##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BciVI BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BsmI BspDI
BspEI Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FseI FspI HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NheI NotI NsiI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SfiI SfuI SgrAI SnaBI
SpeI SphI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AhdI 3620 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3620 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1763 GGCGCC GGCGC^C
BcgI 2083 CGAGTTCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4622 TGATCA T^GATCA
BsmBI 3589 CGTCTC CGTCTC(1/5)
BspHI 301 TCATGA T^CATGA
BstXI 465 CCAGAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 3620 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4075 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 4622 TGATCA T^GATCA
KasI 1763 GGCGCC G^GCGCC
NaeI 131 GCCGGC GCC^GGC
NarI 1763 GGCGCC GG^CGCC
NcoI 1579 CCATGG C^CATGG
NdeI 4650 CATATG CA^TATG
NgoMIV 131 GCCGGC G^CCGGC
NruI 3706 TCGCGA TCG^CGA
RcaI 301 TCATGA T^CATGA
SapI 2050 GCTCTTC GCTCTTC(1/4)
ScaI 3654 AGTACT AGT^ACT
SfoI 1763 GGCGCC GGC^GCC
SmaI 3442 CCCGGG CCC^GGG
XbaI 1018 TCTAGA T^CTAGA
XmaCI 3442 CCCGGG C^CCGGG
XmaI 3442 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflII 701 CTTAAG C^TTAAG
710 CTTAAG
Alw44I 1148 GTGCAC G^TGCAC
4444 GTGCAC
ApaI 1755 GGGCCC GGGCC^C
4024 GGGCCC
ApaLI 1148 GTGCAC G^TGCAC
4444 GTGCAC
BbrPI 1829 CACGTG CAC^GTG
3694 CACGTG
BfrI 701 CTTAAG C^TTAAG
710 CTTAAG
BglI 19 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
188 GCCAAGGAGGC
BglII 2872 AGATCT A^GATCT
4065 AGATCT
BspLU11I 4585 ACATGT A^CATGT
4615 ACATGT
BssHII 197 GCGCGC G^CGCGC
2364 GCGCGC
BstEII 250 GGTTACC G^GT[ACGT]ACC
847 GGTCACC
BstPI 250 GGTTACC G^GT[ACGT]ACC
847 GGTCACC
EcoO65I 250 GGTTACC G^GT[ACGT]ACC
847 GGTCACC
HincII 3632 GTCAAC GT[CT]^[GA]AC
3791 GTTGAC
HindII 3632 GTCAAC GT[CT]^[GA]AC
3791 GTTGAC
NspI 4585 ACATGT [GA]CATG^[CT]
4615 ACATGT
PaeR7I 382 CTCGAG C^TCGAG
2594 CTCGAG
PmaCI 1829 CACGTG CAC^GTG
3694 CACGTG
PmlI 1829 CACGTG CAC^GTG
3694 CACGTG
SacI 215 GAGCTC GAGCT^C
4430 GAGCTC
SbfI 1328 CCTGCAGG CCTGCA^GG
2170 CCTGCAGG
SexAI 801 ACCAGGT A^CC[AT]GGT
2256 ACCTGGT
Sse8387I 1328 CCTGCAGG CCTGCA^GG
2170 CCTGCAGG
SspI 749 AATATT AAT^ATT
784 AATATT
XcmI 247 CCAGGTTACCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4451 CCATAGCAGCCCTGG
XhoI 382 CTCGAG C^TCGAG
2594 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII