################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AccIII AclI AcsI Alw44I ApaLI ApoI AscI AseI AsnI AvrII BbrPI BlnI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI MfeI MluI MroI MunI NotI NruI NsiI PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 1425 GGTACC G^GTACC AflII 1973 CTTAAG C^TTAAG AflIII 2536 ACATGT A^C[GA][CT]GT AgeI 3247 ACCGGT A^CCGGT AhdI 3540 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 339 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1425 GGTACC G^GTACC AspEI 3540 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 848 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 4004 TGGCCA TGG^CCA BamHI 370 GGATCC G^GATCC BcgI 4262 GCAGTGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1161 TGATCA T^GATCA BfrI 1973 CTTAAG C^TTAAG BglI 945 GCCTGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4574 AGATCT A^GATCT BsaAI 4608 CACGTA [CT]AC^GT[GA] BsaBI 371 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 11 CGGTCG CG[GA][CT]^CG BspLU11I 2536 ACATGT A^CATGT BsrBI 2273 CCGCTC CCGCTC(-3/-3) BsrDI 1693 CATTGC GCAATG(2/0) BstAPI 2333 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 336 TTCGAA TT^CGAA Eam1105I 3540 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 1161 TGATCA T^GATCA KpnI 1425 GGTACC GGTAC^C KspI 3503 CCGCGG CCGC^GG MamI 371 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 4004 TGGCCA TGG^CCA MscI 4004 TGGCCA TGG^CCA NaeI 1648 GCCGGC GCC^GGC NcoI 462 CCATGG C^CATGG NgoMIV 1648 GCCGGC G^CCGGC NspV 336 TTCGAA TT^CGAA PflFI 848 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1278 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 3247 ACCGGT A^CCGGT SacII 3503 CCGCGG CCGC^GG SfuI 336 TTCGAA TT^CGAA StuI 2545 AGGCCT AGG^CCT Tth111I 848 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1278 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 339 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccI 2382 GTAGAC GT^[AC][GT]AC 4586 GTAGAC ApaI 466 GGGCCC GGGCC^C 3858 GGGCCC AviII 2659 TGCGCA TGC^GCA 3661 TGCGCA BbsI 1445 GAAGAC GAAGAC(2/6) 2769 GAAGAC BpuAI 1445 GAAGAC GAAGAC(2/6) 2769 GAAGAC BsaWI 3247 ACCGGT [AT]^CCGG[AT] 3268 TCCGGT BsrFI 1648 GCCGGC [GA]^CCGG[CT] 3247 ACCGGT BstYI 370 GGATCC [GA]^GATC[CT] 4574 AGATCT Cfr10I 1648 GCCGGC [GA]^CCGG[CT] 3247 ACCGGT DrdI 485 GACGAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4258 GACAGCAGTGTC EaeI 2824 TGGCCG [CT]^GGCC[GA] 4004 TGGCCA FspI 2659 TGCGCA TGC^GCA 3661 TGCGCA HincII 118 GTCAAC GT[CT]^[GA]AC 854 GTCAAC HindII 118 GTCAAC GT[CT]^[GA]AC 854 GTCAAC MflI 370 GGATCC [GA]^GATC[CT] 4574 AGATCT NdeI 880 CATATG CA^TATG 4086 CATATG PshAI 1312 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3422 GACCCAAGTC SexAI 474 ACCAGGT A^CC[AT]GGT 1146 ACCTGGT XhoII 370 GGATCC [GA]^GATC[CT] 4574 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI AspHI AvaI AvaII BanI BanII BbeI BbvCI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NheI NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XmaCI XmaI