##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       BbeI        
BbrPI       BcgI        BciVI       BfrI        BglI        
BglII       BsaAI       BsiWI       BspHI       BssHII      
BssSI       BstBI       CpoI        DraI        DrdI        
Eam1105I    EcoRV       FseI        HindIII     KasI        
KpnI        MfeI        MluI        MunI        NaeI        
NarI        NdeI        NgoMIV      NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SspI        SwaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII       3247  TCCGGA               T^CCGGA
AcsI         4896  GAATTC               [GA]^AATT[CT]
ApaI         1704  GGGCCC               GGGCC^C
ApoI         4896  GAATTC               [GA]^AATT[CT]
AspI          944  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         562  TGCGCA               TGC^GCA
AvrII        1712  CCTAGG               C^CTAGG
BbvCI        1630  CCTCAGC              CCTCAGC(-5/-2)
BclI         1946  TGATCA               T^GATCA
BlnI         1712  CCTAGG               C^CTAGG
BlpI          614  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      614  GCTGAGC              GC^T[ACGT]AGC
BsaBI        3703  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        3247  TCCGGA               T^CCGGA
BsiEI         427  CGGCCG               CG[GA][CT]^CG
BsmBI        3227  GAGACG               CGTCTC(1/5)
BspDI        1641  ATCGAT               AT^CGAT
BspEI        3247  TCCGGA               T^CCGGA
BsrBI         744  GAGCGG               CCGCTC(-3/-3)
BsrFI        2325  ACCGGC               [GA]^CCGG[CT]
Bst1107I     3263  GTATAC               GTA^TAC
BstEII       4320  GGTCACC              G^GT[ACGT]ACC
BstPI        4320  GGTCACC              G^GT[ACGT]ACC
BstZ17I      3263  GTATAC               GTA^TAC
CelII         614  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       2325  ACCGGC               [GA]^CCGG[CT]
ClaI         1641  ATCGAT               AT^CGAT
DraIII       3633  CACATAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          427  CGGCCG               C^GGCCG
EclXI         427  CGGCCG               C^GGCCG
Eco52I        427  CGGCCG               C^GGCCG
EcoO65I      4320  GGTCACC              G^GT[ACGT]ACC
EcoRI        4896  GAATTC               G^AATTC
FbaI         1946  TGATCA               T^GATCA
FspI          562  TGCGCA               TGC^GCA
HpaI         2357  GTTAAC               GTT^AAC
KspI          133  CCGCGG               CCGC^GG
MamI         3703  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         3247  TCCGGA               T^CCGGA
NheI         4905  GCTAGC               G^CTAGC
PflFI         944  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         902  CCACCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI         1611  GAGCTC               GAGCT^C
SacII         133  CCGCGG               CCGC^GG
SapI         4843  GAAGAGC              GCTCTTC(1/4)
SphI          499  GCATGC               GCATG^C
Tth111I       944  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        902  CCACCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          165  TCTAGA               T^CTAGA
XcmI         2395  CCACCCTCACTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcyI         2631  GGCGTC               G[GA]^CG[CT]C
             3742  GGCGTC              
Aor51HI      3064  AGCGCT               AGC^GCT
             4472  AGCGCT              
BamHI         890  GGATCC               G^GATCC
             2542  GGATCC              
BanI         3016  GGTGCC               G^G[CT][GA]CC
             4786  GGTGCC              
BsaHI        2631  GGCGTC               G[GA]^CG[CT]C
             3742  GGCGTC              
BsmI         1517  GAATGC               GAATGC(1/-1)
              236  GCATTC              
Bsp1407I     2891  TGTACA               T^GTACA
             4644  TGTACA              
BsrDI         283  CATTGC               GCAATG(2/0)
             4728  GCAATG              
BsrGI        2891  TGTACA               T^GTACA
             4644  TGTACA              
BstAPI       2242  GCATTGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3211  GCATCTGCTGC         
Eco47III     3064  AGCGCT               AGC^GCT
             4472  AGCGCT              
EcoNI         662  CCTCAGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1712  CCTAGGAGAGG         
EcoT22I       497  ATGCAT               ATGCA^T
             1519  ATGCAT              
Hin1I        2631  GGCGTC               G[GA]^CG[CT]C
             3742  GGCGTC              
NsiI          497  ATGCAT               ATGCA^T
             1519  ATGCAT              
SalI          941  GTCGAC               G^TCGAC
             1698  GTCGAC              
SmaI          582  CCCGGG               CCC^GGG
              960  CCCGGG              
SspBI        2891  TGTACA               T^GTACA
             4644  TGTACA              
StuI         1345  AGGCCT               AGG^CCT
             4108  AGGCCT              
XmaCI         582  CCCGGG               C^CCGGG
              960  CCCGGG              
XmaI          582  CCCGGG               C^CCGGG
              960  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccI        AccII       AciI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        AspHI       
AvaI        AvaII       BalI        BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspLU11I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      SbfI        ScaI        ScrFI       SfaNI       
SfcI        SmlI        Sse8387I    StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII