##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

Acc65I      AccIII      AhdI        AocI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       BamHI       BbeI        BglI        BlpI        
Bpu1102I    BsaBI       BseAI       BsiWI       BspDI       
BspEI       BsrDI       BssHII      Bst1107I    BstEII      
BstPI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraIII      DrdI        Eam1105I    Eco81I      
EcoO65I     EcoRI       EcoT22I     FseI        FspI        
HpaI        KasI        KpnI        KspI        MamI        
MluI        MroI        NaeI        NarI        NgoMIV      
NotI        NruI        NsiI        PacI        PaeR7I      
PmeI        PpuMI       PshAI       PshBI       PvuI        
RsrII       SacII       SbfI        ScaI        SfiI        
SfoI        SnaBI       Sse8387I    SwaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII         116  GACGTC               GACGT^C
AccI         2146  GTCGAC               GT^[AC][GT]AC
AclI         4591  AACGTT               AA^CGTT
AgeI         1135  ACCGGT               A^CCGGT
Alw44I       4387  GTGCAC               G^TGCAC
Aor51HI       386  AGCGCT               AGC^GCT
ApaI         2318  GGGCCC               GGGCC^C
ApaLI        4387  GTGCAC               G^TGCAC
AspI         3467  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        3151  CCTAGG               C^CTAGG
BclI          792  TGATCA               T^GATCA
BglII        2250  AGATCT               A^GATCT
BlnI         3151  CCTAGG               C^CTAGG
BsaWI        1135  ACCGGT               [AT]^CCGG[AT]
BsiEI        1976  CGGCCG               CG[GA][CT]^CG
BsmBI         284  GAGACG               CGTCTC(1/5)
Bsp1407I     4770  TGTACA               T^GTACA
BspHI        1925  TCATGA               T^CATGA
BspLU11I     4466  ACATGT               A^CATGT
BsrGI        4770  TGTACA               T^GTACA
BstBI        3256  TTCGAA               TT^CGAA
EagI         1976  CGGCCG               C^GGCCG
EclXI        1976  CGGCCG               C^GGCCG
Eco47III      386  AGCGCT               AGC^GCT
Eco52I       1976  CGGCCG               C^GGCCG
EcoRV        1396  GATATC               GAT^ATC
FauI         1178  CCCGC                CCCGC(4/6)
FbaI          792  TGATCA               T^GATCA
HincII       2146  GTCGAC               GT[CT]^[GA]AC
HindII       2146  GTCGAC               GT[CT]^[GA]AC
HindIII      3234  AAGCTT               A^AGCTT
MfeI         1160  CAATTG               C^AATTG
MspA1I       4177  CAGCTG               C[AC]G^C[GT]G
MunI         1160  CAATTG               C^AATTG
NdeI          392  CATATG               CA^TATG
NspV         3256  TTCGAA               TT^CGAA
PflFI        3467  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1135  ACCGGT               A^CCGGT
Psp1406I     4591  AACGTT               AA^CGTT
PvuII        4177  CAGCTG               CAG^CTG
RcaI         1925  TCATGA               T^CATGA
SalI         2146  GTCGAC               G^TCGAC
SfuI         3256  TTCGAA               TT^CGAA
SgrAI        1134  CACCGGTG             C[GA]^CCGG[CT]G
SmaI         3037  CCCGGG               CCC^GGG
SpeI          133  ACTAGT               A^CTAGT
SspBI        4770  TGTACA               T^GTACA
SspI         4213  AATATT               AAT^ATT
Tth111I      3467  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3474  TCTAGA               T^CTAGA
XmaCI        3037  CCCGGG               C^CCGGG
XmaI         3037  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII          49  CGCG                 CG^CG
               73  CGCG                
AcyI          116  GACGTC               G[GA]^CG[CT]C
              286  GACGCC              
AflII         706  CTTAAG               C^TTAAG
             3109  CTTAAG              
AflIII       2577  ACGTGT               A^C[GA][CT]GT
             4466  ACATGT              
AlwNI         260  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3313  CAGAGGCTG           
BalI         3049  TGGCCA               TGG^CCA
             3877  TGGCCA              
BbvCI        3183  CCTCAGC              CCTCAGC(-5/-2)
             4099  GCTGAGG             
BcgI         1980  CGAGGAAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              165  GCAGTCAGATCG        
BciVI          14  GTATCC               GTATCC(6/5)
             1557  GTATCC              
BfrI          706  CTTAAG               C^TTAAG
             3109  CTTAAG              
BsaHI         116  GACGTC               G[GA]^CG[CT]C
              286  GACGCC              
BsaI          631  GAGACC               GGTCTC(1/5)
             3465  GAGACC              
BsmI          413  GCATTC               GAATGC(1/-1)
             3935  GCATTC              
BsrFI        1135  ACCGGT               [GA]^CCGG[CT]
             4372  ACCGGC              
BssSI        2894  CACGAG               CACGAG(-5/-1)
              637  CTCGTG              
BstUI          49  CGCG                 CG^CG
               73  CGCG                
Cfr10I       1135  ACCGGT               [GA]^CCGG[CT]
             4372  ACCGGC              
DraI         3096  TTTAAA               TTT^AAA
             4545  TTTAAA              
DraII        2317  AGGGCCC              [GA]G^G[ACGT]CC[CT]
             3922  AGGCCCT             
EcoO109I     2317  AGGGCCC              [GA]G^G[ACGT]CC[CT]
             3922  AGGCCCT             
HaeII         386  AGCGCT               [GA]GCGC^[CT]
             4375  GGCGCT              
HgaI          268  GACGC                GACGC(5/10)
              286  GACGC               
Hin1I         116  GACGTC               G[GA]^CG[CT]C
              286  GACGCC              
MluNI        3049  TGGCCA               TGG^CCA
             3877  TGGCCA              
MscI         3049  TGGCCA               TGG^CCA
             3877  TGGCCA              
MvnI           49  CGCG                 CG^CG
               73  CGCG                
NcoI         1230  CCATGG               C^CATGG
             2609  CCATGG              
NheI         1998  GCTAGC               G^CTAGC
             4005  GCTAGC              
SacI         1847  GAGCTC               GAGCT^C
             2637  GAGCTC              
SexAI        1664  ACCTGGT              A^CC[AT]GGT
             4408  ACCTGGT             
SphI         1250  GCATGC               GCATG^C
             4112  GCATGC              
StuI         2541  AGGCCT               AGG^CCT
             4294  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaI        AvaII       BanI        
BanII       BbrPI       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        EcoNI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PmaCI       PmlI        PstI        RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII