##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccIII AclI AgeI AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
AspEI AviII BamHI BbrPI BclI
BglI BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoRI FbaI
FseI FspI HpaI KspI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfuI SgrAI SnaBI
Sse8387I SspBI SwaI Van91I
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1139 GACGTC GACGT^C
Acc65I 4840 GGTACC G^GTACC
AccI 3133 GTCTAC GT^[AC][GT]AC
AflIII 4815 ACATGT A^C[GA][CT]GT
Aor51HI 2927 AGCGCT AGC^GCT
Asp718I 4840 GGTACC G^GTACC
BalI 4579 TGGCCA TGG^CCA
BbeI 104 GGCGCC GGCGC^C
BcgI 4388 CGATGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2752 GTATCC GTATCC(6/5)
BsaWI 164 ACCGGA [AT]^CCGG[AT]
BspLU11I 4815 ACATGT A^CATGT
BsrDI 1783 CATTGC GCAATG(2/0)
BsrFI 78 GCCGGC [GA]^CCGG[CT]
Cfr10I 78 GCCGGC [GA]^CCGG[CT]
DraIII 582 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2927 AGCGCT AGC^GCT
KasI 104 GGCGCC G^GCGCC
KpnI 4840 GGTACC GGTAC^C
MaeII 1140 ACGT A^CGT
MluNI 4579 TGGCCA TGG^CCA
MscI 4579 TGGCCA TGG^CCA
NaeI 78 GCCGGC GCC^GGC
NarI 104 GGCGCC GG^CGCC
NcoI 616 CCATGG C^CATGG
NgoMIV 78 GCCGGC G^CCGGC
NheI 3048 GCTAGC G^CTAGC
PaeR7I 912 CTCGAG C^TCGAG
PshAI 1576 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 3917 CAGCTG CAG^CTG
SacI 577 GAGCTC GAGCT^C
SapI 749 GCTCTTC GCTCTTC(1/4)
SexAI 286 ACCTGGT A^CC[AT]GGT
SfoI 104 GGCGCC GGC^GCC
SpeI 4885 ACTAGT A^CTAGT
SspI 3842 AATATT AAT^ATT
StuI 4363 AGGCCT AGG^CCT
XbaI 1838 TCTAGA T^CTAGA
XcmI 1808 CCACTCCTGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 912 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 149 CGCG CG^CG
155 CGCG
Asp700I 397 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4476 GAAGAGGTTC
AspI 285 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1136 GACGACGTC
AvrII 1788 CCTAGG C^CTAGG
3381 CCTAGG
BglII 4342 AGATCT A^GATCT
4450 AGATCT
BlnI 1788 CCTAGG C^CTAGG
3381 CCTAGG
BlpI 87 GCTCAGC GC^T[ACGT]AGC
4426 GCTGAGC
Bpu1102I 87 GCTCAGC GC^T[ACGT]AGC
4426 GCTGAGC
BsaI 3317 GAGACC GGTCTC(1/5)
2869 GGTCTC
BsmI 4441 GAATGC GAATGC(1/-1)
4143 GCATTC
BsrBI 85 CCGCTC CCGCTC(-3/-3)
21 GAGCGG
BssSI 582 CACGAG CACGAG(-5/-1)
4741 CACGAG
BstAPI 1251 GCATCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1778 GCATGCATTGC
BstEII 782 GGTTACC G^GT[ACGT]ACC
2542 GGTAACC
BstPI 782 GGTTACC G^GT[ACGT]ACC
2542 GGTAACC
BstUI 149 CGCG CG^CG
155 CGCG
CelII 87 GCTCAGC GC^T[ACGT]AGC
4426 GCTGAGC
EaeI 47 CGGCCA [CT]^GGCC[GA]
4579 TGGCCA
EcoO65I 782 GGTTACC G^GT[ACGT]ACC
2542 GGTAACC
EcoRV 606 GATATC GAT^ATC
1179 GATATC
HgaI 12 GCGTC GACGC(5/10)
3962 GCGTC
HindIII 2181 AAGCTT A^AGCTT
3229 AAGCTT
MspA1I 433 CCGCTG C[AC]G^C[GT]G
3917 CAGCTG
MvnI 149 CGCG CG^CG
155 CGCG
PflFI 285 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1136 GACGACGTC
PstI 591 CTGCAG CTGCA^G
792 CTGCAG
SmaI 129 CCCGGG CCC^GGG
717 CCCGGG
SphI 1778 GCATGC GCATG^C
4004 GCATGC
Tth111I 285 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1136 GACGACGTC
XmaCI 129 CCCGGG C^CCGGG
717 CCCGGG
XmaI 129 CCCGGG C^CCGGG
717 CCCGGG
XmnI 397 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4476 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BfrI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI Eco81I EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII