##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
Alw44I AocI ApaLI AscI AseI
AsnI Asp718I AvrII BalI BbeI
BcgI BglII BlnI BseAI BsiWI
BspEI BspLU11I BssHII Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I CpoI
EagI EclXI Eco52I Eco81I EcoRV
FseI HpaI KasI KpnI KspI
MluI MluNI MroI MscI MvnI
NarI NcoI NotI NruI NspV
PacI PshAI PshBI Psp1406I PvuI
RsrII SacII SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I StuI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 43 GTCGAC GT^[AC][GT]AC
AflII 4945 CTTAAG C^TTAAG
AflIII 3457 ACACGT A^C[GA][CT]GT
AgeI 3719 ACCGGT A^CCGGT
ApaI 4675 GGGCCC GGGCC^C
AviII 3779 TGCGCA TGC^GCA
BamHI 2951 GGATCC G^GATCC
BanI 4180 GGTGCC G^G[CT][GA]CC
BbrPI 2481 CACGTG CAC^GTG
BciVI 2199 GGATAC GTATCC(6/5)
BfrI 4945 CTTAAG C^TTAAG
BsaAI 2481 CACGTG [CT]AC^GT[GA]
BsiEI 4102 CGACCG CG[GA][CT]^CG
BsmBI 4306 GAGACG CGTCTC(1/5)
BsmI 17 GAATGC GAATGC(1/-1)
BspDI 3472 ATCGAT AT^CGAT
BsrDI 5043 CATTGC GCAATG(2/0)
BssSI 3860 CACGAG CACGAG(-5/-1)
BstAPI 1245 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 3472 ATCGAT AT^CGAT
EcoT22I 4808 ATGCAT ATGCA^T
FspI 3779 TGCGCA TGC^GCA
MfeI 5539 CAATTG C^AATTG
MunI 5539 CAATTG C^AATTG
NheI 1168 GCTAGC G^CTAGC
NsiI 4808 ATGCAT ATGCA^T
PinAI 3719 ACCGGT A^CCGGT
PmaCI 2481 CACGTG CAC^GTG
PmeI 1664 GTTTAAAC GTTT^AAAC
PmlI 2481 CACGTG CAC^GTG
SalI 43 GTCGAC G^TCGAC
SmaI 3968 CCCGGG CCC^GGG
SpeI 2910 ACTAGT A^CTAGT
SphI 1542 GCATGC GCATG^C
SwaI 5285 ATTTAAAT ATTT^AAAT
XmaCI 3968 CCCGGG C^CCGGG
XmaI 3968 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 1915 GACGCC G[GA]^CG[CT]C
4282 GACGCC
Aor51HI 1153 AGCGCT AGC^GCT
1521 AGCGCT
BlpI 2508 GCTTAGC GC^T[ACGT]AGC
2923 GCTGAGC
Bpu1102I 2508 GCTTAGC GC^T[ACGT]AGC
2923 GCTGAGC
BsaBI 2826 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3604 GATGCCTATC
BsaHI 1915 GACGCC G[GA]^CG[CT]C
4282 GACGCC
BsaWI 3719 ACCGGT [AT]^CCGG[AT]
3935 TCCGGT
BspMI 4778 ACCTGC ACCTGC(4/8)
3264 GCAGGT
BsrBI 3987 CCGCTC CCGCTC(-3/-3)
4936 GAGCGG
BstEII 3090 GGTCACC G^GT[ACGT]ACC
4656 GGTAACC
BstPI 3090 GGTCACC G^GT[ACGT]ACC
4656 GGTAACC
CelII 2508 GCTTAGC GC^T[ACGT]AGC
2923 GCTGAGC
DraIII 2478 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
2526 CACGGTGTG
DrdI 1036 GACCCAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3352 GACTTTGATGTC
EaeI 3527 TGGCCG [CT]^GGCC[GA]
3613 CGGCCA
Eco47III 1153 AGCGCT AGC^GCT
1521 AGCGCT
EcoNI 1351 CCTACAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4427 CCTCCCCCAGG
EcoO65I 3090 GGTCACC G^GT[ACGT]ACC
4656 GGTAACC
Hin1I 1915 GACGCC G[GA]^CG[CT]C
4282 GACGCC
HindIII 1579 AAGCTT A^AGCTT
1946 AAGCTT
MamI 2826 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3604 GATGCCTATC
NdeI 1396 CATATG CA^TATG
2608 CATATG
NspI 960 ACATGC [GA]CATG^[CT]
1542 GCATGC
PaeR7I 595 CTCGAG C^TCGAG
3404 CTCGAG
SacI 2840 GAGCTC GAGCT^C
2926 GAGCTC
SspI 4627 AATATT AAT^ATT
4689 AATATT
XbaI 4302 TCTAGA T^CTAGA
5007 TCTAGA
XhoI 595 CTCGAG C^TCGAG
3404 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI AlwNI ApoI Asp700I AspEI
AspHI AspI AvaI AvaII BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspHI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI Eam1105I
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PflFI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmnI