##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AgeI Aor51HI AscI AseI
AsnI Asp718I AvrII BbeI BlnI
BsiWI BssHII Bst1107I BstBI BstZ17I
CpoI Eco47III EcoRI EcoT22I FseI
HpaI KasI KpnI KspI MfeI
MluI MunI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 4021 CGCG CG^CG
AccIII 375 TCCGGA T^CCGGA
AclI 4463 AACGTT AA^CGTT
AflII 1281 CTTAAG C^TTAAG
AhdI 1287 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4775 CCTGAGG CC^T[ACGT]AGG
ApaI 2696 GGGCCC GGGCC^C
AspEI 1287 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1188 TGCGCA TGC^GCA
BanI 2764 GGTGCC G^G[CT][GA]CC
BfrI 1281 CTTAAG C^TTAAG
BglI 853 GCCCAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2381 AGATCT A^GATCT
BsaBI 4794 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 375 TCCGGA [AT]^CCGG[AT]
BseAI 375 TCCGGA T^CCGGA
BsiEI 3860 CGGCCG CG[GA][CT]^CG
BspDI 5349 ATCGAT AT^CGAT
BspEI 375 TCCGGA T^CCGGA
BspHI 5166 TCATGA T^CATGA
BspMI 3313 GCAGGT ACCTGC(4/8)
BsrBI 2171 CCGCTC CCGCTC(-3/-3)
BssSI 4406 CACGAG CACGAG(-5/-1)
BstEII 933 GGTTACC G^GT[ACGT]ACC
BstPI 933 GGTTACC G^GT[ACGT]ACC
BstUI 4021 CGCG CG^CG
Bsu36I 4775 CCTGAGG CC^T[ACGT]AGG
ClaI 5349 ATCGAT AT^CGAT
DraI 4171 TTTAAA TTT^AAA
EagI 3860 CGGCCG C^GGCCG
Eam1105I 1287 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3860 CGGCCG C^GGCCG
Eco52I 3860 CGGCCG C^GGCCG
Eco81I 4775 CCTGAGG CC^T[ACGT]AGG
EcoO65I 933 GGTTACC G^GT[ACGT]ACC
EcoRV 1645 GATATC GAT^ATC
FauI 1108 GCGGG CCCGC(4/6)
FspI 1188 TGCGCA TGC^GCA
HgaI 1552 GACGC GACGC(5/10)
MamI 4794 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 375 TCCGGA T^CCGGA
MvnI 4021 CGCG CG^CG
NaeI 3858 GCCGGC GCC^GGC
NgoMIV 3858 GCCGGC G^CCGGC
PpuMI 2497 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 3735 GACAACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4463 AACGTT AA^CGTT
RcaI 5166 TCATGA T^CATGA
SmaI 3575 CCCGGG CCC^GGG
SspI 5477 AATATT AAT^ATT
StuI 2779 AGGCCT AGG^CCT
XcmI 4674 CCACTTTCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3575 CCCGGG C^CCGGG
XmaI 3575 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AatII 1287 GACGTC GACGT^C
1674 GACGTC
AflIII 1376 ACATGT A^C[GA][CT]GT
3126 ACATGT
Asp700I 3329 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5307 GAAATCATTC
AspI 1885 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2524 GACTTAGTC
AvaI 2842 CCCGAG C^[CT]CG[GA]G
3575 CCCGGG
BamHI 964 GGATCC G^GATCC
4254 GGATCC
BbrPI 1123 CACGTG CAC^GTG
5634 CACGTG
BbvCI 997 CCTCAGC CCTCAGC(-5/-2)
2271 CCTCAGC
BciVI 1767 GGATAC GTATCC(6/5)
3849 GTATCC
BclI 3003 TGATCA T^GATCA
4796 TGATCA
BsaAI 1123 CACGTG [CT]AC^GT[GA]
5634 CACGTG
BsmBI 2059 CGTCTC CGTCTC(1/5)
2656 GAGACG
BsmI 4077 GAATGC GAATGC(1/-1)
5684 GCATTC
BsoBI 2842 CCCGAG C^[CT]CG[GA]G
3575 CCCGGG
Bsp1407I 2354 TGTACA T^GTACA
2427 TGTACA
BspLU11I 1376 ACATGT A^CATGT
3126 ACATGT
BsrFI 52 GCCGGT [GA]^CCGG[CT]
3858 GCCGGC
BsrGI 2354 TGTACA T^GTACA
2427 TGTACA
Cfr10I 52 GCCGGT [GA]^CCGG[CT]
3858 GCCGGC
DraIII 5141 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
5634 CACGTGGTG
DrdI 3054 GACCAGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5537 GACCACTGTGTC
FbaI 3003 TGATCA T^GATCA
4796 TGATCA
HaeII 1596 GGCGCT [GA]GCGC^[CT]
4347 AGCGCC
HincII 1396 GTCAAC GT[CT]^[GA]AC
3655 GTTGAC
HindII 1396 GTCAAC GT[CT]^[GA]AC
3655 GTTGAC
NdeI 188 CATATG CA^TATG
1666 CATATG
PflFI 1885 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2524 GACTTAGTC
PmaCI 1123 CACGTG CAC^GTG
5634 CACGTG
PmlI 1123 CACGTG CAC^GTG
5634 CACGTG
SacI 665 GAGCTC GAGCT^C
1928 GAGCTC
SphI 1521 GCATGC GCATG^C
4269 GCATGC
SspBI 2354 TGTACA T^GTACA
2427 TGTACA
Tth111I 1885 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2524 GACTTAGTC
XbaI 474 TCTAGA T^CTAGA
5098 TCTAGA
XmnI 3329 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5307 GAAATCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaII BalI BanII
BbsI BbvI BcgI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII