##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AviII BbeI BcgI BclI
BfrI BglI BglII BsaBI BsaHI
BsiEI BspDI BsrBI BsrDI BstAPI
BstBI ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I FbaI FseI
FspI HaeII Hin1I HincII HindII
HpaI KasI KspI MamI MfeI
MunI NaeI NarI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 4228 GGTACC G^GTACC
AccIII 1240 TCCGGA T^CCGGA
Asp718I 4228 GGTACC G^GTACC
BalI 3065 TGGCCA TGG^CCA
BamHI 1237 GGATCC G^GATCC
BseAI 1240 TCCGGA T^CCGGA
BsiWI 2695 CGTACG C^GTACG
BsmI 3258 GCATTC GAATGC(1/-1)
Bsp1407I 1694 TGTACA T^GTACA
BspEI 1240 TCCGGA T^CCGGA
BspHI 1996 TCATGA T^CATGA
BspLU11I 3561 ACATGT A^CATGT
BsrFI 966 ACCGGC [GA]^CCGG[CT]
BsrGI 1694 TGTACA T^GTACA
BssHII 194 GCGCGC G^CGCGC
BssSI 4364 CACGAG CACGAG(-5/-1)
Bst1107I 2713 GTATAC GTA^TAC
BstEII 4658 GGTGACC G^GT[ACGT]ACC
BstPI 4658 GGTGACC G^GT[ACGT]ACC
BstXI 685 CCATCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2713 GTATAC GTA^TAC
Cfr10I 966 ACCGGC [GA]^CCGG[CT]
EcoNI 2622 CCTCTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4658 GGTGACC G^GT[ACGT]ACC
EcoRI 2658 GAATTC G^AATTC
EcoRV 4290 GATATC GAT^ATC
EcoT22I 978 ATGCAT ATGCA^T
HindIII 3749 AAGCTT A^AGCTT
KpnI 4228 GGTACC GGTAC^C
MluI 2692 ACGCGT A^CGCGT
MluNI 3065 TGGCCA TGG^CCA
MroI 1240 TCCGGA T^CCGGA
MscI 3065 TGGCCA TGG^CCA
NdeI 291 CATATG CA^TATG
NheI 2903 GCTAGC G^CTAGC
NsiI 978 ATGCAT ATGCA^T
PstI 4379 CTGCAG CTGCA^G
RcaI 1996 TCATGA T^CATGA
SacI 1737 GAGCTC GAGCT^C
SpeI 645 ACTAGT A^CTAGT
SspBI 1694 TGTACA T^GTACA
StuI 2030 AGGCCT AGG^CCT
TaqI 1153 TCGA T^CGA
TthHB8I 1153 TCGA T^CGA
XbaI 3262 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 2633 AAATTT [GA]^AATT[CT]
2658 GAATTC
AhdI 450 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2009 GACAATGGGTC
AocI 3762 CCTCAGG CC^T[ACGT]AGG
4774 CCTAAGG
ApaI 404 GGGCCC GGGCC^C
4338 GGGCCC
ApoI 2633 AAATTT [GA]^AATT[CT]
2658 GAATTC
AspEI 450 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2009 GACAATGGGTC
AspHI 225 GAGCAC G[AT]GC[AT]^C
1737 GAGCTC
AspI 270 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3673 GACCGAGTC
AvrII 4322 CCTAGG C^CTAGG
4389 CCTAGG
BanI 1174 GGTGCC G^G[CT][GA]CC
4228 GGTACC
BbrPI 1774 CACGTG CAC^GTG
4169 CACGTG
BciVI 122 GGATAC GTATCC(6/5)
1461 GGATAC
BlnI 4322 CCTAGG C^CTAGG
4389 CCTAGG
BsaWI 898 ACCGGA [AT]^CCGG[AT]
1240 TCCGGA
BsiHKAI 225 GAGCAC G[AT]GC[AT]^C
1737 GAGCTC
Bsu36I 3762 CCTCAGG CC^T[ACGT]AGG
4774 CCTAAGG
DraI 2445 TTTAAA TTT^AAA
4595 TTTAAA
DraIII 165 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4264 CACTAAGTG
Eam1105I 450 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2009 GACAATGGGTC
Eco81I 3762 CCTCAGG CC^T[ACGT]AGG
4774 CCTAAGG
MslI 971 CACACGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2845 CACCCTGATG
PflFI 270 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3673 GACCGAGTC
PmaCI 1774 CACGTG CAC^GTG
4169 CACGTG
PmlI 1774 CACGTG CAC^GTG
4169 CACGTG
SapI 1734 GAAGAGC GCTCTTC(1/4)
3024 GAAGAGC
SmaI 3404 CCCGGG CCC^GGG
4728 CCCGGG
SphI 366 GCATGC GCATG^C
976 GCATGC
Tth111I 270 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3673 GACCGAGTC
XcmI 1198 CCAGAAGAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2576 CCAGATGTCCTGTGG
XmaCI 3404 CCCGGG C^CCGGG
4728 CCCGGG
XmaI 3404 CCCGGG C^CCGGG
4728 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AccII AciI AfaI AflIII
AluI AlwI AlwNI Asp700I AvaI
AvaII BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII
XmnI