##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccII AgeI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BbrPI BcgI
BglI BlnI BsaAI BsaBI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoO65I EcoRV
FseI FspI HgaI KpnI KspI
MamI MluI MvnI NaeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 123 TCCGGA T^CCGGA
AclI 4798 AACGTT AA^CGTT
AcyI 1813 GGCGCC G[GA]^CG[CT]C
AflIII 5629 ACATGT A^C[GA][CT]GT
AhdI 908 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1850 AGCGCT AGC^GCT
Asp700I 3649 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 908 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1857 CTCGAG C^[CT]CG[GA]G
BamHI 3366 GGATCC G^GATCC
BbeI 1813 GGCGCC GGCGC^C
BciVI 5962 GTATCC GTATCC(6/5)
BclI 799 TGATCA T^GATCA
BlpI 337 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 337 GCTTAGC GC^T[ACGT]AGC
BsaHI 1813 GGCGCC G[GA]^CG[CT]C
BsaWI 123 TCCGGA [AT]^CCGG[AT]
BseAI 123 TCCGGA T^CCGGA
BsoBI 1857 CTCGAG C^[CT]CG[GA]G
BspEI 123 TCCGGA T^CCGGA
BspLU11I 5629 ACATGT A^CATGT
BsrBI 2077 GAGCGG CCGCTC(-3/-3)
BsrFI 5360 GCCGGT [GA]^CCGG[CT]
BssSI 29 CACGAG CACGAG(-5/-1)
CelII 337 GCTTAGC GC^T[ACGT]AGC
Cfr10I 5360 GCCGGT [GA]^CCGG[CT]
DraIII 528 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4034 GACTGAGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 908 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1850 AGCGCT AGC^GCT
EcoRI 1103 GAATTC G^AATTC
FbaI 799 TGATCA T^GATCA
Hin1I 1813 GGCGCC G[GA]^CG[CT]C
HincII 2204 GTTAAC GT[CT]^[GA]AC
HindII 2204 GTTAAC GT[CT]^[GA]AC
HpaI 2204 GTTAAC GTT^AAC
KasI 1813 GGCGCC G^GCGCC
MfeI 4385 CAATTG C^AATTG
MroI 123 TCCGGA T^CCGGA
MunI 4385 CAATTG C^AATTG
NarI 1813 GGCGCC GG^CGCC
NdeI 4850 CATATG CA^TATG
PaeR7I 1857 CTCGAG C^TCGAG
PshAI 827 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4798 AACGTT AA^CGTT
SfoI 1813 GGCGCC GGC^GCC
SphI 3579 GCATGC GCATG^C
XbaI 1172 TCTAGA T^CTAGA
XhoI 1857 CTCGAG C^TCGAG
XmnI 3649 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 416 CTTAAG C^TTAAG
3676 CTTAAG
ApaI 1396 GGGCCC GGGCC^C
4927 GGGCCC
BbvCI 5755 CCTCAGC CCTCAGC(-5/-2)
2104 GCTGAGG
BcnI 1901 CCCGG CC^[GC]GG
2970 CCCGG
BfrI 416 CTTAAG C^TTAAG
3676 CTTAAG
BstAPI 2040 GCAGGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2974 GCACAGCCTGC
CfoI 1814 GCGC GCG^C
1851 GCGC
EcoNI 1856 CCTCGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2817 CCTTCCAAAGG
EcoT22I 2336 ATGCAT ATGCA^T
3577 ATGCAT
FauI 2010 CCCGC CCCGC(4/6)
112 GCGGG
HaeII 1813 GGCGCC [GA]GCGC^[CT]
1850 AGCGCT
HhaI 1814 GCGC GCG^C
1851 GCGC
HinP1I 1814 GCGC G^CGC
1851 GCGC
HindIII 2719 AAGCTT A^AGCTT
5323 AAGCTT
MaeII 3952 ACGT A^CGT
4799 ACGT
NciI 1901 CCCGG CC^[GC]GG
2970 CCCGG
NsiI 2336 ATGCAT ATGCA^T
3577 ATGCAT
SapI 5144 GAAGAGC GCTCTTC(1/4)
4463 GCTCTTC
SexAI 3602 ACCTGGT A^CC[AT]GGT
5113 ACCTGGT
SspI 5003 AATATT AAT^ATT
6003 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII