##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspI
AviII BbeI BcgI BciVI BglII
BsaBI BsiWI BsmI BspDI BspHI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FseI FspI KasI KpnI KspI
MamI MfeI MluI MunI NarI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SfoI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 2157 GACGTC GACGT^C
AccI 863 GTCTAC GT^[AC][GT]AC
AccIII 937 TCCGGA T^CCGGA
AflII 5290 CTTAAG C^TTAAG
AhdI 291 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 291 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 914 CACGTG CAC^GTG
BfrI 5290 CTTAAG C^TTAAG
BlpI 4664 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4664 GCTGAGC GC^T[ACGT]AGC
BsaAI 914 CACGTG [CT]AC^GT[GA]
BseAI 937 TCCGGA T^CCGGA
BsiEI 687 CGGTCG CG[GA][CT]^CG
BspEI 937 TCCGGA T^CCGGA
BspLU11I 4438 ACATGT A^CATGT
BsrBI 3841 CCGCTC CCGCTC(-3/-3)
CelII 4664 GCTGAGC GC^T[ACGT]AGC
DraI 5130 TTTAAA TTT^AAA
Eam1105I 291 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1085 GAATTC G^AATTC
HindIII 1314 AAGCTT A^AGCTT
HpaI 4786 GTTAAC GTT^AAC
MroI 937 TCCGGA T^CCGGA
NaeI 2447 GCCGGC GCC^GGC
NgoMIV 2447 GCCGGC G^CCGGC
NheI 790 GCTAGC G^CTAGC
PmaCI 914 CACGTG CAC^GTG
PmlI 914 CACGTG CAC^GTG
PshAI 2308 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 3571 GCTCTTC GCTCTTC(1/4)
SfiI 2167 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2162 CCCGGG CCC^GGG
StuI 718 AGGCCT AGG^CCT
XmaCI 2162 CCCGGG C^CCGGG
XmaI 2162 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 2150 ACGTGT A^C[GA][CT]GT
4438 ACATGT
Alw44I 1672 GTGCAC G^TGCAC
3233 GTGCAC
ApaLI 1672 GTGCAC G^TGCAC
3233 GTGCAC
BbvCI 245 GCTGAGG CCTCAGC(-5/-2)
497 GCTGAGG
BclI 2368 TGATCA T^GATCA
3453 TGATCA
BsaWI 937 TCCGGA [AT]^CCGG[AT]
3343 TCCGGT
BsmBI 2602 CGTCTC CGTCTC(1/5)
2139 GAGACG
Bsp1407I 447 TGTACA T^GTACA
1221 TGTACA
BsrFI 797 ACCGGC [GA]^CCGG[CT]
2447 GCCGGC
BsrGI 447 TGTACA T^GTACA
1221 TGTACA
BssSI 869 CACGAG CACGAG(-5/-1)
4619 CACGAG
Cfr10I 797 ACCGGC [GA]^CCGG[CT]
2447 GCCGGC
EcoNI 3212 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3667 CCTCTGGAAGG
FbaI 2368 TGATCA T^GATCA
3453 TGATCA
HincII 1115 GTCAAC GT[CT]^[GA]AC
4786 GTTAAC
HindII 1115 GTCAAC GT[CT]^[GA]AC
4786 GTTAAC
SacI 941 GAGCTC GAGCT^C
5295 GAGCTC
SbfI 3718 CCTGCAGG CCTGCA^GG
3785 CCTGCAGG
ScaI 2646 AGTACT AGT^ACT
4098 AGTACT
SexAI 1110 ACCAGGT A^CC[AT]GGT
1340 ACCTGGT
Sse8387I 3718 CCTGCAGG CCTGCA^GG
3785 CCTGCAGG
SspBI 447 TGTACA T^GTACA
1221 TGTACA
TaqI 715 TCGA T^CGA
4567 TCGA
TthHB8I 715 TCGA T^CGA
4567 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII AvrII
BalI BamHI BanI BanII BbsI
BbvI BcnI BfaI BglI BlnI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII