################################## # NO CUT ENZYMES ( 45 enzymes) # ################################## AatII AgeI Alw44I ApaLI AscI AseI AsnI Asp700I AvrII BbrPI BlnI BsiWI BsmBI BspDI BssHII Bst1107I BstZ17I ClaI CpoI FseI MluI NdeI NheI NotI NruI PacI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SalI ScaI SfiI SgrAI SnaBI SpeI SphI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AclI 2198 AACGTT AA^CGTT AhdI 625 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 609 CCTAAGG CC^T[ACGT]AGG AspEI 625 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1709 TGCGCA TGC^GCA BbeI 16 GGCGCC GGCGC^C BbvCI 3229 CCTCAGC CCTCAGC(-5/-2) BsaAI 2866 TACGTG [CT]AC^GT[GA] BsaBI 5200 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 2532 CGGCCG CG[GA][CT]^CG BspHI 392 TCATGA T^CATGA Bsu36I 609 CCTAAGG CC^T[ACGT]AGG EagI 2532 CGGCCG C^GGCCG Eam1105I 625 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2532 CGGCCG C^GGCCG Eco52I 2532 CGGCCG C^GGCCG Eco81I 609 CCTAAGG CC^T[ACGT]AGG FspI 1709 TGCGCA TGC^GCA HpaI 4599 GTTAAC GTT^AAC KasI 16 GGCGCC G^GCGCC KspI 1316 CCGCGG CCGC^GG MamI 5200 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2306 CAATTG C^AATTG MunI 2306 CAATTG C^AATTG NarI 16 GGCGCC GG^CGCC PaeR7I 3172 CTCGAG C^TCGAG Psp1406I 2198 AACGTT AA^CGTT RcaI 392 TCATGA T^CATGA SacI 5110 GAGCTC GAGCT^C SacII 1316 CCGCGG CCGC^GG SexAI 3197 ACCTGGT A^CC[AT]GGT SfoI 16 GGCGCC GGC^GCC XcmI 2405 CCATTGTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3172 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 939 GGTACC G^GTACC 6174 GGTACC AccIII 3158 TCCGGA T^CCGGA 3323 TCCGGA AflII 1620 CTTAAG C^TTAAG 5314 CTTAAG Aor51HI 428 AGCGCT AGC^GCT 4685 AGCGCT ApaI 2752 GGGCCC GGGCC^C 3354 GGGCCC Asp718I 939 GGTACC G^GTACC 6174 GGTACC BamHI 2599 GGATCC G^GATCC 2692 GGATCC BciVI 3440 GTATCC GTATCC(6/5) 5585 GTATCC BfrI 1620 CTTAAG C^TTAAG 5314 CTTAAG BseAI 3158 TCCGGA T^CCGGA 3323 TCCGGA BsmI 174 GAATGC GAATGC(1/-1) 4698 GCATTC BspEI 3158 TCCGGA T^CCGGA 3323 TCCGGA BssSI 2917 CACGAG CACGAG(-5/-1) 4313 CTCGTG BstBI 1523 TTCGAA TT^CGAA 1565 TTCGAA BstXI 659 CCATGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4712 CCAAGTCAGTGG DraIII 687 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 4495 CACTGGGTG DrdI 259 GACTATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 850 GACGGAAATGTC Eco47III 428 AGCGCT AGC^GCT 4685 AGCGCT EcoRI 1897 GAATTC G^AATTC 5985 GAATTC EcoT22I 176 ATGCAT ATGCA^T 3379 ATGCAT HindIII 4831 AAGCTT A^AGCTT 5957 AAGCTT KpnI 939 GGTACC GGTAC^C 6174 GGTACC MroI 3158 TCCGGA T^CCGGA 3323 TCCGGA NsiI 176 ATGCAT ATGCA^T 3379 ATGCAT NspV 1523 TTCGAA TT^CGAA 1565 TTCGAA SapI 4071 GAAGAGC GCTCTTC(1/4) 1855 GCTCTTC SbfI 1473 CCTGCAGG CCTGCA^GG 3333 CCTGCAGG SfuI 1523 TTCGAA TT^CGAA 1565 TTCGAA Sse8387I 1473 CCTGCAGG CCTGCA^GG 3333 CCTGCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 150 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BclI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XhoII XmaCI XmaI