##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AclI AgeI ApaI AscI
Asp718I AviII AvrII BbeI BlnI
BsaBI BsgI BsiWI BsmI BspDI
BspHI BspLU11I BsrBI BssHII Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
EcoRI EcoRV FauI FseI FspI
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NarI NdeI NotI NruI NspV
PacI PaeR7I PinAI PmeI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 255 GACGTC GACGT^C
AccII 55 CGCG CG^CG
AccIII 3469 TCCGGA T^CCGGA
AflII 2749 CTTAAG C^TTAAG
AflIII 4061 ACACGT A^C[GA][CT]GT
Alw44I 509 GTGCAC G^TGCAC
Aor51HI 901 AGCGCT AGC^GCT
ApaLI 509 GTGCAC G^TGCAC
AseI 1608 ATTAAT AT^TAAT
AsnI 1608 ATTAAT AT^TAAT
Asp700I 1289 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 4107 CCCGAG C^[CT]CG[GA]G
BamHI 883 GGATCC G^GATCC
BanI 2188 GGTGCC G^G[CT][GA]CC
BcgI 1330 GCATGCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1271 GTATCC GTATCC(6/5)
BclI 3188 TGATCA T^GATCA
BcnI 838 CCCGG CC^[GC]GG
BfrI 2749 CTTAAG C^TTAAG
BglII 3348 AGATCT A^GATCT
BseAI 3469 TCCGGA T^CCGGA
BsoBI 4107 CCCGAG C^[CT]CG[GA]G
BspEI 3469 TCCGGA T^CCGGA
BspMI 3797 ACCTGC ACCTGC(4/8)
BsrDI 4190 CATTGC GCAATG(2/0)
BsrFI 1878 GCCGGC [GA]^CCGG[CT]
BstEII 719 GGTCACC G^GT[ACGT]ACC
BstPI 719 GGTCACC G^GT[ACGT]ACC
BstUI 55 CGCG CG^CG
Cfr10I 1878 GCCGGC [GA]^CCGG[CT]
DrdI 2690 GACAAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1876 CGGCCG C^GGCCG
EclXI 1876 CGGCCG C^GGCCG
Eco47III 901 AGCGCT AGC^GCT
Eco52I 1876 CGGCCG C^GGCCG
EcoNI 4114 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 719 GGTCACC G^GT[ACGT]ACC
FbaI 3188 TGATCA T^GATCA
MroI 3469 TCCGGA T^CCGGA
MvnI 55 CGCG CG^CG
NaeI 1878 GCCGGC GCC^GGC
NciI 838 CCCGG CC^[GC]GG
NgoMIV 1878 GCCGGC G^CCGGC
NheI 2286 GCTAGC G^CTAGC
PshAI 3129 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1608 ATTAAT AT^TAAT
PvuII 1789 CAGCTG CAG^CTG
SexAI 955 ACCAGGT A^CC[AT]GGT
StuI 7 AGGCCT AGG^CCT
TaqI 1319 TCGA T^CGA
TthHB8I 1319 TCGA T^CGA
XmnI 1289 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 752 GTCTAC GT^[AC][GT]AC
2038 GTCTAC
AspI 2116 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4912 GACCATGTC
BbrPI 495 CACGTG CAC^GTG
888 CACGTG
BbvCI 2382 GCTGAGG CCTCAGC(-5/-2)
4399 GCTGAGG
BsaI 3639 GAGACC GGTCTC(1/5)
4910 GAGACC
BsaWI 33 TCCGGT [AT]^CCGG[AT]
3469 TCCGGA
BsiEI 764 CGACCG CG[GA][CT]^CG
1876 CGGCCG
BsmBI 257 CGTCTC CGTCTC(1/5)
670 GAGACG
Bsp1407I 3414 TGTACA T^GTACA
4856 TGTACA
BsrGI 3414 TGTACA T^GTACA
4856 TGTACA
BssSI 729 CACGAG CACGAG(-5/-1)
1398 CTCGTG
BstXI 859 CCAGCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4355 CCACAGATGTGG
DraIII 495 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1778 CACCCAGTG
EcoT22I 1585 ATGCAT ATGCA^T
2633 ATGCAT
NsiI 1585 ATGCAT ATGCA^T
2633 ATGCAT
PflFI 2116 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4912 GACCATGTC
PmaCI 495 CACGTG CAC^GTG
888 CACGTG
PmlI 495 CACGTG CAC^GTG
888 CACGTG
PpuMI 4122 AGGACCT [GA]G^G[AT]CC[CT]
4685 GGGTCCC
SacI 2026 GAGCTC GAGCT^C
4072 GAGCTC
SapI 3898 GAAGAGC GCTCTTC(1/4)
1803 GCTCTTC
ScaI 338 AGTACT AGT^ACT
819 AGTACT
SpeI 3908 ACTAGT A^CTAGT
4545 ACTAGT
SphI 1330 GCATGC GCATG^C
1583 GCATGC
SspBI 3414 TGTACA T^GTACA
4856 TGTACA
Tth111I 2116 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4912 GACCATGTC
XbaI 3307 TCTAGA T^CTAGA
4836 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AcyI AfaI AhdI
AluI AlwI AlwNI AocI ApoI
AspEI AspHI AvaII BalI BanII
BbsI BbvI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eam1105I EarI Eco81I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII