##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI AviII AvrII BbeI BcgI
BciVI BclI BfrI BglII BlnI
BsaBI BseAI BseRI BsiEI BsiWI
BsmBI Bsp1407I BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoRI
EcoT22I FbaI FseI FspI HaeII
HincII HindII HpaI KasI Ksp632I
KspI MamI MfeI MluI MroI
MunI NarI NcoI NdeI NheI
NotI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tsp509I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 57 GGTACC G^GTACC
Alw44I 921 GTGCAC G^TGCAC
AocI 1139 CCTTAGG CC^T[ACGT]AGG
ApaI 564 GGGCCC GGGCC^C
ApaLI 921 GTGCAC G^TGCAC
Asp718I 57 GGTACC G^GTACC
AspI 1056 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1366 GGATCC G^GATCC
BbrPI 813 CACGTG CAC^GTG
BbvCI 35 GCTGAGG CCTCAGC(-5/-2)
BglI 810 GCCCACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1478 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1478 GCTCAGC GC^T[ACGT]AGC
BsaAI 813 CACGTG [CT]AC^GT[GA]
BsaWI 1363 ACCGGA [AT]^CCGG[AT]
BspDI 1050 ATCGAT AT^CGAT
BsrBI 20 CCGCTC CCGCTC(-3/-3)
BsrFI 26 GCCGGC [GA]^CCGG[CT]
BstEII 40 GGTTACC G^GT[ACGT]ACC
BstPI 40 GGTTACC G^GT[ACGT]ACC
Bsu36I 1139 CCTTAGG CC^T[ACGT]AGG
CelII 1478 GCTCAGC GC^T[ACGT]AGC
Cfr10I 26 GCCGGC [GA]^CCGG[CT]
ClaI 1050 ATCGAT AT^CGAT
Eco81I 1139 CCTTAGG CC^T[ACGT]AGG
EcoNI 350 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 40 GGTTACC G^GT[ACGT]ACC
EcoRV 402 GATATC GAT^ATC
EcoT14I 367 CCAAGG C^C[AT][AT]GG
HindIII 963 AAGCTT A^AGCTT
HphI 1118 GGTGA GGTGA(8/7)
KpnI 57 GGTACC GGTAC^C
MseI 316 TTAA T^TAA
NaeI 26 GCCGGC GCC^GGC
NgoMIV 26 GCCGGC G^CCGGC
NruI 788 TCGCGA TCG^CGA
PaeR7I 331 CTCGAG C^TCGAG
PflFI 1056 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 551 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 813 CACGTG CAC^GTG
PmlI 813 CACGTG CAC^GTG
PstI 652 CTGCAG CTGCA^G
SacI 390 GAGCTC GAGCT^C
ScaI 1297 AGTACT AGT^ACT
SfiI 809 GGCCCACGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1443 CCCGGG CCC^GGG
StyI 367 CCAAGG C^C[AT][AT]GG
Tru9I 316 TTAA T^TAA
Tth111I 1056 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 551 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 490 CCATGCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 331 CTCGAG C^TCGAG
XmaCI 1443 CCCGGG C^CCGGG
XmaI 1443 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 592 CGCG CG^CG
789 CGCG
AcyI 483 GACGCC G[GA]^CG[CT]C
795 GACGCC
BalI 817 TGGCCA TGG^CCA
874 TGGCCA
BfaI 546 CTAG C^TAG
1397 CTAG
BpmI 81 CTCCAG CTGGAG(16/14)
658 CTGGAG
BsaHI 483 GACGCC G[GA]^CG[CT]C
795 GACGCC
BsaI 96 GAGACC GGTCTC(1/5)
747 GGTCTC
BsmFI 833 GGGAC GGGAC(10/14)
1449 GTCCC
BsmI 1145 GAATGC GAATGC(1/-1)
528 GCATTC
BsrDI 821 CATTGC GCAATG(2/0)
274 GCAATG
BstUI 592 CGCG CG^CG
789 CGCG
BstYI 778 AGATCC [GA]^GATC[CT]
1366 GGATCC
Hin1I 483 GACGCC G[GA]^CG[CT]C
795 GACGCC
MaeI 546 CTAG C^TAG
1397 CTAG
MflI 778 AGATCC [GA]^GATC[CT]
1366 GGATCC
MluNI 817 TGGCCA TGG^CCA
874 TGGCCA
MscI 817 TGGCCA TGG^CCA
874 TGGCCA
MvnI 592 CGCG CG^CG
789 CGCG
PleI 429 GACTC GAGTC(4/5)
1121 GACTC
SmlI 331 CTCGAG C^T[CT][GA]AG
704 CTTGAG
SphI 152 GCATGC GCATG^C
518 GCATGC
TfiI 263 GAATC G^A[AT]TC
1253 GATTC
XhoII 778 AGATCC [GA]^GATC[CT]
1366 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BmyI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PpuMI PshAI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
TspRI TthHB8I