##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
Acc65I AccII AgeI Aor51HI AscI
AseI AsnI Asp718I BcgI BclI
BsiWI BsmBI BspDI BspHI BssHII
Bst1107I BstBI BstUI BstZ17I ClaI
CpoI Eco47III FbaI KpnI KspI
MfeI MluI MunI MvnI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 1347 GACGTC GACGT^C
AclI 2025 AACGTT AA^CGTT
AflII 3096 CTTAAG C^TTAAG
AflIII 2744 ACATGT A^C[GA][CT]GT
AhdI 1970 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 836 GTGCAC G^TGCAC
ApaI 2519 GGGCCC GGGCC^C
ApaLI 836 GTGCAC G^TGCAC
AspEI 1970 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 7322 TGCGCA TGC^GCA
AvrII 82 CCTAGG C^CTAGG
BbrPI 2275 CACGTG CAC^GTG
BciVI 3703 GTATCC GTATCC(6/5)
BfrI 3096 CTTAAG C^TTAAG
BglII 5405 AGATCT A^GATCT
BlnI 82 CCTAGG C^CTAGG
BsaBI 1470 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 56 CGGCCG CG[GA][CT]^CG
BspLU11I 2744 ACATGT A^CATGT
DrdI 4679 GACAGTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 56 CGGCCG C^GGCCG
Eam1105I 1970 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 56 CGGCCG C^GGCCG
Eco52I 56 CGGCCG C^GGCCG
EcoRI 5200 GAATTC G^AATTC
FspI 7322 TGCGCA TGC^GCA
HpaI 5492 GTTAAC GTT^AAC
MamI 1470 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 4811 CATATG CA^TATG
PmaCI 2275 CACGTG CAC^GTG
PmlI 2275 CACGTG CAC^GTG
PshAI 3054 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2025 AACGTT AA^CGTT
SfiI 7148 GGCCATAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 4507 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 557 TCCGGA T^CCGGA
2168 TCCGGA
AocI 3251 CCTCAGG CC^T[ACGT]AGG
3714 CCTCAGG
AspI 395 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
455 GACACAGTC
BalI 3949 TGGCCA TGG^CCA
5424 TGGCCA
BamHI 5482 GGATCC G^GATCC
6497 GGATCC
BbeI 1482 GGCGCC GGCGC^C
7011 GGCGCC
BbvCI 3378 CCTCAGC CCTCAGC(-5/-2)
6716 CCTCAGC
BglI 4275 GCCAGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
7149 GCCATAGTGGC
BsaWI 557 TCCGGA [AT]^CCGG[AT]
2168 TCCGGA
BseAI 557 TCCGGA T^CCGGA
2168 TCCGGA
BspEI 557 TCCGGA T^CCGGA
2168 TCCGGA
BstEII 2495 GGTTACC G^GT[ACGT]ACC
4844 GGTAACC
BstPI 2495 GGTTACC G^GT[ACGT]ACC
4844 GGTAACC
Bsu36I 3251 CCTCAGG CC^T[ACGT]AGG
3714 CCTCAGG
DraI 2815 TTTAAA TTT^AAA
4858 TTTAAA
Eco81I 3251 CCTCAGG CC^T[ACGT]AGG
3714 CCTCAGG
EcoO65I 2495 GGTTACC G^GT[ACGT]ACC
4844 GGTAACC
FseI 53 GGCCGGCC GGCCGG^CC
57 GGCCGGCC
HgaI 4750 GACGC GACGC(5/10)
2181 GCGTC
HincII 4955 GTTGAC GT[CT]^[GA]AC
5492 GTTAAC
HindII 4955 GTTGAC GT[CT]^[GA]AC
5492 GTTAAC
KasI 1482 GGCGCC G^GCGCC
7011 GGCGCC
MluNI 3949 TGGCCA TGG^CCA
5424 TGGCCA
MroI 557 TCCGGA T^CCGGA
2168 TCCGGA
MscI 3949 TGGCCA TGG^CCA
5424 TGGCCA
NarI 1482 GGCGCC GG^CGCC
7011 GGCGCC
NheI 2149 GCTAGC G^CTAGC
4567 GCTAGC
NspI 2744 ACATGT [GA]CATG^[CT]
6809 GCATGT
PflFI 395 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
455 GACACAGTC
PflMI 4203 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4228 CCAGCCCCTGG
SapI 5761 GAAGAGC GCTCTTC(1/4)
2418 GCTCTTC
SfoI 1482 GGCGCC GGC^GCC
7011 GGCGCC
SmaI 2 CCCGGG CCC^GGG
1049 CCCGGG
Tth111I 395 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
455 GACACAGTC
Van91I 4203 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4228 CCAGCCCCTGG
XmaCI 2 CCCGGG C^CCGGG
1049 CCCGGG
XmaI 2 CCCGGG C^CCGGG
1049 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoRV EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NsiI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XcmI XhoII XmnI