################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspEI BbeI BbrPI BcgI BciVI BfrI BglI BglII BsaAI BsiWI BspHI BssHII BssSI BstBI CpoI DraI DrdI Eam1105I EcoRV FseI HindIII KasI KpnI MfeI MluI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 3247 TCCGGA T^CCGGA AcsI 4896 GAATTC [GA]^AATT[CT] ApaI 1704 GGGCCC GGGCC^C ApoI 4896 GAATTC [GA]^AATT[CT] AspI 944 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 562 TGCGCA TGC^GCA AvrII 1712 CCTAGG C^CTAGG BbvCI 1630 CCTCAGC CCTCAGC(-5/-2) BclI 1946 TGATCA T^GATCA BlnI 1712 CCTAGG C^CTAGG BlpI 614 GCTGAGC GC^T[ACGT]AGC Bpu1102I 614 GCTGAGC GC^T[ACGT]AGC BsaBI 3703 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 3247 TCCGGA T^CCGGA BsiEI 427 CGGCCG CG[GA][CT]^CG BsmBI 3227 GAGACG CGTCTC(1/5) BspDI 1641 ATCGAT AT^CGAT BspEI 3247 TCCGGA T^CCGGA BsrBI 744 GAGCGG CCGCTC(-3/-3) BsrFI 2325 ACCGGC [GA]^CCGG[CT] Bst1107I 3263 GTATAC GTA^TAC BstEII 4320 GGTCACC G^GT[ACGT]ACC BstPI 4320 GGTCACC G^GT[ACGT]ACC BstZ17I 3263 GTATAC GTA^TAC CelII 614 GCTGAGC GC^T[ACGT]AGC Cfr10I 2325 ACCGGC [GA]^CCGG[CT] ClaI 1641 ATCGAT AT^CGAT DraIII 3633 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 427 CGGCCG C^GGCCG EclXI 427 CGGCCG C^GGCCG Eco52I 427 CGGCCG C^GGCCG EcoO65I 4320 GGTCACC G^GT[ACGT]ACC EcoRI 4896 GAATTC G^AATTC FbaI 1946 TGATCA T^GATCA FspI 562 TGCGCA TGC^GCA HpaI 2357 GTTAAC GTT^AAC KspI 133 CCGCGG CCGC^GG MamI 3703 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 3247 TCCGGA T^CCGGA NheI 4905 GCTAGC G^CTAGC PflFI 944 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 902 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 1611 GAGCTC GAGCT^C SacII 133 CCGCGG CCGC^GG SapI 4843 GAAGAGC GCTCTTC(1/4) SphI 499 GCATGC GCATG^C Tth111I 944 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 902 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 165 TCTAGA T^CTAGA XcmI 2395 CCACCCTCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 2631 GGCGTC G[GA]^CG[CT]C 3742 GGCGTC Aor51HI 3064 AGCGCT AGC^GCT 4472 AGCGCT BamHI 890 GGATCC G^GATCC 2542 GGATCC BanI 3016 GGTGCC G^G[CT][GA]CC 4786 GGTGCC BsaHI 2631 GGCGTC G[GA]^CG[CT]C 3742 GGCGTC BsmI 1517 GAATGC GAATGC(1/-1) 236 GCATTC Bsp1407I 2891 TGTACA T^GTACA 4644 TGTACA BsrDI 283 CATTGC GCAATG(2/0) 4728 GCAATG BsrGI 2891 TGTACA T^GTACA 4644 TGTACA BstAPI 2242 GCATTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3211 GCATCTGCTGC Eco47III 3064 AGCGCT AGC^GCT 4472 AGCGCT EcoNI 662 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1712 CCTAGGAGAGG EcoT22I 497 ATGCAT ATGCA^T 1519 ATGCAT Hin1I 2631 GGCGTC G[GA]^CG[CT]C 3742 GGCGTC NsiI 497 ATGCAT ATGCA^T 1519 ATGCAT SalI 941 GTCGAC G^TCGAC 1698 GTCGAC SmaI 582 CCCGGG CCC^GGG 960 CCCGGG SspBI 2891 TGTACA T^GTACA 4644 TGTACA StuI 1345 AGGCCT AGG^CCT 4108 AGGCCT XmaCI 582 CCCGGG C^CCGGG 960 CCCGGG XmaI 582 CCCGGG C^CCGGG 960 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccI AccII AciI AfaI AflIII AluI AlwI AlwNI AocI AspHI AvaI AvaII BalI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I SbfI ScaI ScrFI SfaNI SfcI SmlI Sse8387I StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII