################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BanI BbeI BbvCI BcgI BciVI BfrI BglI BlnI BsaBI BsaHI BsiWI BsrBI BsrFI BssHII Bst1107I BstBI BstZ17I Bsu36I CfoI Cfr10I CpoI EagI EclXI Eco47III Eco52I Eco81I FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PshBI Psp1406I PvuI RsrII SacII SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccII 1931 CGCG CG^CG AccIII 3034 TCCGGA T^CCGGA AflIII 4784 ACATGT A^C[GA][CT]GT AhdI 3324 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 3324 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 2682 CTCGGG C^[CT]CG[GA]G BbrPI 3024 CACGTG CAC^GTG BglII 1825 AGATCT A^GATCT BsaWI 3034 TCCGGA [AT]^CCGG[AT] BseAI 3034 TCCGGA T^CCGGA BsiEI 2486 CGACCG CG[GA][CT]^CG BsoBI 2682 CTCGGG C^[CT]CG[GA]G Bsp1407I 4340 TGTACA T^GTACA BspDI 1262 ATCGAT AT^CGAT BspEI 3034 TCCGGA T^CCGGA BspLU11I 4784 ACATGT A^CATGT BsrGI 4340 TGTACA T^GTACA BssSI 1248 CACGAG CACGAG(-5/-1) BstAPI 383 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1931 CGCG CG^CG ClaI 1262 ATCGAT AT^CGAT DraIII 4325 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 3324 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FauI 2144 GCGGG CCCGC(4/6) HgaI 1929 GACGC GACGC(5/10) MroI 3034 TCCGGA T^CCGGA MvnI 1931 CGCG CG^CG NdeI 4565 CATATG CA^TATG NheI 3269 GCTAGC G^CTAGC PmaCI 3024 CACGTG CAC^GTG PmeI 5190 GTTTAAAC GTTT^AAAC PmlI 3024 CACGTG CAC^GTG PshAI 4376 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 3710 GTCGAC G^TCGAC SapI 2975 GCTCTTC GCTCTTC(1/4) ScaI 5405 AGTACT AGT^ACT SmlI 2757 CTCAAG C^T[CT][GA]AG SspBI 4340 TGTACA T^GTACA XcmI 2517 CCAAACCCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 939 GTAGAC GT^[AC][GT]AC 3710 GTCGAC BamHI 1391 GGATCC G^GATCC 2434 GGATCC BsaAI 2256 TACGTG [CT]AC^GT[GA] 3024 CACGTG BsaI 4085 GAGACC GGTCTC(1/5) 4434 GGTCTC BstEII 1364 GGTTACC G^GT[ACGT]ACC 2825 GGTGACC BstPI 1364 GGTTACC G^GT[ACGT]ACC 2825 GGTGACC DrdI 684 GACCAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4426 GACCAACAGGTC EcoNI 1138 CCTTTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1226 CCTGGGAAAGG EcoO65I 1364 GGTTACC G^GT[ACGT]ACC 2825 GGTGACC EcoRI 2950 GAATTC G^AATTC 4144 GAATTC EcoRV 1560 GATATC GAT^ATC 4883 GATATC HindIII 1084 AAGCTT A^AGCTT 3817 AAGCTT MaeII 2257 ACGT A^CGT 3025 ACGT PstI 55 CTGCAG CTGCA^G 1614 CTGCAG SacI 1934 GAGCTC GAGCT^C 2031 GAGCTC SpeI 981 ACTAGT A^CTAGT 3140 ACTAGT SphI 644 GCATGC GCATG^C 1650 GCATGC XbaI 3457 TCTAGA T^CTAGA 5129 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BanII BbsI BbvI BclI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FbaI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI