##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AviII BbrPI BfrI BsaAI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BssHII Bst1107I BstAPI BstUI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FseI
FspI HpaI KpnI KspI MluI
MroI MvnI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI ScaI SfiI
SgrAI SnaBI Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflIII 2546 ACATGT A^C[GA][CT]GT
Alw44I 2066 GTGCAC G^TGCAC
AocI 2774 CCTGAGG CC^T[ACGT]AGG
ApaI 1295 GGGCCC GGGCC^C
ApaLI 2066 GTGCAC G^TGCAC
BbeI 1985 GGCGCC GGCGC^C
BcgI 3789 GCATGTTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1556 GTATCC GTATCC(6/5)
BclI 4229 TGATCA T^GATCA
BsmI 2363 GAATGC GAATGC(1/-1)
BspLU11I 2546 ACATGT A^CATGT
BsrBI 78 CCGCTC CCGCTC(-3/-3)
BsrDI 281 CATTGC GCAATG(2/0)
BssSI 1594 CTCGTG CACGAG(-5/-1)
BstEII 885 GGTCACC G^GT[ACGT]ACC
BstPI 885 GGTCACC G^GT[ACGT]ACC
BstXI 1133 CCAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2774 CCTGAGG CC^T[ACGT]AGG
DraIII 3111 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2774 CCTGAGG CC^T[ACGT]AGG
EcoO65I 885 GGTCACC G^GT[ACGT]ACC
EcoRI 3341 GAATTC G^AATTC
FbaI 4229 TGATCA T^GATCA
HincII 3250 GTCAAC GT[CT]^[GA]AC
HindII 3250 GTCAAC GT[CT]^[GA]AC
KasI 1985 GGCGCC G^GCGCC
MfeI 4568 CAATTG C^AATTG
MunI 4568 CAATTG C^AATTG
NaeI 466 GCCGGC GCC^GGC
NarI 1985 GGCGCC GG^CGCC
NdeI 3127 CATATG CA^TATG
NgoMIV 466 GCCGGC G^CCGGC
NheI 1903 GCTAGC G^CTAGC
PmeI 2413 GTTTAAAC GTTT^AAAC
SacI 4111 GAGCTC GAGCT^C
SfoI 1985 GGCGCC GGC^GCC
SmaI 4482 CCCGGG CCC^GGG
SpeI 4445 ACTAGT A^CTAGT
SphI 4437 GCATGC GCATG^C
XbaI 3146 TCTAGA T^CTAGA
XmaCI 4482 CCCGGG C^CCGGG
XmaI 4482 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 637 GTCTAC GT^[AC][GT]AC
1333 GTAGAC
Asp700I 324 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3876 GAATCTGTTC
AvrII 1946 CCTAGG C^CTAGG
4468 CCTAGG
BamHI 250 GGATCC G^GATCC
4478 GGATCC
BanI 621 GGTGCC G^G[CT][GA]CC
1985 GGCGCC
BglII 362 AGATCT A^GATCT
2840 AGATCT
BlnI 1946 CCTAGG C^CTAGG
4468 CCTAGG
BsaBI 262 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
357 GATCCAGATC
BsaWI 898 TCCGGT [AT]^CCGG[AT]
1500 TCCGGT
BspHI 137 TCATGA T^CATGA
1505 TCATGA
BsrFI 466 GCCGGC [GA]^CCGG[CT]
1643 ACCGGC
BstBI 798 TTCGAA TT^CGAA
2309 TTCGAA
Cfr10I 466 GCCGGC [GA]^CCGG[CT]
1643 ACCGGC
EcoNI 1959 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3313 CCTGAAGAAGG
EcoT22I 2050 ATGCAT ATGCA^T
2523 ATGCAT
FauI 772 GCGGG CCCGC(4/6)
3327 GCGGG
HaeII 1938 AGCGCC [GA]GCGC^[CT]
1985 GGCGCC
HgaI 400 GACGC GACGC(5/10)
596 GCGTC
HindIII 2220 AAGCTT A^AGCTT
3449 AAGCTT
MamI 262 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
357 GATCCAGATC
NcoI 2864 CCATGG C^CATGG
3885 CCATGG
NsiI 2050 ATGCAT ATGCA^T
2523 ATGCAT
NspV 798 TTCGAA TT^CGAA
2309 TTCGAA
PpuMI 2968 AGGTCCT [GA]G^G[AT]CC[CT]
3440 AGGACCT
RcaI 137 TCATGA T^CATGA
1505 TCATGA
SbfI 851 CCTGCAGG CCTGCA^GG
1059 CCTGCAGG
SexAI 780 ACCAGGT A^CC[AT]GGT
3571 ACCAGGT
SfuI 798 TTCGAA TT^CGAA
2309 TTCGAA
Sse8387I 851 CCTGCAGG CCTGCA^GG
1059 CCTGCAGG
SspI 3004 AATATT AAT^ATT
3365 AATATT
SwaI 1448 ATTTAAAT ATTT^AAAT
2470 ATTTAAAT
XmnI 324 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3876 GAATCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII