################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## AatII Acc65I AclI AscI AseI AsnI Asp718I BcgI BsaBI BsiWI BsmBI BspDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI EcoO65I EcoRV FseI KpnI MamI MfeI MluI MunI NdeI NheI NotI NruI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SfiI SfuI SnaBI SpeI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 4621 GTCTAC GT^[AC][GT]AC AccIII 1288 TCCGGA T^CCGGA AcyI 1541 GGCGCC G[GA]^CG[CT]C Aor51HI 1302 AGCGCT AGC^GCT AviII 1701 TGCGCA TGC^GCA AvrII 4937 CCTAGG C^CTAGG BbeI 1541 GGCGCC GGCGC^C BbrPI 995 CACGTG CAC^GTG BclI 2853 TGATCA T^GATCA BlnI 4937 CCTAGG C^CTAGG BlpI 2974 GCTTAGC GC^T[ACGT]AGC Bpu1102I 2974 GCTTAGC GC^T[ACGT]AGC BsaHI 1541 GGCGCC G[GA]^CG[CT]C BseAI 1288 TCCGGA T^CCGGA Bsp1407I 3825 TGTACA T^GTACA BspEI 1288 TCCGGA T^CCGGA BsrDI 2570 CATTGC GCAATG(2/0) BsrGI 3825 TGTACA T^GTACA BstXI 2624 CCAGGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2974 GCTTAGC GC^T[ACGT]AGC DraI 3176 TTTAAA TTT^AAA Eco47III 1302 AGCGCT AGC^GCT EcoRI 1844 GAATTC G^AATTC EcoT22I 5242 ATGCAT ATGCA^T FbaI 2853 TGATCA T^GATCA FspI 1701 TGCGCA TGC^GCA HgaI 4791 GCGTC GACGC(5/10) Hin1I 1541 GGCGCC G[GA]^CG[CT]C HpaI 3468 GTTAAC GTT^AAC KasI 1541 GGCGCC G^GCGCC KspI 1533 CCGCGG CCGC^GG MroI 1288 TCCGGA T^CCGGA NaeI 346 GCCGGC GCC^GGC NarI 1541 GGCGCC GG^CGCC NcoI 870 CCATGG C^CATGG NgoMIV 346 GCCGGC G^CCGGC NsiI 5242 ATGCAT ATGCA^T PmaCI 995 CACGTG CAC^GTG PmlI 995 CACGTG CAC^GTG PshAI 4470 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 470 GAGCTC GAGCT^C SacII 1533 CCGCGG CCGC^GG SexAI 1548 ACCAGGT A^CC[AT]GGT SfoI 1541 GGCGCC GGC^GCC SspBI 3825 TGTACA T^GTACA XbaI 3242 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccII 658 CGCG CG^CG 1534 CGCG AcsI 1844 GAATTC [GA]^AATT[CT] 3171 GAATTT AflII 152 CTTAAG C^TTAAG 4102 CTTAAG AgeI 3261 ACCGGT A^CCGGT 3407 ACCGGT ApaI 46 GGGCCC GGGCC^C 2152 GGGCCC ApoI 1844 GAATTC [GA]^AATT[CT] 3171 GAATTT AspI 3429 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5037 GACCCTGTC BamHI 489 GGATCC G^GATCC 4649 GGATCC BciVI 4899 GGATAC GTATCC(6/5) 2510 GTATCC BfrI 152 CTTAAG C^TTAAG 4102 CTTAAG BsaAI 995 CACGTG [CT]AC^GT[GA] 3812 CACGTA BspHI 3630 TCATGA T^CATGA 4403 TCATGA BspLU11I 651 ACATGT A^CATGT 3234 ACATGT BstUI 658 CGCG CG^CG 1534 CGCG EagI 800 CGGCCG C^GGCCG 1056 CGGCCG EclXI 800 CGGCCG C^GGCCG 1056 CGGCCG Eco52I 800 CGGCCG C^GGCCG 1056 CGGCCG HincII 2992 GTTGAC GT[CT]^[GA]AC 3468 GTTAAC HindII 2992 GTTGAC GT[CT]^[GA]AC 3468 GTTAAC HindIII 2000 AAGCTT A^AGCTT 3399 AAGCTT MvnI 658 CGCG CG^CG 1534 CGCG PflFI 3429 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5037 GACCCTGTC PflMI 70 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 106 CCAGTGCCTGG PinAI 3261 ACCGGT A^CCGGT 3407 ACCGGT RcaI 3630 TCATGA T^CATGA 4403 TCATGA SgrAI 905 CACCGGCG C[GA]^CCGG[CT]G 1334 CACCGGCG SmaI 1266 CCCGGG CCC^GGG 3782 CCCGGG SphI 19 GCATGC GCATG^C 1429 GCATGC TaqI 1047 TCGA T^CGA 3598 TCGA Tth111I 3429 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5037 GACCCTGTC TthHB8I 1047 TCGA T^CGA 3598 TCGA Van91I 70 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 106 CCAGTGCCTGG XmaCI 1266 CCCGGG C^CCGGG 3782 CCCGGG XmaI 1266 CCCGGG C^CCGGG 3782 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AciI AfaI AflIII AhdI AluI Alw44I AlwI AlwNI AocI ApaLI Asp700I AspEI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII XmnI