################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BciVI BlnI BsaBI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI HpaI KpnI KspI MamI MfeI MluI MroI MunI NheI NotI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SfiI SfuI SgrAI SnaBI SpeI SphI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### AhdI 3620 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 3620 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 1763 GGCGCC GGCGC^C BcgI 2083 CGAGTTCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 4622 TGATCA T^GATCA BsmBI 3589 CGTCTC CGTCTC(1/5) BspHI 301 TCATGA T^CATGA BstXI 465 CCAGAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Eam1105I 3620 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 4075 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 4622 TGATCA T^GATCA KasI 1763 GGCGCC G^GCGCC NaeI 131 GCCGGC GCC^GGC NarI 1763 GGCGCC GG^CGCC NcoI 1579 CCATGG C^CATGG NdeI 4650 CATATG CA^TATG NgoMIV 131 GCCGGC G^CCGGC NruI 3706 TCGCGA TCG^CGA RcaI 301 TCATGA T^CATGA SapI 2050 GCTCTTC GCTCTTC(1/4) ScaI 3654 AGTACT AGT^ACT SfoI 1763 GGCGCC GGC^GCC SmaI 3442 CCCGGG CCC^GGG XbaI 1018 TCTAGA T^CTAGA XmaCI 3442 CCCGGG C^CCGGG XmaI 3442 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflII 701 CTTAAG C^TTAAG 710 CTTAAG Alw44I 1148 GTGCAC G^TGCAC 4444 GTGCAC ApaI 1755 GGGCCC GGGCC^C 4024 GGGCCC ApaLI 1148 GTGCAC G^TGCAC 4444 GTGCAC BbrPI 1829 CACGTG CAC^GTG 3694 CACGTG BfrI 701 CTTAAG C^TTAAG 710 CTTAAG BglI 19 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 188 GCCAAGGAGGC BglII 2872 AGATCT A^GATCT 4065 AGATCT BspLU11I 4585 ACATGT A^CATGT 4615 ACATGT BssHII 197 GCGCGC G^CGCGC 2364 GCGCGC BstEII 250 GGTTACC G^GT[ACGT]ACC 847 GGTCACC BstPI 250 GGTTACC G^GT[ACGT]ACC 847 GGTCACC EcoO65I 250 GGTTACC G^GT[ACGT]ACC 847 GGTCACC HincII 3632 GTCAAC GT[CT]^[GA]AC 3791 GTTGAC HindII 3632 GTCAAC GT[CT]^[GA]AC 3791 GTTGAC NspI 4585 ACATGT [GA]CATG^[CT] 4615 ACATGT PaeR7I 382 CTCGAG C^TCGAG 2594 CTCGAG PmaCI 1829 CACGTG CAC^GTG 3694 CACGTG PmlI 1829 CACGTG CAC^GTG 3694 CACGTG SacI 215 GAGCTC GAGCT^C 4430 GAGCTC SbfI 1328 CCTGCAGG CCTGCA^GG 2170 CCTGCAGG SexAI 801 ACCAGGT A^CC[AT]GGT 2256 ACCTGGT Sse8387I 1328 CCTGCAGG CCTGCA^GG 2170 CCTGCAGG SspI 749 AATATT AAT^ATT 784 AATATT XcmI 247 CCAGGTTACCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4451 CCATAGCAGCCCTGG XhoI 382 CTCGAG C^TCGAG 2594 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII