################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AviII BbeI BbrPI BfrI BglII BsaAI BsaHI BspDI BspLU11I BssHII BstBI BstEII BstPI Bsu36I ClaI CpoI Eco47III Eco81I EcoO65I FseI FspI HgaI Hin1I HpaI KasI MfeI MluI MunI NarI NotI NruI NspV PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SbfI SfiI SfoI SfuI SgrAI SnaBI Sse8387I StuI SwaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 2375 TCCGGA T^CCGGA AclI 2665 AACGTT AA^CGTT BamHI 807 GGATCC G^GATCC BcgI 1104 GCAGGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1458 TGATCA T^GATCA BglI 5520 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 501 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2375 TCCGGA [AT]^CCGG[AT] BseAI 2375 TCCGGA T^CCGGA BsiEI 93 CGGCCG CG[GA][CT]^CG BsiWI 284 CGTACG C^GTACG BsmBI 769 GAGACG CGTCTC(1/5) Bsp1407I 503 TGTACA T^GTACA BspEI 2375 TCCGGA T^CCGGA BsrBI 21 GAGCGG CCGCTC(-3/-3) BsrFI 46 GCCGGC [GA]^CCGG[CT] BsrGI 503 TGTACA T^GTACA BssSI 1549 CACGAG CACGAG(-5/-1) Cfr10I 46 GCCGGC [GA]^CCGG[CT] DraI 1254 TTTAAA TTT^AAA DraIII 5149 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 93 CGGCCG C^GGCCG EclXI 93 CGGCCG C^GGCCG Eco52I 93 CGGCCG C^GGCCG EcoRI 3436 GAATTC G^AATTC EcoRV 1207 GATATC GAT^ATC EcoT22I 5467 ATGCAT ATGCA^T FbaI 1458 TGATCA T^GATCA HindIII 3055 AAGCTT A^AGCTT KspI 96 CCGCGG CCGC^GG MamI 501 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2375 TCCGGA T^CCGGA NaeI 46 GCCGGC GCC^GGC NdeI 5083 CATATG CA^TATG NgoMIV 46 GCCGGC G^CCGGC NheI 3735 GCTAGC G^CTAGC NsiI 5467 ATGCAT ATGCA^T PacI 5473 TTAATTAA TTAAT^TAA Psp1406I 2665 AACGTT AA^CGTT SacII 96 CCGCGG CCGC^GG SalI 3903 GTCGAC G^TCGAC ScaI 1349 AGTACT AGT^ACT SexAI 2265 ACCTGGT A^CC[AT]GGT SpeI 4341 ACTAGT A^CTAGT SspBI 503 TGTACA T^GTACA SspI 4876 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 100 GGTACC G^GTACC 3786 GGTACC AhdI 31 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3064 GACACCAAGTC ApaI 88 GGGCCC GGGCC^C 3688 GGGCCC Asp718I 100 GGTACC G^GTACC 3786 GGTACC AspEI 31 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3064 GACACCAAGTC AvrII 3884 CCTAGG C^CTAGG 4204 CCTAGG BbsI 5369 GAAGAC GAAGAC(2/6) 3641 GTCTTC BciVI 863 GTATCC GTATCC(6/5) 1919 GTATCC BlnI 3884 CCTAGG C^CTAGG 4204 CCTAGG BlpI 546 GCTTAGC GC^T[ACGT]AGC 3854 GCTAAGC Bpu1102I 546 GCTTAGC GC^T[ACGT]AGC 3854 GCTAAGC BpuAI 5369 GAAGAC GAAGAC(2/6) 3641 GTCTTC BspHI 734 TCATGA T^CATGA 5004 TCATGA Bst1107I 1955 GTATAC GTA^TAC 2135 GTATAC BstAPI 393 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 441 GCAATATCTGC BstZ17I 1955 GTATAC GTA^TAC 2135 GTATAC CelII 546 GCTTAGC GC^T[ACGT]AGC 3854 GCTAAGC DrdI 3397 GACTACTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5101 GACTGGGTGGTC Eam1105I 31 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3064 GACACCAAGTC HaeII 49 GGCGCT [GA]GCGC^[CT] 3500 AGCGCC HincII 3700 GTCAAC GT[CT]^[GA]AC 3903 GTCGAC HindII 3700 GTCAAC GT[CT]^[GA]AC 3903 GTCGAC KpnI 100 GGTACC GGTAC^C 3786 GGTACC NcoI 803 CCATGG C^CATGG 3764 CCATGG NspI 2642 GCATGC [GA]CATG^[CT] 2926 GCATGC PflMI 57 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5244 CCAGAGCATGG RcaI 734 TCATGA T^CATGA 5004 TCATGA SacI 483 GAGCTC GAGCT^C 2971 GAGCTC SmaI 104 CCCGGG CCC^GGG 4423 CCCGGG SphI 2642 GCATGC GCATG^C 2926 GCATGC Van91I 57 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5244 CCAGAGCATGG XbaI 900 TCTAGA T^CTAGA 3645 TCTAGA XmaCI 104 CCCGGG C^CCGGG 4423 CCCGGG XmaI 104 CCCGGG C^CCGGG 4423 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AspI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII XmnI