##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AclI AflIII AgeI Aor51HI
AscI AseI AsnI BamHI BbrPI
BglII BsaAI BsaBI BsiWI BsmBI
Bsp1407I BspHI BspLU11I BsrGI BssHII
CpoI DrdI Eco47III EcoRV EcoT22I
FseI HincII HindII HpaI KspI
MamI MluI NheI NruI NsiI
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I RcaI RsrII SacII
SalI SbfI ScaI SfiI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 4736 GGTACC G^GTACC
AccIII 2459 TCCGGA T^CCGGA
AflII 4783 CTTAAG C^TTAAG
AhdI 2242 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 849 GTGCAC G^TGCAC
AocI 4271 CCTCAGG CC^T[ACGT]AGG
ApaI 931 GGGCCC GGGCC^C
ApaLI 849 GTGCAC G^TGCAC
Asp718I 4736 GGTACC G^GTACC
AspEI 2242 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 550 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2507 TGCGCA TGC^GCA
AvrII 2268 CCTAGG C^CTAGG
BbvCI 1576 GCTGAGG CCTCAGC(-5/-2)
BciVI 4215 GGATAC GTATCC(6/5)
BclI 3435 TGATCA T^GATCA
BfrI 4783 CTTAAG C^TTAAG
BlnI 2268 CCTAGG C^CTAGG
BlpI 1414 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1414 GCTCAGC GC^T[ACGT]AGC
BseAI 2459 TCCGGA T^CCGGA
BspDI 521 ATCGAT AT^CGAT
BspEI 2459 TCCGGA T^CCGGA
BssSI 3584 CACGAG CACGAG(-5/-1)
BstBI 1367 TTCGAA TT^CGAA
BstEII 3889 GGTGACC G^GT[ACGT]ACC
BstPI 3889 GGTGACC G^GT[ACGT]ACC
Bsu36I 4271 CCTCAGG CC^T[ACGT]AGG
CelII 1414 GCTCAGC GC^T[ACGT]AGC
ClaI 521 ATCGAT AT^CGAT
DraIII 2511 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2242 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4271 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3889 GGTGACC G^GT[ACGT]ACC
EcoRI 712 GAATTC G^AATTC
FbaI 3435 TGATCA T^GATCA
FspI 2507 TGCGCA TGC^GCA
KpnI 4736 GGTACC GGTAC^C
MfeI 4450 CAATTG C^AATTG
MroI 2459 TCCGGA T^CCGGA
MunI 4450 CAATTG C^AATTG
NaeI 351 GCCGGC GCC^GGC
NdeI 2783 CATATG CA^TATG
NgoMIV 351 GCCGGC G^CCGGC
NotI 90 GCGGCCGC GC^GGCCGC
NspV 1367 TTCGAA TT^CGAA
PaeR7I 3028 CTCGAG C^TCGAG
PflFI 550 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3693 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 2243 ACCAGGT A^CC[AT]GGT
SfuI 1367 TTCGAA TT^CGAA
SgrAI 1555 CACCGGCG C[GA]^CCGG[CT]G
SspI 1394 AATATT AAT^ATT
Tth111I 550 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3028 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Asp700I 282 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
360 GAACGCCTTC
BbeI 387 GGCGCC GGCGC^C
674 GGCGCC
BsaI 658 GAGACC GGTCTC(1/5)
3382 GGTCTC
BsmI 2829 GAATGC GAATGC(1/-1)
3589 GCATTC
EagI 59 CGGCCG C^GGCCG
91 CGGCCG
EclXI 59 CGGCCG C^GGCCG
91 CGGCCG
Eco52I 59 CGGCCG C^GGCCG
91 CGGCCG
EcoNI 429 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1983 CCTGTCTCAGG
HindIII 3127 AAGCTT A^AGCTT
4151 AAGCTT
KasI 387 GGCGCC G^GCGCC
674 GGCGCC
MaeII 727 ACGT A^CGT
5124 ACGT
NarI 387 GGCGCC GG^CGCC
674 GGCGCC
PvuI 173 CGATCG CGAT^CG
3094 CGATCG
SapI 396 GCTCTTC GCTCTTC(1/4)
3465 GCTCTTC
SfoI 387 GGCGCC GGC^GCC
674 GGCGCC
StuI 2502 AGGCCT AGG^CCT
4268 AGGCCT
XbaI 4684 TCTAGA T^CTAGA
4859 TCTAGA
XmnI 282 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
360 GAACGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI Bst1107I
BstAPI BstF5I BstNI BstUI BstXI
BstYI BstZ17I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII