################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AflII AflIII AgeI AscI AseI AsnI AspI AviII AvrII BbeI BcgI BclI BfrI BlnI BsiWI BsmBI BspDI BspLU11I Bst1107I BstZ17I ClaI CpoI EagI EclXI Eco52I EcoT22I FbaI FseI FspI HpaI KasI KspI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshBI PvuI RsrII SacII SalI SfiI SfoI SgrAI SnaBI SpeI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 2100 GACGTC GACGT^C AclI 4079 AACGTT AA^CGTT AhdI 951 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3258 GTGCAC G^TGCAC Aor51HI 36 AGCGCT AGC^GCT ApaI 852 GGGCCC GGGCC^C ApaLI 3258 GTGCAC G^TGCAC AspEI 951 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 1299 CACGTG CAC^GTG BciVI 2609 GGATAC GTATCC(6/5) BsaBI 322 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 189 CGGTCG CG[GA][CT]^CG BspHI 2959 TCATGA T^CATGA BssHII 179 GCGCGC G^CGCGC BstEII 2709 GGTAACC G^GT[ACGT]ACC BstPI 2709 GGTAACC G^GT[ACGT]ACC DraI 5641 TTTAAA TTT^AAA Eam1105I 951 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 36 AGCGCT AGC^GCT EcoO65I 2709 GGTAACC G^GT[ACGT]ACC EcoRV 5157 GATATC GAT^ATC MamI 322 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 16 GCCGGC GCC^GGC NcoI 6350 CCATGG C^CATGG NgoMIV 16 GCCGGC G^CCGGC PmaCI 1299 CACGTG CAC^GTG PmlI 1299 CACGTG CAC^GTG PshAI 3509 GACACGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 4079 AACGTT AA^CGTT RcaI 2959 TCATGA T^CATGA SbfI 2906 CCTGCAGG CCTGCA^GG ScaI 2992 AGTACT AGT^ACT SexAI 3280 ACCAGGT A^CC[AT]GGT SphI 1564 GCATGC GCATG^C Sse8387I 2906 CCTGCAGG CCTGCA^GG StuI 5193 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 519 GGTACC G^GTACC 933 GGTACC AccIII 1786 TCCGGA T^CCGGA 2107 TCCGGA Asp700I 2390 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2819 GAAGCTCTTC Asp718I 519 GGTACC G^GTACC 933 GGTACC BamHI 836 GGATCC G^GATCC 2245 GGATCC BglI 16 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3669 GCCTTGATGGC BglII 2944 AGATCT A^GATCT 3439 AGATCT BseAI 1786 TCCGGA T^CCGGA 2107 TCCGGA BspEI 1786 TCCGGA T^CCGGA 2107 TCCGGA BsrFI 16 GCCGGC [GA]^CCGG[CT] 2855 GCCGGT BstBI 781 TTCGAA TT^CGAA 3550 TTCGAA Cfr10I 16 GCCGGC [GA]^CCGG[CT] 2855 GCCGGT DraIII 221 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 3848 CACCAAGTG DsaI 3145 CCGTGG C^C[GA][CT]GG 6350 CCATGG HincII 4163 GTTGAC GT[CT]^[GA]AC 6204 GTCAAC HindII 4163 GTTGAC GT[CT]^[GA]AC 6204 GTCAAC HindIII 4955 AAGCTT A^AGCTT 5442 AAGCTT KpnI 519 GGTACC GGTAC^C 933 GGTACC MroI 1786 TCCGGA T^CCGGA 2107 TCCGGA NspI 1564 GCATGC [GA]CATG^[CT] 5256 GCATGT NspV 781 TTCGAA TT^CGAA 3550 TTCGAA SapI 2822 GCTCTTC GCTCTTC(1/4) 2840 GCTCTTC SfuI 781 TTCGAA TT^CGAA 3550 TTCGAA SspI 5309 AATATT AAT^ATT 6316 AATATT TfiI 4533 GATTC G^A[AT]TC 4592 GATTC XbaI 4595 TCTAGA T^CTAGA 5748 TCTAGA XmnI 2390 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2819 GAAGCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DrdI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI