2535 GAATTC G^AATTC EcoRV 3217 GATATC GAT^ATC FspI 2631 TGCGCA TGC^GCA HgaI 5038 GACGC GACGC(5/10) KpnI 6111 GGTACC GGTAC^C NciI 2289 CCGGG CC^[GC]GG NdeI 2404 CATATG CA^TATG NheI 1696 GCTAGC G^CTAGC PflMI 6057 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2527 ACCGGT A^CCGGT PmeI 5862 GTTTAAAC GTTT^AAAC SbfI 4917 CCTGCAGG CCTGCA^GG SnaBI 2748 TACGTA TAC^GTA Sse8387I 4917 CCTGCAGG CCTGCA^GG SspI 3385 AATATT AAT^ATT SwaI 5643 ATTTAAAT ATTT^AAAT TaqI 3568 TCGA T^CGA TthHB8I 3568 TCGA T^CGA Van91I 6057 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1564 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### Alw44I 5189 GTGCAC G^TGCAC 6217 GTGCAC AocI 346 CCTGAGG CC^T[ACGT]AGG 3811 CCTGAGG Aor51HI 4561 AGCGCT AGC^GCT 4926 AGCGCT ApaLI 5189 GTGCAC G^TGCAC 6217 GTGCAC AvaI 1127 CCCGAG C^[CT]CG[GA]G 1344 CCCGAG AvrII 1006 CCTAGG C^CTAGG 4645 CCTAGG BalI 686 TGGCCA TGG^CCA 915 TGGCCA BbrPI 5192 CACGTG CAC^GTG 5250 CACGTG BlnI 1006 CCTAGG C^CTAGG 4645 CCTAGG BsgI 2975 CTGCAC GTGCAG(16/14) 5310 CTGCAC BsoBI 1127 CCCGAG C^[CT]CG[GA]G 1344 CCCGAG Bsp1407I 793 TGTACA T^GTACA 4237 TGTACA BspHI 2754 TCATGA T^CATGA 3854 TCATGA BspLU11I 713 ACATGT A^CATGT 3645 ACATGT BsrBI 4665 CCGCTC CCGCTC(-3/-3) 5167 GAGCGG BsrDI 500 GCAATG GCAATG(2/0) 2186 GCAATG BsrGI 793 TGTACA T^GTACA 4237 TGTACA BstAPI 276 GCATGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3406 GCAAACAGTGC BstEII 3718 GGTAACC G^GT[ACGT]ACC 4450 GGTGACC BstPI 3718 GGTAACC G^GT[ACGT]ACC 4450 GGTGACC Bsu36I 346 CCTGAGG CC^T[ACGT]AGG 3811 CCTGAGG EaeI 686 TGGCCA [CT]^GGCC[GA] 915 TGGCCA Eco47III 4561 AGCGCT AGC^GCT 4926 AGCGCT Eco81I 346 CCTGAGG CC^T[ACGT]AGG 3811 CCTGAGG EcoO65I 3718 GGTAACC G^GT[ACGT]ACC 4450 GGTGACC MluNI 686 TGGCCA TGG^CCA 915 TGGCCA MscI 686 TGGCCA TGG^CCA 915 TGGCCA PmaCI 5192 CACGTG CAC^GTG 5250 CACGTG PmlI 5192 CACGTG CAC^GTG 5250 CACGTG PvuII 413 CAGCTG CAG^CTG 1445 CAGCTG RcaI 2754 TCATGA T^CATGA 3854 TCATGA SexAI 4403 ACCTGGT A^CC[AT]GGT 4892 ACCTGGT SspBI 793 TGTACA T^GTACA 4237 TGTACA StuI 1660 AGGCCT AGG^CCT 6158 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI AspHI AspI AvaII BanI BanII BbeI BbsI BbvCI BbvI BciVI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NarI NcoI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I XcmI XhoII