################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## Acc65I AclI AgeI Aor51HI AscI AseI AsnI Asp700I Asp718I AspI AviII BbeI BcgI BciVI BglII BsaBI BsiWI BsmI BspDI BspHI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FseI FspI KasI KpnI KspI MamI MfeI MluI MunI NarI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SfoI SfuI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 2157 GACGTC GACGT^C AccI 863 GTCTAC GT^[AC][GT]AC AccIII 937 TCCGGA T^CCGGA AflII 5290 CTTAAG C^TTAAG AhdI 291 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 291 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 914 CACGTG CAC^GTG BfrI 5290 CTTAAG C^TTAAG BlpI 4664 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4664 GCTGAGC GC^T[ACGT]AGC BsaAI 914 CACGTG [CT]AC^GT[GA] BseAI 937 TCCGGA T^CCGGA BsiEI 687 CGGTCG CG[GA][CT]^CG BspEI 937 TCCGGA T^CCGGA BspLU11I 4438 ACATGT A^CATGT BsrBI 3841 CCGCTC CCGCTC(-3/-3) CelII 4664 GCTGAGC GC^T[ACGT]AGC DraI 5130 TTTAAA TTT^AAA Eam1105I 291 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1085 GAATTC G^AATTC HindIII 1314 AAGCTT A^AGCTT HpaI 4786 GTTAAC GTT^AAC MroI 937 TCCGGA T^CCGGA NaeI 2447 GCCGGC GCC^GGC NgoMIV 2447 GCCGGC G^CCGGC NheI 790 GCTAGC G^CTAGC PmaCI 914 CACGTG CAC^GTG PmlI 914 CACGTG CAC^GTG PshAI 2308 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 3571 GCTCTTC GCTCTTC(1/4) SfiI 2167 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 2162 CCCGGG CCC^GGG StuI 718 AGGCCT AGG^CCT XmaCI 2162 CCCGGG C^CCGGG XmaI 2162 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 2150 ACGTGT A^C[GA][CT]GT 4438 ACATGT Alw44I 1672 GTGCAC G^TGCAC 3233 GTGCAC ApaLI 1672 GTGCAC G^TGCAC 3233 GTGCAC BbvCI 245 GCTGAGG CCTCAGC(-5/-2) 497 GCTGAGG BclI 2368 TGATCA T^GATCA 3453 TGATCA BsaWI 937 TCCGGA [AT]^CCGG[AT] 3343 TCCGGT BsmBI 2602 CGTCTC CGTCTC(1/5) 2139 GAGACG Bsp1407I 447 TGTACA T^GTACA 1221 TGTACA BsrFI 797 ACCGGC [GA]^CCGG[CT] 2447 GCCGGC BsrGI 447 TGTACA T^GTACA 1221 TGTACA BssSI 869 CACGAG CACGAG(-5/-1) 4619 CACGAG Cfr10I 797 ACCGGC [GA]^CCGG[CT] 2447 GCCGGC EcoNI 3212 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3667 CCTCTGGAAGG FbaI 2368 TGATCA T^GATCA 3453 TGATCA HincII 1115 GTCAAC GT[CT]^[GA]AC 4786 GTTAAC HindII 1115 GTCAAC GT[CT]^[GA]AC 4786 GTTAAC SacI 941 GAGCTC GAGCT^C 5295 GAGCTC SbfI 3718 CCTGCAGG CCTGCA^GG 3785 CCTGCAGG ScaI 2646 AGTACT AGT^ACT 4098 AGTACT SexAI 1110 ACCAGGT A^CC[AT]GGT 1340 ACCTGGT Sse8387I 3718 CCTGCAGG CCTGCA^GG 3785 CCTGCAGG SspBI 447 TGTACA T^GTACA 1221 TGTACA TaqI 715 TCGA T^CGA 4567 TCGA TthHB8I 715 TCGA T^CGA 4567 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII AvrII BalI BamHI BanI BanII BbsI BbvI BcnI BfaI BglI BlnI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII