##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AflII AflIII AgeI AscI AseI
AsnI AspI AviII AvrII BbeI
BcgI BclI BfrI BlnI BsiWI
BsmBI BspDI BspLU11I Bst1107I BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoT22I FbaI FseI FspI HpaI
KasI KspI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PshBI PvuI RsrII SacII
SalI SfiI SfoI SgrAI SnaBI
SpeI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2100 GACGTC GACGT^C
AclI 4079 AACGTT AA^CGTT
AhdI 951 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3258 GTGCAC G^TGCAC
Aor51HI 36 AGCGCT AGC^GCT
ApaI 852 GGGCCC GGGCC^C
ApaLI 3258 GTGCAC G^TGCAC
AspEI 951 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1299 CACGTG CAC^GTG
BciVI 2609 GGATAC GTATCC(6/5)
BsaBI 322 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 189 CGGTCG CG[GA][CT]^CG
BspHI 2959 TCATGA T^CATGA
BssHII 179 GCGCGC G^CGCGC
BstEII 2709 GGTAACC G^GT[ACGT]ACC
BstPI 2709 GGTAACC G^GT[ACGT]ACC
DraI 5641 TTTAAA TTT^AAA
Eam1105I 951 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 36 AGCGCT AGC^GCT
EcoO65I 2709 GGTAACC G^GT[ACGT]ACC
EcoRV 5157 GATATC GAT^ATC
MamI 322 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 16 GCCGGC GCC^GGC
NcoI 6350 CCATGG C^CATGG
NgoMIV 16 GCCGGC G^CCGGC
PmaCI 1299 CACGTG CAC^GTG
PmlI 1299 CACGTG CAC^GTG
PshAI 3509 GACACGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4079 AACGTT AA^CGTT
RcaI 2959 TCATGA T^CATGA
SbfI 2906 CCTGCAGG CCTGCA^GG
ScaI 2992 AGTACT AGT^ACT
SexAI 3280 ACCAGGT A^CC[AT]GGT
SphI 1564 GCATGC GCATG^C
Sse8387I 2906 CCTGCAGG CCTGCA^GG
StuI 5193 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 519 GGTACC G^GTACC
933 GGTACC
AccIII 1786 TCCGGA T^CCGGA
2107 TCCGGA
Asp700I 2390 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2819 GAAGCTCTTC
Asp718I 519 GGTACC G^GTACC
933 GGTACC
BamHI 836 GGATCC G^GATCC
2245 GGATCC
BglI 16 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3669 GCCTTGATGGC
BglII 2944 AGATCT A^GATCT
3439 AGATCT
BseAI 1786 TCCGGA T^CCGGA
2107 TCCGGA
BspEI 1786 TCCGGA T^CCGGA
2107 TCCGGA
BsrFI 16 GCCGGC [GA]^CCGG[CT]
2855 GCCGGT
BstBI 781 TTCGAA TT^CGAA
3550 TTCGAA
Cfr10I 16 GCCGGC [GA]^CCGG[CT]
2855 GCCGGT
DraIII 221 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3848 CACCAAGTG
DsaI 3145 CCGTGG C^C[GA][CT]GG
6350 CCATGG
HincII 4163 GTTGAC GT[CT]^[GA]AC
6204 GTCAAC
HindII 4163 GTTGAC GT[CT]^[GA]AC
6204 GTCAAC
HindIII 4955 AAGCTT A^AGCTT
5442 AAGCTT
KpnI 519 GGTACC GGTAC^C
933 GGTACC
MroI 1786 TCCGGA T^CCGGA
2107 TCCGGA
NspI 1564 GCATGC [GA]CATG^[CT]
5256 GCATGT
NspV 781 TTCGAA TT^CGAA
3550 TTCGAA
SapI 2822 GCTCTTC GCTCTTC(1/4)
2840 GCTCTTC
SfuI 781 TTCGAA TT^CGAA
3550 TTCGAA
SspI 5309 AATATT AAT^ATT
6316 AATATT
TfiI 4533 GATTC G^A[AT]TC
4592 GATTC
XbaI 4595 TCTAGA T^CTAGA
5748 TCTAGA
XmnI 2390 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2819 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrGI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DrdI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspBI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI