################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AccIII AclI AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI AspEI AviII BamHI BbrPI BclI BglI BsaAI BsaBI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoRI FbaI FseI FspI HpaI KspI MamI MfeI MluI MroI MunI NdeI NotI NruI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI Sse8387I SspBI SwaI Van91I ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 1139 GACGTC GACGT^C Acc65I 4840 GGTACC G^GTACC AccI 3133 GTCTAC GT^[AC][GT]AC AflIII 4815 ACATGT A^C[GA][CT]GT Aor51HI 2927 AGCGCT AGC^GCT Asp718I 4840 GGTACC G^GTACC BalI 4579 TGGCCA TGG^CCA BbeI 104 GGCGCC GGCGC^C BcgI 4388 CGATGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2752 GTATCC GTATCC(6/5) BsaWI 164 ACCGGA [AT]^CCGG[AT] BspLU11I 4815 ACATGT A^CATGT BsrDI 1783 CATTGC GCAATG(2/0) BsrFI 78 GCCGGC [GA]^CCGG[CT] Cfr10I 78 GCCGGC [GA]^CCGG[CT] DraIII 582 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2927 AGCGCT AGC^GCT KasI 104 GGCGCC G^GCGCC KpnI 4840 GGTACC GGTAC^C MaeII 1140 ACGT A^CGT MluNI 4579 TGGCCA TGG^CCA MscI 4579 TGGCCA TGG^CCA NaeI 78 GCCGGC GCC^GGC NarI 104 GGCGCC GG^CGCC NcoI 616 CCATGG C^CATGG NgoMIV 78 GCCGGC G^CCGGC NheI 3048 GCTAGC G^CTAGC PaeR7I 912 CTCGAG C^TCGAG PshAI 1576 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 3917 CAGCTG CAG^CTG SacI 577 GAGCTC GAGCT^C SapI 749 GCTCTTC GCTCTTC(1/4) SexAI 286 ACCTGGT A^CC[AT]GGT SfoI 104 GGCGCC GGC^GCC SpeI 4885 ACTAGT A^CTAGT SspI 3842 AATATT AAT^ATT StuI 4363 AGGCCT AGG^CCT XbaI 1838 TCTAGA T^CTAGA XcmI 1808 CCACTCCTGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 912 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 149 CGCG CG^CG 155 CGCG Asp700I 397 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4476 GAAGAGGTTC AspI 285 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1136 GACGACGTC AvrII 1788 CCTAGG C^CTAGG 3381 CCTAGG BglII 4342 AGATCT A^GATCT 4450 AGATCT BlnI 1788 CCTAGG C^CTAGG 3381 CCTAGG BlpI 87 GCTCAGC GC^T[ACGT]AGC 4426 GCTGAGC Bpu1102I 87 GCTCAGC GC^T[ACGT]AGC 4426 GCTGAGC BsaI 3317 GAGACC GGTCTC(1/5) 2869 GGTCTC BsmI 4441 GAATGC GAATGC(1/-1) 4143 GCATTC BsrBI 85 CCGCTC CCGCTC(-3/-3) 21 GAGCGG BssSI 582 CACGAG CACGAG(-5/-1) 4741 CACGAG BstAPI 1251 GCATCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1778 GCATGCATTGC BstEII 782 GGTTACC G^GT[ACGT]ACC 2542 GGTAACC BstPI 782 GGTTACC G^GT[ACGT]ACC 2542 GGTAACC BstUI 149 CGCG CG^CG 155 CGCG CelII 87 GCTCAGC GC^T[ACGT]AGC 4426 GCTGAGC EaeI 47 CGGCCA [CT]^GGCC[GA] 4579 TGGCCA EcoO65I 782 GGTTACC G^GT[ACGT]ACC 2542 GGTAACC EcoRV 606 GATATC GAT^ATC 1179 GATATC HgaI 12 GCGTC GACGC(5/10) 3962 GCGTC HindIII 2181 AAGCTT A^AGCTT 3229 AAGCTT MspA1I 433 CCGCTG C[AC]G^C[GT]G 3917 CAGCTG MvnI 149 CGCG CG^CG 155 CGCG PflFI 285 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1136 GACGACGTC PstI 591 CTGCAG CTGCA^G 792 CTGCAG SmaI 129 CCCGGG CCC^GGG 717 CCCGGG SphI 1778 GCATGC GCATG^C 4004 GCATGC Tth111I 285 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1136 GACGACGTC XmaCI 129 CCCGGG C^CCGGG 717 CCCGGG XmaI 129 CCCGGG C^CCGGG 717 CCCGGG XmnI 397 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4476 GAAGAGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AciI AcsI AcyI AfaI AflII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BfrI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII