##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII Acc65I AclI AscI AseI
AsnI Asp718I BcgI BsaBI BsiWI
BsmBI BspDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI EcoO65I EcoRV FseI KpnI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PmeI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SfiI
SfuI SnaBI SpeI Sse8387I SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 4621 GTCTAC GT^[AC][GT]AC
AccIII 1288 TCCGGA T^CCGGA
AcyI 1541 GGCGCC G[GA]^CG[CT]C
Aor51HI 1302 AGCGCT AGC^GCT
AviII 1701 TGCGCA TGC^GCA
AvrII 4937 CCTAGG C^CTAGG
BbeI 1541 GGCGCC GGCGC^C
BbrPI 995 CACGTG CAC^GTG
BclI 2853 TGATCA T^GATCA
BlnI 4937 CCTAGG C^CTAGG
BlpI 2974 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2974 GCTTAGC GC^T[ACGT]AGC
BsaHI 1541 GGCGCC G[GA]^CG[CT]C
BseAI 1288 TCCGGA T^CCGGA
Bsp1407I 3825 TGTACA T^GTACA
BspEI 1288 TCCGGA T^CCGGA
BsrDI 2570 CATTGC GCAATG(2/0)
BsrGI 3825 TGTACA T^GTACA
BstXI 2624 CCAGGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2974 GCTTAGC GC^T[ACGT]AGC
DraI 3176 TTTAAA TTT^AAA
Eco47III 1302 AGCGCT AGC^GCT
EcoRI 1844 GAATTC G^AATTC
EcoT22I 5242 ATGCAT ATGCA^T
FbaI 2853 TGATCA T^GATCA
FspI 1701 TGCGCA TGC^GCA
HgaI 4791 GCGTC GACGC(5/10)
Hin1I 1541 GGCGCC G[GA]^CG[CT]C
HpaI 3468 GTTAAC GTT^AAC
KasI 1541 GGCGCC G^GCGCC
KspI 1533 CCGCGG CCGC^GG
MroI 1288 TCCGGA T^CCGGA
NaeI 346 GCCGGC GCC^GGC
NarI 1541 GGCGCC GG^CGCC
NcoI 870 CCATGG C^CATGG
NgoMIV 346 GCCGGC G^CCGGC
NsiI 5242 ATGCAT ATGCA^T
PmaCI 995 CACGTG CAC^GTG
PmlI 995 CACGTG CAC^GTG
PshAI 4470 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 470 GAGCTC GAGCT^C
SacII 1533 CCGCGG CCGC^GG
SexAI 1548 ACCAGGT A^CC[AT]GGT
SfoI 1541 GGCGCC GGC^GCC
SspBI 3825 TGTACA T^GTACA
XbaI 3242 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 658 CGCG CG^CG
1534 CGCG
AcsI 1844 GAATTC [GA]^AATT[CT]
3171 GAATTT
AflII 152 CTTAAG C^TTAAG
4102 CTTAAG
AgeI 3261 ACCGGT A^CCGGT
3407 ACCGGT
ApaI 46 GGGCCC GGGCC^C
2152 GGGCCC
ApoI 1844 GAATTC [GA]^AATT[CT]
3171 GAATTT
AspI 3429 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5037 GACCCTGTC
BamHI 489 GGATCC G^GATCC
4649 GGATCC
BciVI 4899 GGATAC GTATCC(6/5)
2510 GTATCC
BfrI 152 CTTAAG C^TTAAG
4102 CTTAAG
BsaAI 995 CACGTG [CT]AC^GT[GA]
3812 CACGTA
BspHI 3630 TCATGA T^CATGA
4403 TCATGA
BspLU11I 651 ACATGT A^CATGT
3234 ACATGT
BstUI 658 CGCG CG^CG
1534 CGCG
EagI 800 CGGCCG C^GGCCG
1056 CGGCCG
EclXI 800 CGGCCG C^GGCCG
1056 CGGCCG
Eco52I 800 CGGCCG C^GGCCG
1056 CGGCCG
HincII 2992 GTTGAC GT[CT]^[GA]AC
3468 GTTAAC
HindII 2992 GTTGAC GT[CT]^[GA]AC
3468 GTTAAC
HindIII 2000 AAGCTT A^AGCTT
3399 AAGCTT
MvnI 658 CGCG CG^CG
1534 CGCG
PflFI 3429 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5037 GACCCTGTC
PflMI 70 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
106 CCAGTGCCTGG
PinAI 3261 ACCGGT A^CCGGT
3407 ACCGGT
RcaI 3630 TCATGA T^CATGA
4403 TCATGA
SgrAI 905 CACCGGCG C[GA]^CCGG[CT]G
1334 CACCGGCG
SmaI 1266 CCCGGG CCC^GGG
3782 CCCGGG
SphI 19 GCATGC GCATG^C
1429 GCATGC
TaqI 1047 TCGA T^CGA
3598 TCGA
Tth111I 3429 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5037 GACCCTGTC
TthHB8I 1047 TCGA T^CGA
3598 TCGA
Van91I 70 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
106 CCAGTGCCTGG
XmaCI 1266 CCCGGG C^CCGGG
3782 CCCGGG
XmaI 1266 CCCGGG C^CCGGG
3782 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AfaI AflIII AhdI AluI
Alw44I AlwI AlwNI AocI ApaLI
Asp700I AspEI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII
XmnI