################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII BbrPI BfrI BsaAI BseAI BsiEI BsiWI BsmBI BspDI BspEI BssHII Bst1107I BstAPI BstUI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI FspI HpaI KpnI KspI MluI MroI MvnI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI ScaI SfiI SgrAI SnaBI Tth111I XcmI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflIII 2546 ACATGT A^C[GA][CT]GT Alw44I 2066 GTGCAC G^TGCAC AocI 2774 CCTGAGG CC^T[ACGT]AGG ApaI 1295 GGGCCC GGGCC^C ApaLI 2066 GTGCAC G^TGCAC BbeI 1985 GGCGCC GGCGC^C BcgI 3789 GCATGTTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1556 GTATCC GTATCC(6/5) BclI 4229 TGATCA T^GATCA BsmI 2363 GAATGC GAATGC(1/-1) BspLU11I 2546 ACATGT A^CATGT BsrBI 78 CCGCTC CCGCTC(-3/-3) BsrDI 281 CATTGC GCAATG(2/0) BssSI 1594 CTCGTG CACGAG(-5/-1) BstEII 885 GGTCACC G^GT[ACGT]ACC BstPI 885 GGTCACC G^GT[ACGT]ACC BstXI 1133 CCAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2774 CCTGAGG CC^T[ACGT]AGG DraIII 3111 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 2774 CCTGAGG CC^T[ACGT]AGG EcoO65I 885 GGTCACC G^GT[ACGT]ACC EcoRI 3341 GAATTC G^AATTC FbaI 4229 TGATCA T^GATCA HincII 3250 GTCAAC GT[CT]^[GA]AC HindII 3250 GTCAAC GT[CT]^[GA]AC KasI 1985 GGCGCC G^GCGCC MfeI 4568 CAATTG C^AATTG MunI 4568 CAATTG C^AATTG NaeI 466 GCCGGC GCC^GGC NarI 1985 GGCGCC GG^CGCC NdeI 3127 CATATG CA^TATG NgoMIV 466 GCCGGC G^CCGGC NheI 1903 GCTAGC G^CTAGC PmeI 2413 GTTTAAAC GTTT^AAAC SacI 4111 GAGCTC GAGCT^C SfoI 1985 GGCGCC GGC^GCC SmaI 4482 CCCGGG CCC^GGG SpeI 4445 ACTAGT A^CTAGT SphI 4437 GCATGC GCATG^C XbaI 3146 TCTAGA T^CTAGA XmaCI 4482 CCCGGG C^CCGGG XmaI 4482 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 637 GTCTAC GT^[AC][GT]AC 1333 GTAGAC Asp700I 324 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3876 GAATCTGTTC AvrII 1946 CCTAGG C^CTAGG 4468 CCTAGG BamHI 250 GGATCC G^GATCC 4478 GGATCC BanI 621 GGTGCC G^G[CT][GA]CC 1985 GGCGCC BglII 362 AGATCT A^GATCT 2840 AGATCT BlnI 1946 CCTAGG C^CTAGG 4468 CCTAGG BsaBI 262 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 357 GATCCAGATC BsaWI 898 TCCGGT [AT]^CCGG[AT] 1500 TCCGGT BspHI 137 TCATGA T^CATGA 1505 TCATGA BsrFI 466 GCCGGC [GA]^CCGG[CT] 1643 ACCGGC BstBI 798 TTCGAA TT^CGAA 2309 TTCGAA Cfr10I 466 GCCGGC [GA]^CCGG[CT] 1643 ACCGGC EcoNI 1959 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3313 CCTGAAGAAGG EcoT22I 2050 ATGCAT ATGCA^T 2523 ATGCAT FauI 772 GCGGG CCCGC(4/6) 3327 GCGGG HaeII 1938 AGCGCC [GA]GCGC^[CT] 1985 GGCGCC HgaI 400 GACGC GACGC(5/10) 596 GCGTC HindIII 2220 AAGCTT A^AGCTT 3449 AAGCTT MamI 262 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 357 GATCCAGATC NcoI 2864 CCATGG C^CATGG 3885 CCATGG NsiI 2050 ATGCAT ATGCA^T 2523 ATGCAT NspV 798 TTCGAA TT^CGAA 2309 TTCGAA PpuMI 2968 AGGTCCT [GA]G^G[AT]CC[CT] 3440 AGGACCT RcaI 137 TCATGA T^CATGA 1505 TCATGA SbfI 851 CCTGCAGG CCTGCA^GG 1059 CCTGCAGG SexAI 780 ACCAGGT A^CC[AT]GGT 3571 ACCAGGT SfuI 798 TTCGAA TT^CGAA 2309 TTCGAA Sse8387I 851 CCTGCAGG CCTGCA^GG 1059 CCTGCAGG SspI 3004 AATATT AAT^ATT 3365 AATATT SwaI 1448 ATTTAAAT ATTT^AAAT 2470 ATTTAAAT XmnI 324 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3876 GAATCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII