##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AviII
BcgI BclI BsaBI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoRI FbaI
FseI FspI HindIII HpaI KpnI
KspI MamI MluI MroI NaeI
NdeI NgoMIV NotI NruI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SfuI SgrAI SnaBI SpeI
SphI SspBI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 1327 CGCG CG^CG
AflII 4441 CTTAAG C^TTAAG
Aor51HI 3281 AGCGCT AGC^GCT
AspI 3342 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3945 CACGTG CAC^GTG
BfrI 4441 CTTAAG C^TTAAG
BglI 3778 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3945 CACGTG [CT]AC^GT[GA]
BsaWI 2724 TCCGGT [AT]^CCGG[AT]
BsrFI 3550 ACCGGC [GA]^CCGG[CT]
BssSI 2479 CACGAG CACGAG(-5/-1)
BstUI 1327 CGCG CG^CG
Cfr10I 3550 ACCGGC [GA]^CCGG[CT]
DraI 4460 TTTAAA TTT^AAA
DraIII 3942 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4241 GACATCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3281 AGCGCT AGC^GCT
EcoRV 586 GATATC GAT^ATC
EcoT22I 1364 ATGCAT ATGCA^T
HincII 3891 GTTGAC GT[CT]^[GA]AC
HindII 3891 GTTGAC GT[CT]^[GA]AC
MfeI 2103 CAATTG C^AATTG
MunI 2103 CAATTG C^AATTG
MvnI 1327 CGCG CG^CG
NsiI 1364 ATGCAT ATGCA^T
PflFI 3342 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3945 CACGTG CAC^GTG
PmlI 3945 CACGTG CAC^GTG
PshAI 2029 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 488 GCTCTTC GCTCTTC(1/4)
SbfI 3884 CCTGCAGG CCTGCA^GG
SfiI 3777 GGCCTCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 634 CCCGGG CCC^GGG
Sse8387I 3884 CCTGCAGG CCTGCA^GG
SspI 4612 AATATT AAT^ATT
Tth111I 3342 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2330 TCTAGA T^CTAGA
XcmI 2717 CCATGTCTCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 634 CCCGGG C^CCGGG
XmaI 634 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 2019 GAATTT [GA]^AATT[CT]
4206 AAATTC
AhdI 769 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3867 GACCTGCAGTC
ApoI 2019 GAATTT [GA]^AATT[CT]
4206 AAATTC
AspEI 769 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3867 GACCTGCAGTC
BalI 1674 TGGCCA TGG^CCA
4470 TGGCCA
BamHI 402 GGATCC G^GATCC
3586 GGATCC
BbeI 46 GGCGCC GGCGC^C
1831 GGCGCC
BbsI 1916 GAAGAC GAAGAC(2/6)
4404 GTCTTC
BglII 410 AGATCT A^GATCT
2954 AGATCT
BpuAI 1916 GAAGAC GAAGAC(2/6)
4404 GTCTTC
BsgI 629 CTGCAC GTGCAG(16/14)
3646 CTGCAC
BsmI 2175 GAATGC GAATGC(1/-1)
2586 GCATTC
BstAPI 1330 GCAGCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3438 GCAGCCCCTGC
BstXI 735 CCAGCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2160 CCAAGCCTATGG
Eam1105I 769 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3867 GACCTGCAGTC
KasI 46 GGCGCC G^GCGCC
1831 GGCGCC
MaeII 1016 ACGT A^CGT
3946 ACGT
MluNI 1674 TGGCCA TGG^CCA
4470 TGGCCA
MscI 1674 TGGCCA TGG^CCA
4470 TGGCCA
NarI 46 GGCGCC GG^CGCC
1831 GGCGCC
NheI 25 GCTAGC G^CTAGC
3327 GCTAGC
NspI 3382 ACATGC [GA]CATG^[CT]
4092 GCATGT
PaeR7I 2598 CTCGAG C^TCGAG
3772 CTCGAG
SacI 1241 GAGCTC GAGCT^C
1735 GAGCTC
SexAI 3178 ACCTGGT A^CC[AT]GGT
4504 ACCAGGT
SfoI 46 GGCGCC GGC^GCC
1831 GGCGCC
TaqI 2599 TCGA T^CGA
3773 TCGA
TthHB8I 2599 TCGA T^CGA
3773 TCGA
XhoI 2598 CTCGAG C^TCGAG
3772 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII AvrII BanI BanII BbvCI
BbvI BciVI BcnI BfaI BlnI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII