################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII Acc65I AccIII Aor51HI ApaI AscI Asp718I AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BlnI BsaBI BseAI BsiWI BsmBI BspEI BspHI BssHII BstBI CpoI DraIII Eco47III EcoNI FspI HpaI KasI KpnI MamI MluI MroI NarI NotI NruI NspV PacI PflFI PmaCI PmeI PmlI PshAI PvuI RcaI RsrII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I StuI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AclI 2865 AACGTT AA^CGTT AflII 3871 CTTAAG C^TTAAG AgeI 359 ACCGGT A^CCGGT AhdI 2474 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 266 CCTGAGG CC^T[ACGT]AGG AspEI 2474 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 521 TGGCCA TGG^CCA BanI 323 GGCACC G^G[CT][GA]CC BanII 305 GAGCTC G[GA]GC[CT]^C BciVI 3374 GGATAC GTATCC(6/5) BclI 4671 TGATCA T^GATCA BfrI 3871 CTTAAG C^TTAAG BglII 500 AGATCT A^GATCT BsaAI 5199 CACGTA [CT]AC^GT[GA] BsaWI 359 ACCGGT [AT]^CCGG[AT] BspDI 2067 ATCGAT AT^CGAT BspMI 1085 ACCTGC ACCTGC(4/8) BstXI 671 CCATTATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 266 CCTGAGG CC^T[ACGT]AGG ClaI 2067 ATCGAT AT^CGAT DraII 108 GGGACCT [GA]G^G[ACGT]CC[CT] Eam1105I 2474 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 266 CCTGAGG CC^T[ACGT]AGG EcoO109I 108 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 3183 GATATC GAT^ATC FbaI 4671 TGATCA T^GATCA HaeII 2485 AGCGCC [GA]GCGC^[CT] HincII 549 GTTGAC GT[CT]^[GA]AC HindII 549 GTTGAC GT[CT]^[GA]AC MfeI 847 CAATTG C^AATTG MluNI 521 TGGCCA TGG^CCA MscI 521 TGGCCA TGG^CCA MunI 847 CAATTG C^AATTG NcoI 340 CCATGG C^CATGG NheI 5250 GCTAGC G^CTAGC PflMI 1488 CCAACCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 359 ACCGGT A^CCGGT PpuMI 108 GGGACCT [GA]G^G[AT]CC[CT] Psp1406I 2865 AACGTT AA^CGTT SacI 305 GAGCTC GAGCT^C SapI 3830 GAAGAGC GCTCTTC(1/4) ScaI 1972 AGTACT AGT^ACT SmaI 62 CCCGGG CCC^GGG SpeI 5262 ACTAGT A^CTAGT Van91I 1488 CCAACCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 62 CCCGGG C^CCGGG XmaI 62 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 4215 GTATAC GT^[AC][GT]AC 5202 GTATAC AcyI 78 GACGCC G[GA]^CG[CT]C 125 GGCGTC AflIII 1639 ACATGT A^C[GA][CT]GT 3284 ACATGT Alw44I 562 GTGCAC G^TGCAC 3294 GTGCAC AlwNI 528 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 2547 CAGACTCTG ApaLI 562 GTGCAC G^TGCAC 3294 GTGCAC AseI 2013 ATTAAT AT^TAAT 4925 ATTAAT AsnI 2013 ATTAAT AT^TAAT 4925 ATTAAT Asp700I 981 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1876 GAAGAATTTC BglI 96 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 514 GCCGTATTGGC BlpI 67 GCTGAGC GC^T[ACGT]AGC 3485 GCTCAGC Bpu1102I 67 GCTGAGC GC^T[ACGT]AGC 3485 GCTCAGC BsaHI 78 GACGCC G[GA]^CG[CT]C 125 GGCGTC BsgI 3042 CTGCAC GTGCAG(16/14) 3681 GTGCAG BsiEI 149 CGGCCG CG[GA][CT]^CG 247 CGGCCG BspLU11I 1639 ACATGT A^CATGT 3284 ACATGT BssSI 2418 CACGAG CACGAG(-5/-1) 4185 CTCGTG Bst1107I 4215 GTATAC GTA^TAC 5202 GTATAC BstEII 772 GGTCACC G^GT[ACGT]ACC 3310 GGTCACC BstPI 772 GGTCACC G^GT[ACGT]ACC 3310 GGTCACC BstZ17I 4215 GTATAC GTA^TAC 5202 GTATAC CelII 67 GCTGAGC GC^T[ACGT]AGC 3485 GCTCAGC EagI 149 CGGCCG C^GGCCG 247 CGGCCG EclXI 149 CGGCCG C^GGCCG 247 CGGCCG Eco52I 149 CGGCCG C^GGCCG 247 CGGCCG EcoO65I 772 GGTCACC G^GT[ACGT]ACC 3310 GGTCACC EcoRI 3055 GAATTC G^AATTC 3141 GAATTC FseI 146 GGCCGGCC GGCCGG^CC 248 GGCCGGCC Hin1I 78 GACGCC G[GA]^CG[CT]C 125 GGCGTC HindIII 2129 AAGCTT A^AGCTT 3955 AAGCTT KspI 73 CCGCGG CCGC^GG 234 CCGCGG PshBI 2013 ATTAAT AT^TAAT 4925 ATTAAT SacII 73 CCGCGG CCGC^GG 234 CCGCGG SspI 4747 AATATT AAT^ATT 4958 AATATT XmnI 981 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1876 GAAGAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrBI BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DrdI DsaI EaeI EarI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeI NdeII NgoMIV NlaIII NlaIV NsiI NspI PaeR7I PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoI XhoII