################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AccII AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BbrPI BcgI BglI BlnI BsaAI BsaBI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoO65I EcoRV FseI FspI HgaI KpnI KspI MamI MluI MvnI NaeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 123 TCCGGA T^CCGGA AclI 4798 AACGTT AA^CGTT AcyI 1813 GGCGCC G[GA]^CG[CT]C AflIII 5629 ACATGT A^C[GA][CT]GT AhdI 908 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1850 AGCGCT AGC^GCT Asp700I 3649 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 908 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 1857 CTCGAG C^[CT]CG[GA]G BamHI 3366 GGATCC G^GATCC BbeI 1813 GGCGCC GGCGC^C BciVI 5962 GTATCC GTATCC(6/5) BclI 799 TGATCA T^GATCA BlpI 337 GCTTAGC GC^T[ACGT]AGC Bpu1102I 337 GCTTAGC GC^T[ACGT]AGC BsaHI 1813 GGCGCC G[GA]^CG[CT]C BsaWI 123 TCCGGA [AT]^CCGG[AT] BseAI 123 TCCGGA T^CCGGA BsoBI 1857 CTCGAG C^[CT]CG[GA]G BspEI 123 TCCGGA T^CCGGA BspLU11I 5629 ACATGT A^CATGT BsrBI 2077 GAGCGG CCGCTC(-3/-3) BsrFI 5360 GCCGGT [GA]^CCGG[CT] BssSI 29 CACGAG CACGAG(-5/-1) CelII 337 GCTTAGC GC^T[ACGT]AGC Cfr10I 5360 GCCGGT [GA]^CCGG[CT] DraIII 528 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4034 GACTGAGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 908 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1850 AGCGCT AGC^GCT EcoRI 1103 GAATTC G^AATTC FbaI 799 TGATCA T^GATCA Hin1I 1813 GGCGCC G[GA]^CG[CT]C HincII 2204 GTTAAC GT[CT]^[GA]AC HindII 2204 GTTAAC GT[CT]^[GA]AC HpaI 2204 GTTAAC GTT^AAC KasI 1813 GGCGCC G^GCGCC MfeI 4385 CAATTG C^AATTG MroI 123 TCCGGA T^CCGGA MunI 4385 CAATTG C^AATTG NarI 1813 GGCGCC GG^CGCC NdeI 4850 CATATG CA^TATG PaeR7I 1857 CTCGAG C^TCGAG PshAI 827 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 4798 AACGTT AA^CGTT SfoI 1813 GGCGCC GGC^GCC SphI 3579 GCATGC GCATG^C XbaI 1172 TCTAGA T^CTAGA XhoI 1857 CTCGAG C^TCGAG XmnI 3649 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflII 416 CTTAAG C^TTAAG 3676 CTTAAG ApaI 1396 GGGCCC GGGCC^C 4927 GGGCCC BbvCI 5755 CCTCAGC CCTCAGC(-5/-2) 2104 GCTGAGG BcnI 1901 CCCGG CC^[GC]GG 2970 CCCGG BfrI 416 CTTAAG C^TTAAG 3676 CTTAAG BstAPI 2040 GCAGGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2974 GCACAGCCTGC CfoI 1814 GCGC GCG^C 1851 GCGC EcoNI 1856 CCTCGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2817 CCTTCCAAAGG EcoT22I 2336 ATGCAT ATGCA^T 3577 ATGCAT FauI 2010 CCCGC CCCGC(4/6) 112 GCGGG HaeII 1813 GGCGCC [GA]GCGC^[CT] 1850 AGCGCT HhaI 1814 GCGC GCG^C 1851 GCGC HinP1I 1814 GCGC G^CGC 1851 GCGC HindIII 2719 AAGCTT A^AGCTT 5323 AAGCTT MaeII 3952 ACGT A^CGT 4799 ACGT NciI 1901 CCCGG CC^[GC]GG 2970 CCCGG NsiI 2336 ATGCAT ATGCA^T 3577 ATGCAT SapI 5144 GAAGAGC GCTCTTC(1/4) 4463 GCTCTTC SexAI 3602 ACCTGGT A^CC[AT]GGT 5113 ACCTGGT SspI 5003 AATATT AAT^ATT 6003 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII