################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII AclI AcyI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI AviII BbeI BcgI BclI BfrI BglI BglII BsaBI BsaHI BsiEI BspDI BsrBI BsrDI BstAPI BstBI ClaI CpoI DrdI EagI EclXI Eco47III Eco52I FbaI FseI FspI HaeII Hin1I HincII HindII HpaI KasI KspI MamI MfeI MunI NaeI NarI NcoI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 4228 GGTACC G^GTACC AccIII 1240 TCCGGA T^CCGGA Asp718I 4228 GGTACC G^GTACC BalI 3065 TGGCCA TGG^CCA BamHI 1237 GGATCC G^GATCC BseAI 1240 TCCGGA T^CCGGA BsiWI 2695 CGTACG C^GTACG BsmI 3258 GCATTC GAATGC(1/-1) Bsp1407I 1694 TGTACA T^GTACA BspEI 1240 TCCGGA T^CCGGA BspHI 1996 TCATGA T^CATGA BspLU11I 3561 ACATGT A^CATGT BsrFI 966 ACCGGC [GA]^CCGG[CT] BsrGI 1694 TGTACA T^GTACA BssHII 194 GCGCGC G^CGCGC BssSI 4364 CACGAG CACGAG(-5/-1) Bst1107I 2713 GTATAC GTA^TAC BstEII 4658 GGTGACC G^GT[ACGT]ACC BstPI 4658 GGTGACC G^GT[ACGT]ACC BstXI 685 CCATCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2713 GTATAC GTA^TAC Cfr10I 966 ACCGGC [GA]^CCGG[CT] EcoNI 2622 CCTCTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 4658 GGTGACC G^GT[ACGT]ACC EcoRI 2658 GAATTC G^AATTC EcoRV 4290 GATATC GAT^ATC EcoT22I 978 ATGCAT ATGCA^T HindIII 3749 AAGCTT A^AGCTT KpnI 4228 GGTACC GGTAC^C MluI 2692 ACGCGT A^CGCGT MluNI 3065 TGGCCA TGG^CCA MroI 1240 TCCGGA T^CCGGA MscI 3065 TGGCCA TGG^CCA NdeI 291 CATATG CA^TATG NheI 2903 GCTAGC G^CTAGC NsiI 978 ATGCAT ATGCA^T PstI 4379 CTGCAG CTGCA^G RcaI 1996 TCATGA T^CATGA SacI 1737 GAGCTC GAGCT^C SpeI 645 ACTAGT A^CTAGT SspBI 1694 TGTACA T^GTACA StuI 2030 AGGCCT AGG^CCT TaqI 1153 TCGA T^CGA TthHB8I 1153 TCGA T^CGA XbaI 3262 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcsI 2633 AAATTT [GA]^AATT[CT] 2658 GAATTC AhdI 450 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2009 GACAATGGGTC AocI 3762 CCTCAGG CC^T[ACGT]AGG 4774 CCTAAGG ApaI 404 GGGCCC GGGCC^C 4338 GGGCCC ApoI 2633 AAATTT [GA]^AATT[CT] 2658 GAATTC AspEI 450 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2009 GACAATGGGTC AspHI 225 GAGCAC G[AT]GC[AT]^C 1737 GAGCTC AspI 270 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3673 GACCGAGTC AvrII 4322 CCTAGG C^CTAGG 4389 CCTAGG BanI 1174 GGTGCC G^G[CT][GA]CC 4228 GGTACC BbrPI 1774 CACGTG CAC^GTG 4169 CACGTG BciVI 122 GGATAC GTATCC(6/5) 1461 GGATAC BlnI 4322 CCTAGG C^CTAGG 4389 CCTAGG BsaWI 898 ACCGGA [AT]^CCGG[AT] 1240 TCCGGA BsiHKAI 225 GAGCAC G[AT]GC[AT]^C 1737 GAGCTC Bsu36I 3762 CCTCAGG CC^T[ACGT]AGG 4774 CCTAAGG DraI 2445 TTTAAA TTT^AAA 4595 TTTAAA DraIII 165 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG 4264 CACTAAGTG Eam1105I 450 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2009 GACAATGGGTC Eco81I 3762 CCTCAGG CC^T[ACGT]AGG 4774 CCTAAGG MslI 971 CACACGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2845 CACCCTGATG PflFI 270 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3673 GACCGAGTC PmaCI 1774 CACGTG CAC^GTG 4169 CACGTG PmlI 1774 CACGTG CAC^GTG 4169 CACGTG SapI 1734 GAAGAGC GCTCTTC(1/4) 3024 GAAGAGC SmaI 3404 CCCGGG CCC^GGG 4728 CCCGGG SphI 366 GCATGC GCATG^C 976 GCATGC Tth111I 270 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3673 GACCGAGTC XcmI 1198 CCAGAAGAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2576 CCAGATGTCCTGTGG XmaCI 3404 CCCGGG C^CCGGG 4728 CCCGGG XmaI 3404 CCCGGG C^CCGGG 4728 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccI AccII AciI AfaI AflIII AluI AlwI AlwNI Asp700I AvaI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII XmnI