################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII Acc65I AccII AccIII AclI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AvrII BalI BbeI BcgI BglII BlnI BseAI BsiWI BspEI BspLU11I BssHII Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CpoI EagI EclXI Eco52I Eco81I EcoRV FseI HpaI KasI KpnI KspI MluI MluNI MroI MscI MvnI NarI NcoI NotI NruI NspV PacI PshAI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I StuI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 43 GTCGAC GT^[AC][GT]AC AflII 4945 CTTAAG C^TTAAG AflIII 3457 ACACGT A^C[GA][CT]GT AgeI 3719 ACCGGT A^CCGGT ApaI 4675 GGGCCC GGGCC^C AviII 3779 TGCGCA TGC^GCA BamHI 2951 GGATCC G^GATCC BanI 4180 GGTGCC G^G[CT][GA]CC BbrPI 2481 CACGTG CAC^GTG BciVI 2199 GGATAC GTATCC(6/5) BfrI 4945 CTTAAG C^TTAAG BsaAI 2481 CACGTG [CT]AC^GT[GA] BsiEI 4102 CGACCG CG[GA][CT]^CG BsmBI 4306 GAGACG CGTCTC(1/5) BsmI 17 GAATGC GAATGC(1/-1) BspDI 3472 ATCGAT AT^CGAT BsrDI 5043 CATTGC GCAATG(2/0) BssSI 3860 CACGAG CACGAG(-5/-1) BstAPI 1245 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 3472 ATCGAT AT^CGAT EcoT22I 4808 ATGCAT ATGCA^T FspI 3779 TGCGCA TGC^GCA MfeI 5539 CAATTG C^AATTG MunI 5539 CAATTG C^AATTG NheI 1168 GCTAGC G^CTAGC NsiI 4808 ATGCAT ATGCA^T PinAI 3719 ACCGGT A^CCGGT PmaCI 2481 CACGTG CAC^GTG PmeI 1664 GTTTAAAC GTTT^AAAC PmlI 2481 CACGTG CAC^GTG SalI 43 GTCGAC G^TCGAC SmaI 3968 CCCGGG CCC^GGG SpeI 2910 ACTAGT A^CTAGT SphI 1542 GCATGC GCATG^C SwaI 5285 ATTTAAAT ATTT^AAAT XmaCI 3968 CCCGGG C^CCGGG XmaI 3968 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 1915 GACGCC G[GA]^CG[CT]C 4282 GACGCC Aor51HI 1153 AGCGCT AGC^GCT 1521 AGCGCT BlpI 2508 GCTTAGC GC^T[ACGT]AGC 2923 GCTGAGC Bpu1102I 2508 GCTTAGC GC^T[ACGT]AGC 2923 GCTGAGC BsaBI 2826 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3604 GATGCCTATC BsaHI 1915 GACGCC G[GA]^CG[CT]C 4282 GACGCC BsaWI 3719 ACCGGT [AT]^CCGG[AT] 3935 TCCGGT BspMI 4778 ACCTGC ACCTGC(4/8) 3264 GCAGGT BsrBI 3987 CCGCTC CCGCTC(-3/-3) 4936 GAGCGG BstEII 3090 GGTCACC G^GT[ACGT]ACC 4656 GGTAACC BstPI 3090 GGTCACC G^GT[ACGT]ACC 4656 GGTAACC CelII 2508 GCTTAGC GC^T[ACGT]AGC 2923 GCTGAGC DraIII 2478 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG 2526 CACGGTGTG DrdI 1036 GACCCAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3352 GACTTTGATGTC EaeI 3527 TGGCCG [CT]^GGCC[GA] 3613 CGGCCA Eco47III 1153 AGCGCT AGC^GCT 1521 AGCGCT EcoNI 1351 CCTACAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4427 CCTCCCCCAGG EcoO65I 3090 GGTCACC G^GT[ACGT]ACC 4656 GGTAACC Hin1I 1915 GACGCC G[GA]^CG[CT]C 4282 GACGCC HindIII 1579 AAGCTT A^AGCTT 1946 AAGCTT MamI 2826 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3604 GATGCCTATC NdeI 1396 CATATG CA^TATG 2608 CATATG NspI 960 ACATGC [GA]CATG^[CT] 1542 GCATGC PaeR7I 595 CTCGAG C^TCGAG 3404 CTCGAG SacI 2840 GAGCTC GAGCT^C 2926 GAGCTC SspI 4627 AATATT AAT^ATT 4689 AATATT XbaI 4302 TCTAGA T^CTAGA 5007 TCTAGA XhoI 595 CTCGAG C^TCGAG 3404 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AciI AcsI AfaI AhdI AluI AlwI AlwNI ApoI Asp700I AspEI AspHI AspI AvaI AvaII BanII BbsI BbvCI BbvI BclI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI Eam1105I EarI EcoO109I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflFI PflMI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmnI