##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflII AgeI AhdI AlwNI ApaI
AscI Asp718I AspEI AspI AviII
AvrII BalI BbeI BbrPI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BseAI BsiEI BsiWI
BsmBI BspDI BspEI BsrFI BssHII
Bst1107I BstAPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco52I
EcoRV FbaI FseI FspI HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NarI NgoMIV NotI
NruI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SphI Sse8387I SspI
Tth111I Van91I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AclI 2651 AACGTT AA^CGTT
AcyI 3410 GGCGTC G[GA]^CG[CT]C
Alw44I 282 GTGCAC G^TGCAC
Aor51HI 1506 AGCGCT AGC^GCT
ApaLI 282 GTGCAC G^TGCAC
AseI 3860 ATTAAT AT^TAAT
AsnI 3860 ATTAAT AT^TAAT
BamHI 2036 GGATCC G^GATCC
BanI 1181 GGCACC G^G[CT][GA]CC
BbvCI 39 GCTGAGG CCTCAGC(-5/-2)
BpmI 1667 CTCCAG CTGGAG(16/14)
BsaAI 2764 TACGTA [CT]AC^GT[GA]
BsaBI 1490 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3410 GGCGTC G[GA]^CG[CT]C
BsgI 1008 GTGCAG GTGCAG(16/14)
BspHI 2195 TCATGA T^CATGA
BsrDI 3537 CATTGC GCAATG(2/0)
BstBI 1930 TTCGAA TT^CGAA
BstEII 202 GGTCACC G^GT[ACGT]ACC
BstPI 202 GGTCACC G^GT[ACGT]ACC
DsaI 2242 CCATGG C^C[GA][CT]GG
Eco47III 1506 AGCGCT AGC^GCT
EcoNI 2681 CCTTGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 202 GGTCACC G^GT[ACGT]ACC
EcoT22I 2551 ATGCAT ATGCA^T
Hin1I 3410 GGCGTC G[GA]^CG[CT]C
HincII 3700 GTCAAC GT[CT]^[GA]AC
HindII 3700 GTCAAC GT[CT]^[GA]AC
HindIII 3342 AAGCTT A^AGCTT
MamI 1490 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 3227 CAGCTG C[AC]G^C[GT]G
NcoI 2242 CCATGG C^CATGG
NdeI 4709 CATATG CA^TATG
NsiI 2551 ATGCAT ATGCA^T
NspV 1930 TTCGAA TT^CGAA
PaeR7I 1819 CTCGAG C^TCGAG
PshAI 7 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3860 ATTAAT AT^TAAT
Psp1406I 2651 AACGTT AA^CGTT
PstI 1399 CTGCAG CTGCA^G
PvuII 3227 CAGCTG CAG^CTG
RcaI 2195 TCATGA T^CATGA
SacI 1047 GAGCTC GAGCT^C
SapI 1948 GAAGAGC GCTCTTC(1/4)
ScaI 2065 AGTACT AGT^ACT
SexAI 3079 ACCTGGT A^CC[AT]GGT
SfuI 1930 TTCGAA TT^CGAA
SnaBI 2764 TACGTA TAC^GTA
SpeI 2819 ACTAGT A^CTAGT
StuI 936 AGGCCT AGG^CCT
SwaI 3864 ATTTAAAT ATTT^AAAT
XhoI 1819 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 3704 ACATGT A^C[GA][CT]GT
4325 ACATGT
AocI 1311 CCTCAGG CC^T[ACGT]AGG
3729 CCTCAGG
AspHI 282 GTGCAC G[AT]GC[AT]^C
1047 GAGCTC
AvaI 1473 CCCGAG C^[CT]CG[GA]G
1819 CTCGAG
BciVI 98 GGATAC GTATCC(6/5)
1356 GTATCC
BsaWI 811 ACCGGA [AT]^CCGG[AT]
1016 ACCGGA
BsiHKAI 282 GTGCAC G[AT]GC[AT]^C
1047 GAGCTC
BsoBI 1473 CCCGAG C^[CT]CG[GA]G
1819 CTCGAG
Bsp1407I 2726 TGTACA T^GTACA
3368 TGTACA
BspLU11I 3704 ACATGT A^CATGT
4325 ACATGT
BsrGI 2726 TGTACA T^GTACA
3368 TGTACA
BstXI 2207 CCAAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3073 CCATAAACCTGG
Bsu36I 1311 CCTCAGG CC^T[ACGT]AGG
3729 CCTCAGG
CfoI 1507 GCGC GCG^C
2153 GCGC
DraII 830 AGGACCC [GA]G^G[ACGT]CC[CT]
4691 GGGACCC
Eco81I 1311 CCTCAGG CC^T[ACGT]AGG
3729 CCTCAGG
EcoO109I 830 AGGACCC [GA]G^G[ACGT]CC[CT]
4691 GGGACCC
EcoRI 1278 GAATTC G^AATTC
2961 GAATTC
FauI 1649 GCGGG CCCGC(4/6)
1766 GCGGG
HaeII 1506 AGCGCT [GA]GCGC^[CT]
2152 GGCGCT
HhaI 1507 GCGC GCG^C
2153 GCGC
HinP1I 1507 GCGC G^CGC
2153 GCGC
PpuMI 830 AGGACCC [GA]G^G[AT]CC[CT]
4691 GGGACCC
SspBI 2726 TGTACA T^GTACA
3368 TGTACA
XbaI 561 TCTAGA T^CTAGA
668 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrBI BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NheI NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI