##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AcsI Alw44I
ApaLI ApoI AscI AseI AsnI
AvrII BbrPI BlnI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI HindIII
HpaI MfeI MluI MroI MunI
NotI NruI NsiI PacI PaeR7I
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SfiI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1425 GGTACC G^GTACC
AflII 1973 CTTAAG C^TTAAG
AflIII 2536 ACATGT A^C[GA][CT]GT
AgeI 3247 ACCGGT A^CCGGT
AhdI 3540 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 339 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1425 GGTACC G^GTACC
AspEI 3540 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 848 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4004 TGGCCA TGG^CCA
BamHI 370 GGATCC G^GATCC
BcgI 4262 GCAGTGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1161 TGATCA T^GATCA
BfrI 1973 CTTAAG C^TTAAG
BglI 945 GCCTGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4574 AGATCT A^GATCT
BsaAI 4608 CACGTA [CT]AC^GT[GA]
BsaBI 371 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 11 CGGTCG CG[GA][CT]^CG
BspLU11I 2536 ACATGT A^CATGT
BsrBI 2273 CCGCTC CCGCTC(-3/-3)
BsrDI 1693 CATTGC GCAATG(2/0)
BstAPI 2333 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 336 TTCGAA TT^CGAA
Eam1105I 3540 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1161 TGATCA T^GATCA
KpnI 1425 GGTACC GGTAC^C
KspI 3503 CCGCGG CCGC^GG
MamI 371 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4004 TGGCCA TGG^CCA
MscI 4004 TGGCCA TGG^CCA
NaeI 1648 GCCGGC GCC^GGC
NcoI 462 CCATGG C^CATGG
NgoMIV 1648 GCCGGC G^CCGGC
NspV 336 TTCGAA TT^CGAA
PflFI 848 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1278 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3247 ACCGGT A^CCGGT
SacII 3503 CCGCGG CCGC^GG
SfuI 336 TTCGAA TT^CGAA
StuI 2545 AGGCCT AGG^CCT
Tth111I 848 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1278 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 339 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 2382 GTAGAC GT^[AC][GT]AC
4586 GTAGAC
ApaI 466 GGGCCC GGGCC^C
3858 GGGCCC
AviII 2659 TGCGCA TGC^GCA
3661 TGCGCA
BbsI 1445 GAAGAC GAAGAC(2/6)
2769 GAAGAC
BpuAI 1445 GAAGAC GAAGAC(2/6)
2769 GAAGAC
BsaWI 3247 ACCGGT [AT]^CCGG[AT]
3268 TCCGGT
BsrFI 1648 GCCGGC [GA]^CCGG[CT]
3247 ACCGGT
BstYI 370 GGATCC [GA]^GATC[CT]
4574 AGATCT
Cfr10I 1648 GCCGGC [GA]^CCGG[CT]
3247 ACCGGT
DrdI 485 GACGAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4258 GACAGCAGTGTC
EaeI 2824 TGGCCG [CT]^GGCC[GA]
4004 TGGCCA
FspI 2659 TGCGCA TGC^GCA
3661 TGCGCA
HincII 118 GTCAAC GT[CT]^[GA]AC
854 GTCAAC
HindII 118 GTCAAC GT[CT]^[GA]AC
854 GTCAAC
MflI 370 GGATCC [GA]^GATC[CT]
4574 AGATCT
NdeI 880 CATATG CA^TATG
4086 CATATG
PshAI 1312 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3422 GACCCAAGTC
SexAI 474 ACCAGGT A^CC[AT]GGT
1146 ACCTGGT
XhoII 370 GGATCC [GA]^GATC[CT]
4574 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI AspHI
AvaI AvaII BanI BanII BbeI
BbvCI BbvI BciVI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EarI Eco47III
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NheI NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XmaCI XmaI