##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI AviII BbeI BbrPI
BfrI BglII BsaAI BsaHI BspDI
BspLU11I BssHII BstBI BstEII BstPI
Bsu36I ClaI CpoI Eco47III Eco81I
EcoO65I FseI FspI HgaI Hin1I
HpaI KasI MfeI MluI MunI
NarI NotI NruI NspV PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SbfI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 2375 TCCGGA T^CCGGA
AclI 2665 AACGTT AA^CGTT
BamHI 807 GGATCC G^GATCC
BcgI 1104 GCAGGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1458 TGATCA T^GATCA
BglI 5520 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 501 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2375 TCCGGA [AT]^CCGG[AT]
BseAI 2375 TCCGGA T^CCGGA
BsiEI 93 CGGCCG CG[GA][CT]^CG
BsiWI 284 CGTACG C^GTACG
BsmBI 769 GAGACG CGTCTC(1/5)
Bsp1407I 503 TGTACA T^GTACA
BspEI 2375 TCCGGA T^CCGGA
BsrBI 21 GAGCGG CCGCTC(-3/-3)
BsrFI 46 GCCGGC [GA]^CCGG[CT]
BsrGI 503 TGTACA T^GTACA
BssSI 1549 CACGAG CACGAG(-5/-1)
Cfr10I 46 GCCGGC [GA]^CCGG[CT]
DraI 1254 TTTAAA TTT^AAA
DraIII 5149 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 93 CGGCCG C^GGCCG
EclXI 93 CGGCCG C^GGCCG
Eco52I 93 CGGCCG C^GGCCG
EcoRI 3436 GAATTC G^AATTC
EcoRV 1207 GATATC GAT^ATC
EcoT22I 5467 ATGCAT ATGCA^T
FbaI 1458 TGATCA T^GATCA
HindIII 3055 AAGCTT A^AGCTT
KspI 96 CCGCGG CCGC^GG
MamI 501 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2375 TCCGGA T^CCGGA
NaeI 46 GCCGGC GCC^GGC
NdeI 5083 CATATG CA^TATG
NgoMIV 46 GCCGGC G^CCGGC
NheI 3735 GCTAGC G^CTAGC
NsiI 5467 ATGCAT ATGCA^T
PacI 5473 TTAATTAA TTAAT^TAA
Psp1406I 2665 AACGTT AA^CGTT
SacII 96 CCGCGG CCGC^GG
SalI 3903 GTCGAC G^TCGAC
ScaI 1349 AGTACT AGT^ACT
SexAI 2265 ACCTGGT A^CC[AT]GGT
SpeI 4341 ACTAGT A^CTAGT
SspBI 503 TGTACA T^GTACA
SspI 4876 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 100 GGTACC G^GTACC
3786 GGTACC
AhdI 31 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3064 GACACCAAGTC
ApaI 88 GGGCCC GGGCC^C
3688 GGGCCC
Asp718I 100 GGTACC G^GTACC
3786 GGTACC
AspEI 31 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3064 GACACCAAGTC
AvrII 3884 CCTAGG C^CTAGG
4204 CCTAGG
BbsI 5369 GAAGAC GAAGAC(2/6)
3641 GTCTTC
BciVI 863 GTATCC GTATCC(6/5)
1919 GTATCC
BlnI 3884 CCTAGG C^CTAGG
4204 CCTAGG
BlpI 546 GCTTAGC GC^T[ACGT]AGC
3854 GCTAAGC
Bpu1102I 546 GCTTAGC GC^T[ACGT]AGC
3854 GCTAAGC
BpuAI 5369 GAAGAC GAAGAC(2/6)
3641 GTCTTC
BspHI 734 TCATGA T^CATGA
5004 TCATGA
Bst1107I 1955 GTATAC GTA^TAC
2135 GTATAC
BstAPI 393 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
441 GCAATATCTGC
BstZ17I 1955 GTATAC GTA^TAC
2135 GTATAC
CelII 546 GCTTAGC GC^T[ACGT]AGC
3854 GCTAAGC
DrdI 3397 GACTACTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5101 GACTGGGTGGTC
Eam1105I 31 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3064 GACACCAAGTC
HaeII 49 GGCGCT [GA]GCGC^[CT]
3500 AGCGCC
HincII 3700 GTCAAC GT[CT]^[GA]AC
3903 GTCGAC
HindII 3700 GTCAAC GT[CT]^[GA]AC
3903 GTCGAC
KpnI 100 GGTACC GGTAC^C
3786 GGTACC
NcoI 803 CCATGG C^CATGG
3764 CCATGG
NspI 2642 GCATGC [GA]CATG^[CT]
2926 GCATGC
PflMI 57 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5244 CCAGAGCATGG
RcaI 734 TCATGA T^CATGA
5004 TCATGA
SacI 483 GAGCTC GAGCT^C
2971 GAGCTC
SmaI 104 CCCGGG CCC^GGG
4423 CCCGGG
SphI 2642 GCATGC GCATG^C
2926 GCATGC
Van91I 57 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5244 CCAGAGCATGG
XbaI 900 TCTAGA T^CTAGA
3645 TCTAGA
XmaCI 104 CCCGGG C^CCGGG
4423 CCCGGG
XmaI 104 CCCGGG C^CCGGG
4423 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AspI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflFI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII XmnI