##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AviII BalI
BbeI BbrPI BfrI BglI BseAI
BsiWI BsmBI BspDI BspEI BsrBI
BssHII Bst1107I BstUI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FseI FspI HaeII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI PacI PinAI PmaCI
PmlI PshBI Psp1406I RsrII SacI
SacII SbfI SexAI SfiI SfoI
SgrAI SpeI SphI Sse8387I StuI
SwaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1129 GACGTC GACGT^C
ApaI 891 GGGCCC GGGCC^C
AspI 2764 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1661 CGAAGTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5294 GGATAC GTATCC(6/5)
BclI 4507 TGATCA T^GATCA
BsaBI 4502 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 769 TCCGGT [AT]^CCGG[AT]
BsiEI 418 CGATCG CG[GA][CT]^CG
BspLU11I 164 ACATGT A^CATGT
BsrFI 850 GCCGGT [GA]^CCGG[CT]
BstAPI 3103 GCAAAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1848 GGTCACC G^GT[ACGT]ACC
BstPI 1848 GGTCACC G^GT[ACGT]ACC
CfoI 2878 GCGC GCG^C
Cfr10I 850 GCCGGT [GA]^CCGG[CT]
DrdI 1362 GACATGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1848 GGTCACC G^GT[ACGT]ACC
EcoT22I 1468 ATGCAT ATGCA^T
FbaI 4507 TGATCA T^GATCA
HhaI 2878 GCGC GCG^C
HinP1I 2878 GCGC G^CGC
MamI 4502 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 1468 ATGCAT ATGCA^T
PaeR7I 1011 CTCGAG C^TCGAG
PflFI 2764 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 5016 GTTTAAAC GTTT^AAAC
PpuMI 380 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 81 GACGACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 418 CGATCG CGAT^CG
SalI 88 GTCGAC G^TCGAC
ScaI 4458 AGTACT AGT^ACT
SmaI 1135 CCCGGG CCC^GGG
SnaBI 2848 TACGTA TAC^GTA
SspI 4902 AATATT AAT^ATT
Tth111I 2764 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4897 TCTAGA T^CTAGA
XhoI 1011 CTCGAG C^TCGAG
XmaCI 1135 CCCGGG C^CCGGG
XmaI 1135 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccI 88 GTCGAC GT^[AC][GT]AC
2770 GTCTAC
AcyI 1129 GACGTC G[GA]^CG[CT]C
1368 GGCGTC
Alw44I 2588 GTGCAC G^TGCAC
4359 GTGCAC
AocI 2778 CCTCAGG CC^T[ACGT]AGG
3818 CCTCAGG
ApaLI 2588 GTGCAC G^TGCAC
4359 GTGCAC
AvrII 3262 CCTAGG C^CTAGG
4636 CCTAGG
BamHI 1529 GGATCC G^GATCC
2169 GGATCC
BbvCI 3872 CCTCAGC CCTCAGC(-5/-2)
3112 GCTGAGG
BlnI 3262 CCTAGG C^CTAGG
4636 CCTAGG
BlpI 2128 GCTAAGC GC^T[ACGT]AGC
4176 GCTTAGC
Bpu1102I 2128 GCTAAGC GC^T[ACGT]AGC
4176 GCTTAGC
BsaAI 1244 CACGTA [CT]AC^GT[GA]
2848 TACGTA
BsaHI 1129 GACGTC G[GA]^CG[CT]C
1368 GGCGTC
BseRI 3131 CTCCTC GAGGAG(10/8)
1597 GAGGAG
BsmI 2519 GAATGC GAATGC(1/-1)
4758 GCATTC
BspHI 3207 TCATGA T^CATGA
3780 TCATGA
BsrDI 4215 CATTGC GCAATG(2/0)
4867 GCAATG
BssSI 2608 CACGAG CACGAG(-5/-1)
3899 CACGAG
BstBI 264 TTCGAA TT^CGAA
5242 TTCGAA
Bsu36I 2778 CCTCAGG CC^T[ACGT]AGG
3818 CCTCAGG
CelII 2128 GCTAAGC GC^T[ACGT]AGC
4176 GCTTAGC
DraII 380 GGGACCC [GA]G^G[ACGT]CC[CT]
3476 AGGCCCC
DraIII 1019 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4353 CACTGCGTG
EaeI 848 TGGCCG [CT]^GGCC[GA]
939 TGGCCG
Eco81I 2778 CCTCAGG CC^T[ACGT]AGG
3818 CCTCAGG
EcoO109I 380 GGGACCC [GA]G^G[ACGT]CC[CT]
3476 AGGCCCC
HgaI 1369 GCGTC GACGC(5/10)
3192 GCGTC
Hin1I 1129 GACGTC G[GA]^CG[CT]C
1368 GGCGTC
MspA1I 662 CAGCTG C[AC]G^C[GT]G
3947 CAGCTG
NcoI 4739 CCATGG C^CATGG
4861 CCATGG
NdeI 4401 CATATG CA^TATG
4913 CATATG
NspI 164 ACATGT [GA]CATG^[CT]
5272 ACATGC
NspV 264 TTCGAA TT^CGAA
5242 TTCGAA
PflMI 1358 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1717 CCACAAAATGG
PvuII 662 CAGCTG CAG^CTG
3947 CAGCTG
RcaI 3207 TCATGA T^CATGA
3780 TCATGA
SapI 873 GAAGAGC GCTCTTC(1/4)
992 GCTCTTC
SfuI 264 TTCGAA TT^CGAA
5242 TTCGAA
SmlI 1011 CTCGAG C^T[CT][GA]AG
5469 CTTGAG
Van91I 1358 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1717 CCACAAAATGG
XcmI 273 CCACTCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2502 CCAAAATGATTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DsaI EarI EcoNI EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI