##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BanI BbeI
BbvCI BcgI BciVI BfrI BglI
BlnI BsaBI BsaHI BsiWI BsrBI
BsrFI BssHII Bst1107I BstBI BstZ17I
Bsu36I CfoI Cfr10I CpoI EagI
EclXI Eco47III Eco52I Eco81I FseI
FspI HaeII HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PshBI
Psp1406I PvuI RsrII SacII SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 1931 CGCG CG^CG
AccIII 3034 TCCGGA T^CCGGA
AflIII 4784 ACATGT A^C[GA][CT]GT
AhdI 3324 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3324 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2682 CTCGGG C^[CT]CG[GA]G
BbrPI 3024 CACGTG CAC^GTG
BglII 1825 AGATCT A^GATCT
BsaWI 3034 TCCGGA [AT]^CCGG[AT]
BseAI 3034 TCCGGA T^CCGGA
BsiEI 2486 CGACCG CG[GA][CT]^CG
BsoBI 2682 CTCGGG C^[CT]CG[GA]G
Bsp1407I 4340 TGTACA T^GTACA
BspDI 1262 ATCGAT AT^CGAT
BspEI 3034 TCCGGA T^CCGGA
BspLU11I 4784 ACATGT A^CATGT
BsrGI 4340 TGTACA T^GTACA
BssSI 1248 CACGAG CACGAG(-5/-1)
BstAPI 383 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1931 CGCG CG^CG
ClaI 1262 ATCGAT AT^CGAT
DraIII 4325 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3324 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 2144 GCGGG CCCGC(4/6)
HgaI 1929 GACGC GACGC(5/10)
MroI 3034 TCCGGA T^CCGGA
MvnI 1931 CGCG CG^CG
NdeI 4565 CATATG CA^TATG
NheI 3269 GCTAGC G^CTAGC
PmaCI 3024 CACGTG CAC^GTG
PmeI 5190 GTTTAAAC GTTT^AAAC
PmlI 3024 CACGTG CAC^GTG
PshAI 4376 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 3710 GTCGAC G^TCGAC
SapI 2975 GCTCTTC GCTCTTC(1/4)
ScaI 5405 AGTACT AGT^ACT
SmlI 2757 CTCAAG C^T[CT][GA]AG
SspBI 4340 TGTACA T^GTACA
XcmI 2517 CCAAACCCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 939 GTAGAC GT^[AC][GT]AC
3710 GTCGAC
BamHI 1391 GGATCC G^GATCC
2434 GGATCC
BsaAI 2256 TACGTG [CT]AC^GT[GA]
3024 CACGTG
BsaI 4085 GAGACC GGTCTC(1/5)
4434 GGTCTC
BstEII 1364 GGTTACC G^GT[ACGT]ACC
2825 GGTGACC
BstPI 1364 GGTTACC G^GT[ACGT]ACC
2825 GGTGACC
DrdI 684 GACCAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4426 GACCAACAGGTC
EcoNI 1138 CCTTTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1226 CCTGGGAAAGG
EcoO65I 1364 GGTTACC G^GT[ACGT]ACC
2825 GGTGACC
EcoRI 2950 GAATTC G^AATTC
4144 GAATTC
EcoRV 1560 GATATC GAT^ATC
4883 GATATC
HindIII 1084 AAGCTT A^AGCTT
3817 AAGCTT
MaeII 2257 ACGT A^CGT
3025 ACGT
PstI 55 CTGCAG CTGCA^G
1614 CTGCAG
SacI 1934 GAGCTC GAGCT^C
2031 GAGCTC
SpeI 981 ACTAGT A^CTAGT
3140 ACTAGT
SphI 644 GCATGC GCATG^C
1650 GCATGC
XbaI 3457 TCTAGA T^CTAGA
5129 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BanII BbsI BbvI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
EcoT22I FbaI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI