##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

Acc65I      AclI        AgeI        ApaI        AscI        
Asp718I     AviII       AvrII       BbeI        BlnI        
BsaBI       BsgI        BsiWI       BsmI        BspDI       
BspHI       BspLU11I    BsrBI       BssHII      Bst1107I    
BstAPI      BstBI       BstZ17I     ClaI        CpoI        
EcoRI       EcoRV       FauI        FseI        FspI        
HincII      HindII      HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
NarI        NdeI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmeI        Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       Sse8387I    SwaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII         255  GACGTC               GACGT^C
AccII          55  CGCG                 CG^CG
AccIII       3469  TCCGGA               T^CCGGA
AflII        2749  CTTAAG               C^TTAAG
AflIII       4061  ACACGT               A^C[GA][CT]GT
Alw44I        509  GTGCAC               G^TGCAC
Aor51HI       901  AGCGCT               AGC^GCT
ApaLI         509  GTGCAC               G^TGCAC
AseI         1608  ATTAAT               AT^TAAT
AsnI         1608  ATTAAT               AT^TAAT
Asp700I      1289  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         4107  CCCGAG               C^[CT]CG[GA]G
BamHI         883  GGATCC               G^GATCC
BanI         2188  GGTGCC               G^G[CT][GA]CC
BcgI         1330  GCATGCACCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1271  GTATCC               GTATCC(6/5)
BclI         3188  TGATCA               T^GATCA
BcnI          838  CCCGG                CC^[GC]GG
BfrI         2749  CTTAAG               C^TTAAG
BglII        3348  AGATCT               A^GATCT
BseAI        3469  TCCGGA               T^CCGGA
BsoBI        4107  CCCGAG               C^[CT]CG[GA]G
BspEI        3469  TCCGGA               T^CCGGA
BspMI        3797  ACCTGC               ACCTGC(4/8)
BsrDI        4190  CATTGC               GCAATG(2/0)
BsrFI        1878  GCCGGC               [GA]^CCGG[CT]
BstEII        719  GGTCACC              G^GT[ACGT]ACC
BstPI         719  GGTCACC              G^GT[ACGT]ACC
BstUI          55  CGCG                 CG^CG
Cfr10I       1878  GCCGGC               [GA]^CCGG[CT]
DrdI         2690  GACAAGGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1876  CGGCCG               C^GGCCG
EclXI        1876  CGGCCG               C^GGCCG
Eco47III      901  AGCGCT               AGC^GCT
Eco52I       1876  CGGCCG               C^GGCCG
EcoNI        4114  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       719  GGTCACC              G^GT[ACGT]ACC
FbaI         3188  TGATCA               T^GATCA
MroI         3469  TCCGGA               T^CCGGA
MvnI           55  CGCG                 CG^CG
NaeI         1878  GCCGGC               GCC^GGC
NciI          838  CCCGG                CC^[GC]GG
NgoMIV       1878  GCCGGC               G^CCGGC
NheI         2286  GCTAGC               G^CTAGC
PshAI        3129  GACCTTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1608  ATTAAT               AT^TAAT
PvuII        1789  CAGCTG               CAG^CTG
SexAI         955  ACCAGGT              A^CC[AT]GGT
StuI            7  AGGCCT               AGG^CCT
TaqI         1319  TCGA                 T^CGA
TthHB8I      1319  TCGA                 T^CGA
XmnI         1289  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI          752  GTCTAC               GT^[AC][GT]AC
             2038  GTCTAC              
AspI         2116  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4912  GACCATGTC           
BbrPI         495  CACGTG               CAC^GTG
              888  CACGTG              
BbvCI        2382  GCTGAGG              CCTCAGC(-5/-2)
             4399  GCTGAGG             
BsaI         3639  GAGACC               GGTCTC(1/5)
             4910  GAGACC              
BsaWI          33  TCCGGT               [AT]^CCGG[AT]
             3469  TCCGGA              
BsiEI         764  CGACCG               CG[GA][CT]^CG
             1876  CGGCCG              
BsmBI         257  CGTCTC               CGTCTC(1/5)
              670  GAGACG              
Bsp1407I     3414  TGTACA               T^GTACA
             4856  TGTACA              
BsrGI        3414  TGTACA               T^GTACA
             4856  TGTACA              
BssSI         729  CACGAG               CACGAG(-5/-1)
             1398  CTCGTG              
BstXI         859  CCAGCATCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4355  CCACAGATGTGG        
DraIII        495  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1778  CACCCAGTG           
EcoT22I      1585  ATGCAT               ATGCA^T
             2633  ATGCAT              
NsiI         1585  ATGCAT               ATGCA^T
             2633  ATGCAT              
PflFI        2116  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4912  GACCATGTC           
PmaCI         495  CACGTG               CAC^GTG
              888  CACGTG              
PmlI          495  CACGTG               CAC^GTG
              888  CACGTG              
PpuMI        4122  AGGACCT              [GA]G^G[AT]CC[CT]
             4685  GGGTCCC             
SacI         2026  GAGCTC               GAGCT^C
             4072  GAGCTC              
SapI         3898  GAAGAGC              GCTCTTC(1/4)
             1803  GCTCTTC             
ScaI          338  AGTACT               AGT^ACT
              819  AGTACT              
SpeI         3908  ACTAGT               A^CTAGT
             4545  ACTAGT              
SphI         1330  GCATGC               GCATG^C
             1583  GCATGC              
SspBI        3414  TGTACA               T^GTACA
             4856  TGTACA              
Tth111I      2116  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4912  GACCATGTC           
XbaI         3307  TCTAGA               T^CTAGA
             4836  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AhdI        
AluI        AlwI        AlwNI       AocI        ApoI        
AspEI       AspHI       AvaII       BalI        BanII       
BbsI        BbvI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
Eam1105I    EarI        Eco81I      EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Van91I      
XcmI        XhoII