##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I AvrII BbrPI
BlnI BsiWI BsmBI BspDI BssHII
Bst1107I BstZ17I ClaI CpoI FseI
MluI NdeI NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SalI
ScaI SfiI SgrAI SnaBI SpeI
SphI SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AclI 2198 AACGTT AA^CGTT
AhdI 625 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 609 CCTAAGG CC^T[ACGT]AGG
AspEI 625 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1709 TGCGCA TGC^GCA
BbeI 16 GGCGCC GGCGC^C
BbvCI 3229 CCTCAGC CCTCAGC(-5/-2)
BsaAI 2866 TACGTG [CT]AC^GT[GA]
BsaBI 5200 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2532 CGGCCG CG[GA][CT]^CG
BspHI 392 TCATGA T^CATGA
Bsu36I 609 CCTAAGG CC^T[ACGT]AGG
EagI 2532 CGGCCG C^GGCCG
Eam1105I 625 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2532 CGGCCG C^GGCCG
Eco52I 2532 CGGCCG C^GGCCG
Eco81I 609 CCTAAGG CC^T[ACGT]AGG
FspI 1709 TGCGCA TGC^GCA
HpaI 4599 GTTAAC GTT^AAC
KasI 16 GGCGCC G^GCGCC
KspI 1316 CCGCGG CCGC^GG
MamI 5200 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2306 CAATTG C^AATTG
MunI 2306 CAATTG C^AATTG
NarI 16 GGCGCC GG^CGCC
PaeR7I 3172 CTCGAG C^TCGAG
Psp1406I 2198 AACGTT AA^CGTT
RcaI 392 TCATGA T^CATGA
SacI 5110 GAGCTC GAGCT^C
SacII 1316 CCGCGG CCGC^GG
SexAI 3197 ACCTGGT A^CC[AT]GGT
SfoI 16 GGCGCC GGC^GCC
XcmI 2405 CCATTGTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3172 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 939 GGTACC G^GTACC
6174 GGTACC
AccIII 3158 TCCGGA T^CCGGA
3323 TCCGGA
AflII 1620 CTTAAG C^TTAAG
5314 CTTAAG
Aor51HI 428 AGCGCT AGC^GCT
4685 AGCGCT
ApaI 2752 GGGCCC GGGCC^C
3354 GGGCCC
Asp718I 939 GGTACC G^GTACC
6174 GGTACC
BamHI 2599 GGATCC G^GATCC
2692 GGATCC
BciVI 3440 GTATCC GTATCC(6/5)
5585 GTATCC
BfrI 1620 CTTAAG C^TTAAG
5314 CTTAAG
BseAI 3158 TCCGGA T^CCGGA
3323 TCCGGA
BsmI 174 GAATGC GAATGC(1/-1)
4698 GCATTC
BspEI 3158 TCCGGA T^CCGGA
3323 TCCGGA
BssSI 2917 CACGAG CACGAG(-5/-1)
4313 CTCGTG
BstBI 1523 TTCGAA TT^CGAA
1565 TTCGAA
BstXI 659 CCATGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4712 CCAAGTCAGTGG
DraIII 687 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4495 CACTGGGTG
DrdI 259 GACTATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
850 GACGGAAATGTC
Eco47III 428 AGCGCT AGC^GCT
4685 AGCGCT
EcoRI 1897 GAATTC G^AATTC
5985 GAATTC
EcoT22I 176 ATGCAT ATGCA^T
3379 ATGCAT
HindIII 4831 AAGCTT A^AGCTT
5957 AAGCTT
KpnI 939 GGTACC GGTAC^C
6174 GGTACC
MroI 3158 TCCGGA T^CCGGA
3323 TCCGGA
NsiI 176 ATGCAT ATGCA^T
3379 ATGCAT
NspV 1523 TTCGAA TT^CGAA
1565 TTCGAA
SapI 4071 GAAGAGC GCTCTTC(1/4)
1855 GCTCTTC
SbfI 1473 CCTGCAGG CCTGCA^GG
3333 CCTGCAGG
SfuI 1523 TTCGAA TT^CGAA
1565 TTCGAA
Sse8387I 1473 CCTGCAGG CCTGCA^GG
3333 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcgI BclI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoRV EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XhoII XmaCI XmaI