################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## Acc65I AccIII AhdI AocI AscI AseI AsnI Asp700I Asp718I AspEI AviII BamHI BbeI BglI BlpI Bpu1102I BsaBI BseAI BsiWI BspDI BspEI BsrDI BssHII Bst1107I BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI Eam1105I Eco81I EcoO65I EcoRI EcoT22I FseI FspI HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NgoMIV NotI NruI NsiI PacI PaeR7I PmeI PpuMI PshAI PshBI PvuI RsrII SacII SbfI ScaI SfiI SfoI SnaBI Sse8387I SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 116 GACGTC GACGT^C AccI 2146 GTCGAC GT^[AC][GT]AC AclI 4591 AACGTT AA^CGTT AgeI 1135 ACCGGT A^CCGGT Alw44I 4387 GTGCAC G^TGCAC Aor51HI 386 AGCGCT AGC^GCT ApaI 2318 GGGCCC GGGCC^C ApaLI 4387 GTGCAC G^TGCAC AspI 3467 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3151 CCTAGG C^CTAGG BclI 792 TGATCA T^GATCA BglII 2250 AGATCT A^GATCT BlnI 3151 CCTAGG C^CTAGG BsaWI 1135 ACCGGT [AT]^CCGG[AT] BsiEI 1976 CGGCCG CG[GA][CT]^CG BsmBI 284 GAGACG CGTCTC(1/5) Bsp1407I 4770 TGTACA T^GTACA BspHI 1925 TCATGA T^CATGA BspLU11I 4466 ACATGT A^CATGT BsrGI 4770 TGTACA T^GTACA BstBI 3256 TTCGAA TT^CGAA EagI 1976 CGGCCG C^GGCCG EclXI 1976 CGGCCG C^GGCCG Eco47III 386 AGCGCT AGC^GCT Eco52I 1976 CGGCCG C^GGCCG EcoRV 1396 GATATC GAT^ATC FauI 1178 CCCGC CCCGC(4/6) FbaI 792 TGATCA T^GATCA HincII 2146 GTCGAC GT[CT]^[GA]AC HindII 2146 GTCGAC GT[CT]^[GA]AC HindIII 3234 AAGCTT A^AGCTT MfeI 1160 CAATTG C^AATTG MspA1I 4177 CAGCTG C[AC]G^C[GT]G MunI 1160 CAATTG C^AATTG NdeI 392 CATATG CA^TATG NspV 3256 TTCGAA TT^CGAA PflFI 3467 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1135 ACCGGT A^CCGGT Psp1406I 4591 AACGTT AA^CGTT PvuII 4177 CAGCTG CAG^CTG RcaI 1925 TCATGA T^CATGA SalI 2146 GTCGAC G^TCGAC SfuI 3256 TTCGAA TT^CGAA SgrAI 1134 CACCGGTG C[GA]^CCGG[CT]G SmaI 3037 CCCGGG CCC^GGG SpeI 133 ACTAGT A^CTAGT SspBI 4770 TGTACA T^GTACA SspI 4213 AATATT AAT^ATT Tth111I 3467 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3474 TCTAGA T^CTAGA XmaCI 3037 CCCGGG C^CCGGG XmaI 3037 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 49 CGCG CG^CG 73 CGCG AcyI 116 GACGTC G[GA]^CG[CT]C 286 GACGCC AflII 706 CTTAAG C^TTAAG 3109 CTTAAG AflIII 2577 ACGTGT A^C[GA][CT]GT 4466 ACATGT AlwNI 260 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 3313 CAGAGGCTG BalI 3049 TGGCCA TGG^CCA 3877 TGGCCA BbvCI 3183 CCTCAGC CCTCAGC(-5/-2) 4099 GCTGAGG BcgI 1980 CGAGGAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 165 GCAGTCAGATCG BciVI 14 GTATCC GTATCC(6/5) 1557 GTATCC BfrI 706 CTTAAG C^TTAAG 3109 CTTAAG BsaHI 116 GACGTC G[GA]^CG[CT]C 286 GACGCC BsaI 631 GAGACC GGTCTC(1/5) 3465 GAGACC BsmI 413 GCATTC GAATGC(1/-1) 3935 GCATTC BsrFI 1135 ACCGGT [GA]^CCGG[CT] 4372 ACCGGC BssSI 2894 CACGAG CACGAG(-5/-1) 637 CTCGTG BstUI 49 CGCG CG^CG 73 CGCG Cfr10I 1135 ACCGGT [GA]^CCGG[CT] 4372 ACCGGC DraI 3096 TTTAAA TTT^AAA 4545 TTTAAA DraII 2317 AGGGCCC [GA]G^G[ACGT]CC[CT] 3922 AGGCCCT EcoO109I 2317 AGGGCCC [GA]G^G[ACGT]CC[CT] 3922 AGGCCCT HaeII 386 AGCGCT [GA]GCGC^[CT] 4375 GGCGCT HgaI 268 GACGC GACGC(5/10) 286 GACGC Hin1I 116 GACGTC G[GA]^CG[CT]C 286 GACGCC MluNI 3049 TGGCCA TGG^CCA 3877 TGGCCA MscI 3049 TGGCCA TGG^CCA 3877 TGGCCA MvnI 49 CGCG CG^CG 73 CGCG NcoI 1230 CCATGG C^CATGG 2609 CCATGG NheI 1998 GCTAGC G^CTAGC 4005 GCTAGC SacI 1847 GAGCTC GAGCT^C 2637 GAGCTC SexAI 1664 ACCTGGT A^CC[AT]GGT 4408 ACCTGGT SphI 1250 GCATGC GCATG^C 4112 GCATGC StuI 2541 AGGCCT AGG^CCT 4294 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanI BanII BbrPI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoNI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII