##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccIII Aor51HI ApaI
AscI Asp718I AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BlnI BsaBI BseAI BsiWI BsmBI
BspEI BspHI BssHII BstBI CpoI
DraIII Eco47III EcoNI FspI HpaI
KasI KpnI MamI MluI MroI
NarI NotI NruI NspV PacI
PflFI PmaCI PmeI PmlI PshAI
PvuI RcaI RsrII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I StuI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AclI 2865 AACGTT AA^CGTT
AflII 3871 CTTAAG C^TTAAG
AgeI 359 ACCGGT A^CCGGT
AhdI 2474 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 266 CCTGAGG CC^T[ACGT]AGG
AspEI 2474 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 521 TGGCCA TGG^CCA
BanI 323 GGCACC G^G[CT][GA]CC
BanII 305 GAGCTC G[GA]GC[CT]^C
BciVI 3374 GGATAC GTATCC(6/5)
BclI 4671 TGATCA T^GATCA
BfrI 3871 CTTAAG C^TTAAG
BglII 500 AGATCT A^GATCT
BsaAI 5199 CACGTA [CT]AC^GT[GA]
BsaWI 359 ACCGGT [AT]^CCGG[AT]
BspDI 2067 ATCGAT AT^CGAT
BspMI 1085 ACCTGC ACCTGC(4/8)
BstXI 671 CCATTATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 266 CCTGAGG CC^T[ACGT]AGG
ClaI 2067 ATCGAT AT^CGAT
DraII 108 GGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 2474 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 266 CCTGAGG CC^T[ACGT]AGG
EcoO109I 108 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 3183 GATATC GAT^ATC
FbaI 4671 TGATCA T^GATCA
HaeII 2485 AGCGCC [GA]GCGC^[CT]
HincII 549 GTTGAC GT[CT]^[GA]AC
HindII 549 GTTGAC GT[CT]^[GA]AC
MfeI 847 CAATTG C^AATTG
MluNI 521 TGGCCA TGG^CCA
MscI 521 TGGCCA TGG^CCA
MunI 847 CAATTG C^AATTG
NcoI 340 CCATGG C^CATGG
NheI 5250 GCTAGC G^CTAGC
PflMI 1488 CCAACCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 359 ACCGGT A^CCGGT
PpuMI 108 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2865 AACGTT AA^CGTT
SacI 305 GAGCTC GAGCT^C
SapI 3830 GAAGAGC GCTCTTC(1/4)
ScaI 1972 AGTACT AGT^ACT
SmaI 62 CCCGGG CCC^GGG
SpeI 5262 ACTAGT A^CTAGT
Van91I 1488 CCAACCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 62 CCCGGG C^CCGGG
XmaI 62 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 4215 GTATAC GT^[AC][GT]AC
5202 GTATAC
AcyI 78 GACGCC G[GA]^CG[CT]C
125 GGCGTC
AflIII 1639 ACATGT A^C[GA][CT]GT
3284 ACATGT
Alw44I 562 GTGCAC G^TGCAC
3294 GTGCAC
AlwNI 528 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2547 CAGACTCTG
ApaLI 562 GTGCAC G^TGCAC
3294 GTGCAC
AseI 2013 ATTAAT AT^TAAT
4925 ATTAAT
AsnI 2013 ATTAAT AT^TAAT
4925 ATTAAT
Asp700I 981 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1876 GAAGAATTTC
BglI 96 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
514 GCCGTATTGGC
BlpI 67 GCTGAGC GC^T[ACGT]AGC
3485 GCTCAGC
Bpu1102I 67 GCTGAGC GC^T[ACGT]AGC
3485 GCTCAGC
BsaHI 78 GACGCC G[GA]^CG[CT]C
125 GGCGTC
BsgI 3042 CTGCAC GTGCAG(16/14)
3681 GTGCAG
BsiEI 149 CGGCCG CG[GA][CT]^CG
247 CGGCCG
BspLU11I 1639 ACATGT A^CATGT
3284 ACATGT
BssSI 2418 CACGAG CACGAG(-5/-1)
4185 CTCGTG
Bst1107I 4215 GTATAC GTA^TAC
5202 GTATAC
BstEII 772 GGTCACC G^GT[ACGT]ACC
3310 GGTCACC
BstPI 772 GGTCACC G^GT[ACGT]ACC
3310 GGTCACC
BstZ17I 4215 GTATAC GTA^TAC
5202 GTATAC
CelII 67 GCTGAGC GC^T[ACGT]AGC
3485 GCTCAGC
EagI 149 CGGCCG C^GGCCG
247 CGGCCG
EclXI 149 CGGCCG C^GGCCG
247 CGGCCG
Eco52I 149 CGGCCG C^GGCCG
247 CGGCCG
EcoO65I 772 GGTCACC G^GT[ACGT]ACC
3310 GGTCACC
EcoRI 3055 GAATTC G^AATTC
3141 GAATTC
FseI 146 GGCCGGCC GGCCGG^CC
248 GGCCGGCC
Hin1I 78 GACGCC G[GA]^CG[CT]C
125 GGCGTC
HindIII 2129 AAGCTT A^AGCTT
3955 AAGCTT
KspI 73 CCGCGG CCGC^GG
234 CCGCGG
PshBI 2013 ATTAAT AT^TAAT
4925 ATTAAT
SacII 73 CCGCGG CCGC^GG
234 CCGCGG
SspI 4747 AATATT AAT^ATT
4958 AATATT
XmnI 981 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1876 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DrdI DsaI EaeI
EarI EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeI NdeII
NgoMIV NlaIII NlaIV NsiI NspI
PaeR7I PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoI XhoII