################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AclI AgeI ApaI AscI Asp718I AviII AvrII BbeI BlnI BsaBI BsgI BsiWI BsmI BspDI BspHI BspLU11I BsrBI BssHII Bst1107I BstAPI BstBI BstZ17I ClaI CpoI EcoRI EcoRV FauI FseI FspI HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MunI NarI NdeI NotI NruI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 255 GACGTC GACGT^C AccII 55 CGCG CG^CG AccIII 3469 TCCGGA T^CCGGA AflII 2749 CTTAAG C^TTAAG AflIII 4061 ACACGT A^C[GA][CT]GT Alw44I 509 GTGCAC G^TGCAC Aor51HI 901 AGCGCT AGC^GCT ApaLI 509 GTGCAC G^TGCAC AseI 1608 ATTAAT AT^TAAT AsnI 1608 ATTAAT AT^TAAT Asp700I 1289 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 4107 CCCGAG C^[CT]CG[GA]G BamHI 883 GGATCC G^GATCC BanI 2188 GGTGCC G^G[CT][GA]CC BcgI 1330 GCATGCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1271 GTATCC GTATCC(6/5) BclI 3188 TGATCA T^GATCA BcnI 838 CCCGG CC^[GC]GG BfrI 2749 CTTAAG C^TTAAG BglII 3348 AGATCT A^GATCT BseAI 3469 TCCGGA T^CCGGA BsoBI 4107 CCCGAG C^[CT]CG[GA]G BspEI 3469 TCCGGA T^CCGGA BspMI 3797 ACCTGC ACCTGC(4/8) BsrDI 4190 CATTGC GCAATG(2/0) BsrFI 1878 GCCGGC [GA]^CCGG[CT] BstEII 719 GGTCACC G^GT[ACGT]ACC BstPI 719 GGTCACC G^GT[ACGT]ACC BstUI 55 CGCG CG^CG Cfr10I 1878 GCCGGC [GA]^CCGG[CT] DrdI 2690 GACAAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1876 CGGCCG C^GGCCG EclXI 1876 CGGCCG C^GGCCG Eco47III 901 AGCGCT AGC^GCT Eco52I 1876 CGGCCG C^GGCCG EcoNI 4114 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 719 GGTCACC G^GT[ACGT]ACC FbaI 3188 TGATCA T^GATCA MroI 3469 TCCGGA T^CCGGA MvnI 55 CGCG CG^CG NaeI 1878 GCCGGC GCC^GGC NciI 838 CCCGG CC^[GC]GG NgoMIV 1878 GCCGGC G^CCGGC NheI 2286 GCTAGC G^CTAGC PshAI 3129 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1608 ATTAAT AT^TAAT PvuII 1789 CAGCTG CAG^CTG SexAI 955 ACCAGGT A^CC[AT]GGT StuI 7 AGGCCT AGG^CCT TaqI 1319 TCGA T^CGA TthHB8I 1319 TCGA T^CGA XmnI 1289 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 752 GTCTAC GT^[AC][GT]AC 2038 GTCTAC AspI 2116 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4912 GACCATGTC BbrPI 495 CACGTG CAC^GTG 888 CACGTG BbvCI 2382 GCTGAGG CCTCAGC(-5/-2) 4399 GCTGAGG BsaI 3639 GAGACC GGTCTC(1/5) 4910 GAGACC BsaWI 33 TCCGGT [AT]^CCGG[AT] 3469 TCCGGA BsiEI 764 CGACCG CG[GA][CT]^CG 1876 CGGCCG BsmBI 257 CGTCTC CGTCTC(1/5) 670 GAGACG Bsp1407I 3414 TGTACA T^GTACA 4856 TGTACA BsrGI 3414 TGTACA T^GTACA 4856 TGTACA BssSI 729 CACGAG CACGAG(-5/-1) 1398 CTCGTG BstXI 859 CCAGCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4355 CCACAGATGTGG DraIII 495 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG 1778 CACCCAGTG EcoT22I 1585 ATGCAT ATGCA^T 2633 ATGCAT NsiI 1585 ATGCAT ATGCA^T 2633 ATGCAT PflFI 2116 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4912 GACCATGTC PmaCI 495 CACGTG CAC^GTG 888 CACGTG PmlI 495 CACGTG CAC^GTG 888 CACGTG PpuMI 4122 AGGACCT [GA]G^G[AT]CC[CT] 4685 GGGTCCC SacI 2026 GAGCTC GAGCT^C 4072 GAGCTC SapI 3898 GAAGAGC GCTCTTC(1/4) 1803 GCTCTTC ScaI 338 AGTACT AGT^ACT 819 AGTACT SpeI 3908 ACTAGT A^CTAGT 4545 ACTAGT SphI 1330 GCATGC GCATG^C 1583 GCATGC SspBI 3414 TGTACA T^GTACA 4856 TGTACA Tth111I 2116 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4912 GACCATGTC XbaI 3307 TCTAGA T^CTAGA 4836 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AciI AcsI AcyI AfaI AhdI AluI AlwI AlwNI AocI ApoI AspEI AspHI AvaII BalI BanII BbsI BbvI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI Eam1105I EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII