##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccIII AhdI AocI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII BamHI BbeI BglI BlpI
Bpu1102I BsaBI BseAI BsiWI BspDI
BspEI BsrDI BssHII Bst1107I BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraIII DrdI Eam1105I Eco81I
EcoO65I EcoRI EcoT22I FseI FspI
HpaI KasI KpnI KspI MamI
MluI MroI NaeI NarI NgoMIV
NotI NruI NsiI PacI PaeR7I
PmeI PpuMI PshAI PshBI PvuI
RsrII SacII SbfI ScaI SfiI
SfoI SnaBI Sse8387I SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 116 GACGTC GACGT^C
AccI 2146 GTCGAC GT^[AC][GT]AC
AclI 4591 AACGTT AA^CGTT
AgeI 1135 ACCGGT A^CCGGT
Alw44I 4387 GTGCAC G^TGCAC
Aor51HI 386 AGCGCT AGC^GCT
ApaI 2318 GGGCCC GGGCC^C
ApaLI 4387 GTGCAC G^TGCAC
AspI 3467 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3151 CCTAGG C^CTAGG
BclI 792 TGATCA T^GATCA
BglII 2250 AGATCT A^GATCT
BlnI 3151 CCTAGG C^CTAGG
BsaWI 1135 ACCGGT [AT]^CCGG[AT]
BsiEI 1976 CGGCCG CG[GA][CT]^CG
BsmBI 284 GAGACG CGTCTC(1/5)
Bsp1407I 4770 TGTACA T^GTACA
BspHI 1925 TCATGA T^CATGA
BspLU11I 4466 ACATGT A^CATGT
BsrGI 4770 TGTACA T^GTACA
BstBI 3256 TTCGAA TT^CGAA
EagI 1976 CGGCCG C^GGCCG
EclXI 1976 CGGCCG C^GGCCG
Eco47III 386 AGCGCT AGC^GCT
Eco52I 1976 CGGCCG C^GGCCG
EcoRV 1396 GATATC GAT^ATC
FauI 1178 CCCGC CCCGC(4/6)
FbaI 792 TGATCA T^GATCA
HincII 2146 GTCGAC GT[CT]^[GA]AC
HindII 2146 GTCGAC GT[CT]^[GA]AC
HindIII 3234 AAGCTT A^AGCTT
MfeI 1160 CAATTG C^AATTG
MspA1I 4177 CAGCTG C[AC]G^C[GT]G
MunI 1160 CAATTG C^AATTG
NdeI 392 CATATG CA^TATG
NspV 3256 TTCGAA TT^CGAA
PflFI 3467 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1135 ACCGGT A^CCGGT
Psp1406I 4591 AACGTT AA^CGTT
PvuII 4177 CAGCTG CAG^CTG
RcaI 1925 TCATGA T^CATGA
SalI 2146 GTCGAC G^TCGAC
SfuI 3256 TTCGAA TT^CGAA
SgrAI 1134 CACCGGTG C[GA]^CCGG[CT]G
SmaI 3037 CCCGGG CCC^GGG
SpeI 133 ACTAGT A^CTAGT
SspBI 4770 TGTACA T^GTACA
SspI 4213 AATATT AAT^ATT
Tth111I 3467 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3474 TCTAGA T^CTAGA
XmaCI 3037 CCCGGG C^CCGGG
XmaI 3037 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 49 CGCG CG^CG
73 CGCG
AcyI 116 GACGTC G[GA]^CG[CT]C
286 GACGCC
AflII 706 CTTAAG C^TTAAG
3109 CTTAAG
AflIII 2577 ACGTGT A^C[GA][CT]GT
4466 ACATGT
AlwNI 260 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
3313 CAGAGGCTG
BalI 3049 TGGCCA TGG^CCA
3877 TGGCCA
BbvCI 3183 CCTCAGC CCTCAGC(-5/-2)
4099 GCTGAGG
BcgI 1980 CGAGGAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
165 GCAGTCAGATCG
BciVI 14 GTATCC GTATCC(6/5)
1557 GTATCC
BfrI 706 CTTAAG C^TTAAG
3109 CTTAAG
BsaHI 116 GACGTC G[GA]^CG[CT]C
286 GACGCC
BsaI 631 GAGACC GGTCTC(1/5)
3465 GAGACC
BsmI 413 GCATTC GAATGC(1/-1)
3935 GCATTC
BsrFI 1135 ACCGGT [GA]^CCGG[CT]
4372 ACCGGC
BssSI 2894 CACGAG CACGAG(-5/-1)
637 CTCGTG
BstUI 49 CGCG CG^CG
73 CGCG
Cfr10I 1135 ACCGGT [GA]^CCGG[CT]
4372 ACCGGC
DraI 3096 TTTAAA TTT^AAA
4545 TTTAAA
DraII 2317 AGGGCCC [GA]G^G[ACGT]CC[CT]
3922 AGGCCCT
EcoO109I 2317 AGGGCCC [GA]G^G[ACGT]CC[CT]
3922 AGGCCCT
HaeII 386 AGCGCT [GA]GCGC^[CT]
4375 GGCGCT
HgaI 268 GACGC GACGC(5/10)
286 GACGC
Hin1I 116 GACGTC G[GA]^CG[CT]C
286 GACGCC
MluNI 3049 TGGCCA TGG^CCA
3877 TGGCCA
MscI 3049 TGGCCA TGG^CCA
3877 TGGCCA
MvnI 49 CGCG CG^CG
73 CGCG
NcoI 1230 CCATGG C^CATGG
2609 CCATGG
NheI 1998 GCTAGC G^CTAGC
4005 GCTAGC
SacI 1847 GAGCTC GAGCT^C
2637 GAGCTC
SexAI 1664 ACCTGGT A^CC[AT]GGT
4408 ACCTGGT
SphI 1250 GCATGC GCATG^C
4112 GCATGC
StuI 2541 AGGCCT AGG^CCT
4294 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanI
BanII BbrPI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII