##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccIII AclI AgeI AscI
Asp718I AvaI AvrII BbeI BbrPI
BcgI BglI BlnI BsaI BsaWI
BseAI BsiWI BsoBI BspEI BspHI
BsrFI BssHII Bst1107I BstEII BstPI
BstXI BstZ17I Cfr10I CpoI EcoNI
EcoO65I EcoRI EcoT22I FseI HindIII
KasI KpnI KspI MroI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SexAI SfiI SfoI SgrAI SmaI
SpeI SphI SspI StuI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 3157 GACGTC GACGT^C
AccI 1933 GTCTAC GT^[AC][GT]AC
AflII 3922 CTTAAG C^TTAAG
AhdI 279 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 326 AGCGCT AGC^GCT
ApaI 72 GGGCCC GGGCC^C
AspEI 279 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1174 TGCGCA TGC^GCA
BamHI 4374 GGATCC G^GATCC
BanI 4122 GGTGCC G^G[CT][GA]CC
BciVI 197 GTATCC GTATCC(6/5)
BclI 2224 TGATCA T^GATCA
BfrI 3922 CTTAAG C^TTAAG
BsaAI 2834 TACGTA [CT]AC^GT[GA]
BsaBI 696 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2051 CGGCCG CG[GA][CT]^CG
BspLU11I 1226 ACATGT A^CATGT
BspMI 361 ACCTGC ACCTGC(4/8)
BsrBI 2054 CCGCTC CCGCTC(-3/-3)
BsrDI 4771 GCAATG GCAATG(2/0)
BssSI 4828 CTCGTG CACGAG(-5/-1)
DrdI 2781 GACGTTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2051 CGGCCG C^GGCCG
Eam1105I 279 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2051 CGGCCG C^GGCCG
Eco47III 326 AGCGCT AGC^GCT
Eco52I 2051 CGGCCG C^GGCCG
EcoRV 2916 GATATC GAT^ATC
FbaI 2224 TGATCA T^GATCA
FspI 1174 TGCGCA TGC^GCA
HpaI 3927 GTTAAC GTT^AAC
MamI 696 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3700 CAATTG C^AATTG
MunI 3700 CAATTG C^AATTG
PflMI 971 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 2154 CCTGCAGG CCTGCA^GG
ScaI 1354 AGTACT AGT^ACT
SnaBI 2834 TACGTA TAC^GTA
Sse8387I 2154 CCTGCAGG CCTGCA^GG
Van91I 971 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 4169 CCATCTCTATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 1962 GTGCAC G^TGCAC
4028 GTGCAC
AocI 3477 CCTGAGG CC^T[ACGT]AGG
4780 CCTCAGG
ApaLI 1962 GTGCAC G^TGCAC
4028 GTGCAC
AseI 2904 ATTAAT AT^TAAT
3635 ATTAAT
AsnI 2904 ATTAAT AT^TAAT
3635 ATTAAT
AspI 2752 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4549 GACGATGTC
BglII 265 AGATCT A^GATCT
4982 AGATCT
BsmI 1235 GCATTC GAATGC(1/-1)
4443 GCATTC
BspDI 1125 ATCGAT AT^CGAT
4894 ATCGAT
BstAPI 1166 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1177 GCATGGCCTGC
BstBI 1037 TTCGAA TT^CGAA
4559 TTCGAA
Bsu36I 3477 CCTGAGG CC^T[ACGT]AGG
4780 CCTCAGG
ClaI 1125 ATCGAT AT^CGAT
4894 ATCGAT
Eco81I 3477 CCTGAGG CC^T[ACGT]AGG
4780 CCTCAGG
HincII 276 GTTGAC GT[CT]^[GA]AC
3927 GTTAAC
HindII 276 GTTGAC GT[CT]^[GA]AC
3927 GTTAAC
MluI 3191 ACGCGT A^CGCGT
4256 ACGCGT
NheI 2084 GCTAGC G^CTAGC
2726 GCTAGC
NspV 1037 TTCGAA TT^CGAA
4559 TTCGAA
PflFI 2752 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4549 GACGATGTC
PshBI 2904 ATTAAT AT^TAAT
3635 ATTAAT
SacI 1728 GAGCTC GAGCT^C
4846 GAGCTC
SfuI 1037 TTCGAA TT^CGAA
4559 TTCGAA
Tth111I 2752 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4549 GACGATGTC
XbaI 851 TCTAGA T^CTAGA
3622 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BalI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI