##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI BbeI
BbrPI BcgI BciVI BfrI BglI
BglII BsaAI BsiWI BspHI BssHII
BssSI BstBI CpoI DraI DrdI
Eam1105I EcoRV FseI HindIII KasI
KpnI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 3247 TCCGGA T^CCGGA
AcsI 4896 GAATTC [GA]^AATT[CT]
ApaI 1704 GGGCCC GGGCC^C
ApoI 4896 GAATTC [GA]^AATT[CT]
AspI 944 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 562 TGCGCA TGC^GCA
AvrII 1712 CCTAGG C^CTAGG
BbvCI 1630 CCTCAGC CCTCAGC(-5/-2)
BclI 1946 TGATCA T^GATCA
BlnI 1712 CCTAGG C^CTAGG
BlpI 614 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 614 GCTGAGC GC^T[ACGT]AGC
BsaBI 3703 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3247 TCCGGA T^CCGGA
BsiEI 427 CGGCCG CG[GA][CT]^CG
BsmBI 3227 GAGACG CGTCTC(1/5)
BspDI 1641 ATCGAT AT^CGAT
BspEI 3247 TCCGGA T^CCGGA
BsrBI 744 GAGCGG CCGCTC(-3/-3)
BsrFI 2325 ACCGGC [GA]^CCGG[CT]
Bst1107I 3263 GTATAC GTA^TAC
BstEII 4320 GGTCACC G^GT[ACGT]ACC
BstPI 4320 GGTCACC G^GT[ACGT]ACC
BstZ17I 3263 GTATAC GTA^TAC
CelII 614 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2325 ACCGGC [GA]^CCGG[CT]
ClaI 1641 ATCGAT AT^CGAT
DraIII 3633 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 427 CGGCCG C^GGCCG
EclXI 427 CGGCCG C^GGCCG
Eco52I 427 CGGCCG C^GGCCG
EcoO65I 4320 GGTCACC G^GT[ACGT]ACC
EcoRI 4896 GAATTC G^AATTC
FbaI 1946 TGATCA T^GATCA
FspI 562 TGCGCA TGC^GCA
HpaI 2357 GTTAAC GTT^AAC
KspI 133 CCGCGG CCGC^GG
MamI 3703 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3247 TCCGGA T^CCGGA
NheI 4905 GCTAGC G^CTAGC
PflFI 944 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 902 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1611 GAGCTC GAGCT^C
SacII 133 CCGCGG CCGC^GG
SapI 4843 GAAGAGC GCTCTTC(1/4)
SphI 499 GCATGC GCATG^C
Tth111I 944 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 902 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 165 TCTAGA T^CTAGA
XcmI 2395 CCACCCTCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 2631 GGCGTC G[GA]^CG[CT]C
3742 GGCGTC
Aor51HI 3064 AGCGCT AGC^GCT
4472 AGCGCT
BamHI 890 GGATCC G^GATCC
2542 GGATCC
BanI 3016 GGTGCC G^G[CT][GA]CC
4786 GGTGCC
BsaHI 2631 GGCGTC G[GA]^CG[CT]C
3742 GGCGTC
BsmI 1517 GAATGC GAATGC(1/-1)
236 GCATTC
Bsp1407I 2891 TGTACA T^GTACA
4644 TGTACA
BsrDI 283 CATTGC GCAATG(2/0)
4728 GCAATG
BsrGI 2891 TGTACA T^GTACA
4644 TGTACA
BstAPI 2242 GCATTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3211 GCATCTGCTGC
Eco47III 3064 AGCGCT AGC^GCT
4472 AGCGCT
EcoNI 662 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1712 CCTAGGAGAGG
EcoT22I 497 ATGCAT ATGCA^T
1519 ATGCAT
Hin1I 2631 GGCGTC G[GA]^CG[CT]C
3742 GGCGTC
NsiI 497 ATGCAT ATGCA^T
1519 ATGCAT
SalI 941 GTCGAC G^TCGAC
1698 GTCGAC
SmaI 582 CCCGGG CCC^GGG
960 CCCGGG
SspBI 2891 TGTACA T^GTACA
4644 TGTACA
StuI 1345 AGGCCT AGG^CCT
4108 AGGCCT
XmaCI 582 CCCGGG C^CCGGG
960 CCCGGG
XmaI 582 CCCGGG C^CCGGG
960 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AfaI AflIII
AluI AlwI AlwNI AocI AspHI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I SbfI ScaI ScrFI SfaNI
SfcI SmlI Sse8387I StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII